The preparation, regeneration and mutagenesis of the taxol-producing fungus UV40-19 protoplasts were discussed in the experiment. Totally 42 strains displayed hygromycin resistance. Six strains were found to be positi...The preparation, regeneration and mutagenesis of the taxol-producing fungus UV40-19 protoplasts were discussed in the experiment. Totally 42 strains displayed hygromycin resistance. Six strains were found to be positive mutants when screened on plate containing 90μg/mL hygromycin. One hereditarily stable strain UN05-6 was obtained, which raised the taxol yield from (376.38 ± 8.41)μg/L to (493.12 ±11.36) μg/L. The optimal conditions for the preparation, regeneration and mutagenesis of the taxol producing fungus UV40-19 were as follows: 1 )enzymolysis in a solution containing 3% lywallzyme, 4% snailase, 1% lysozyme and 3% cellulose at 30~C water bath, pH5.5 - 6.0 for 5h; 2) The prepared protoplasts were regenerated by using bilayer plate culturing method; 3)To mutagenize the fungus UV40-19, the protoplast suspension was treated with 0.8mg/mL NTG for 15min, followed by UV irradiation (30W, 30cm distance)for 40s under magnetic stirring. The purified products of the fungus UN05-6 fermented extracts have significant inhibitive effects on SMMC-7721 cell.展开更多
Nowadays, people pass 90% of their time in closed spaces, therefore, an increasing care in the creation and upkeep of healthier environments as a personal and as professional level become a major issue. The present pr...Nowadays, people pass 90% of their time in closed spaces, therefore, an increasing care in the creation and upkeep of healthier environments as a personal and as professional level become a major issue. The present project has as goal the verification and optimization of the implicit need of an OCRAMclima~ AHU (air handling units) in IAQ (indoor air quality) and its effectiveness in treatment and/or air purification, having as basis the legal national and European requirements for IAQ. This work was based on the study of the state of the art of the techniques in air purification and in the evaluation of their performance, culminating in the production of the prototype OCRAMclima~ NPS (nano purifying system). The purifying phenomena involved are UVGI (ultraviolet germicidal irradiation) and catalytic ionization of air. The performance test was accomplished in a closed loop circuit, which results, obtained by an independent IAQ analyst, were satisfactory, indicating the viability of application of this system to indoor air disinfection. The outcome revealed that the conjugating of both phenomena, the air sterilizing by UV and catalytic ionization, is efficient when used for air purification, mainly for volatile organic compounds and bioaerosols.展开更多
In this work, the Au/ZnO hybrid microstructure was fabricated by assembling Au nanoparticles(NPs) onto the surface of ZnO microrods, and an obviously improved ultraviolet(UV) emission of ZnO is observed in the hybrid ...In this work, the Au/ZnO hybrid microstructure was fabricated by assembling Au nanoparticles(NPs) onto the surface of ZnO microrods, and an obviously improved ultraviolet(UV) emission of ZnO is observed in the hybrid microstructure. About 27-fold enhancement ratio of the UV emission to the green band emission of ZnO is achieved. The underlying enhanced mechanism of the UV emission intensities can be ascribed to the charge transfer and the efficient coupling between ZnO excitons and Au surface plasmon(SP).展开更多
The ZnO layer with thickness of 1.6 jim in ZnO/ZnGa2O4 composite structure was grown by the thermal oxidation of ZnS sub- strate with gallium. The optical property of the ZnO thick layer was investigated by time-resol...The ZnO layer with thickness of 1.6 jim in ZnO/ZnGa2O4 composite structure was grown by the thermal oxidation of ZnS sub- strate with gallium. The optical property of the ZnO thick layer was investigated by time-resolved photoluminescence. A single UV emission around 375 nm with short lifetime was observed at room temperature while the visible emission was absolutely quenched. The UV emission band was composed of the neutral donor bound exciton (D^0X) and donor-acceptor pair (DAP) emission peaks with large full-width at half-maximums (FWHMs) at 3.367 and 3.318 eV, respectively, at 10 K. However, the intensity of the D^0X emission was stronger than that of the DAP emission at measuring temperatures of 10-300 K.展开更多
基金Supported by the National Science Foundation of China (30570025)the Fifteen Important Items of Heilongjiang (GA02C101)+3 种基金Key Scientific Program of Harbin (011421126)Research Program for Scholars Overseas by Heilongjiang Education Bureau ( 1152HZ06)Harbin Youth Science Foundation (2005AFOXJ063)Outstanding Young Scientist Foundation of Heilongjiang University
文摘The preparation, regeneration and mutagenesis of the taxol-producing fungus UV40-19 protoplasts were discussed in the experiment. Totally 42 strains displayed hygromycin resistance. Six strains were found to be positive mutants when screened on plate containing 90μg/mL hygromycin. One hereditarily stable strain UN05-6 was obtained, which raised the taxol yield from (376.38 ± 8.41)μg/L to (493.12 ±11.36) μg/L. The optimal conditions for the preparation, regeneration and mutagenesis of the taxol producing fungus UV40-19 were as follows: 1 )enzymolysis in a solution containing 3% lywallzyme, 4% snailase, 1% lysozyme and 3% cellulose at 30~C water bath, pH5.5 - 6.0 for 5h; 2) The prepared protoplasts were regenerated by using bilayer plate culturing method; 3)To mutagenize the fungus UV40-19, the protoplast suspension was treated with 0.8mg/mL NTG for 15min, followed by UV irradiation (30W, 30cm distance)for 40s under magnetic stirring. The purified products of the fungus UN05-6 fermented extracts have significant inhibitive effects on SMMC-7721 cell.
文摘Nowadays, people pass 90% of their time in closed spaces, therefore, an increasing care in the creation and upkeep of healthier environments as a personal and as professional level become a major issue. The present project has as goal the verification and optimization of the implicit need of an OCRAMclima~ AHU (air handling units) in IAQ (indoor air quality) and its effectiveness in treatment and/or air purification, having as basis the legal national and European requirements for IAQ. This work was based on the study of the state of the art of the techniques in air purification and in the evaluation of their performance, culminating in the production of the prototype OCRAMclima~ NPS (nano purifying system). The purifying phenomena involved are UVGI (ultraviolet germicidal irradiation) and catalytic ionization of air. The performance test was accomplished in a closed loop circuit, which results, obtained by an independent IAQ analyst, were satisfactory, indicating the viability of application of this system to indoor air disinfection. The outcome revealed that the conjugating of both phenomena, the air sterilizing by UV and catalytic ionization, is efficient when used for air purification, mainly for volatile organic compounds and bioaerosols.
基金supported by the National Natural Science Foundation of China(No.U1404824)the Science&Technology Agency of Henan Province(No.142107000023)the Young Scientists Fund of Henan University of Science and Technology(No.09001635)
文摘In this work, the Au/ZnO hybrid microstructure was fabricated by assembling Au nanoparticles(NPs) onto the surface of ZnO microrods, and an obviously improved ultraviolet(UV) emission of ZnO is observed in the hybrid microstructure. About 27-fold enhancement ratio of the UV emission to the green band emission of ZnO is achieved. The underlying enhanced mechanism of the UV emission intensities can be ascribed to the charge transfer and the efficient coupling between ZnO excitons and Au surface plasmon(SP).
基金supported by the National Natural Science Foundation of China(Grant No.51202191)Natural Science Basic Research Plan in Shaanxi Province of China(Grant No.2012JQ6002)Scientific Research Program Funded by Shaanxi Provincial Education Department of China(Grant No.12JK0427)
文摘The ZnO layer with thickness of 1.6 jim in ZnO/ZnGa2O4 composite structure was grown by the thermal oxidation of ZnS sub- strate with gallium. The optical property of the ZnO thick layer was investigated by time-resolved photoluminescence. A single UV emission around 375 nm with short lifetime was observed at room temperature while the visible emission was absolutely quenched. The UV emission band was composed of the neutral donor bound exciton (D^0X) and donor-acceptor pair (DAP) emission peaks with large full-width at half-maximums (FWHMs) at 3.367 and 3.318 eV, respectively, at 10 K. However, the intensity of the D^0X emission was stronger than that of the DAP emission at measuring temperatures of 10-300 K.