Aim To separate high purity linolenic acid from the oil of Lithospermumerythrorhizon growing in the Northeast of China. Methods Urea inclusion and column chromatographywere used. Results Unsaturated fatty acid was sep...Aim To separate high purity linolenic acid from the oil of Lithospermumerythrorhizon growing in the Northeast of China. Methods Urea inclusion and column chromatographywere used. Results Unsaturated fatty acid was separated, with a purity of 99.30 wt% of linolenicacid. Conclusion The experiment shows excellent reproducibility and high feasibility for industrialproduction.展开更多
Examining the pollination biology of plant species is not only crucial for enhancing our understanding of their reproductive biology,but also essential for elucidating their adaptation and evolutionary history.Here,we...Examining the pollination biology of plant species is not only crucial for enhancing our understanding of their reproductive biology,but also essential for elucidating their adaptation and evolutionary history.Here,we investigated the breeding system and pollination biology of two closely related species in Actaea.The flower of A.purpurea is unique in the genus with purple and chartaceous(paper-like)sepals,fewer stamens with yellow anthers and purple filaments.Through three seasons of field observation and exclusion experiments,we determined that A.purpurea was primarily pollinated by a hornet species,Vespa bicolor,which also served as the most efficient pollinator.In contrast,A.japonica was primarily pollinated by large flies.Actaea purpurea exhibited a significantly higher cumulative nectar volume than A.japonica,which could be a crucial factor in attracting V.bicolor.A control experiment further demonstrated that removing the nectar leaf(petal)significantly decreased the visiting frequency of V.bicolor.Breeding system studies revealed that both species were self-compatible,yet they primarily underwent outcrossing in natural habitats.Our study presents a compelling case of possible pollinator shift in A.purpurea accompanied by morphological divergence.A more in-depth investigation of this system would offer crucial insights into the extent to which pollinators are involved in the plant speciation process and whether they contribute to reproductive isolation between closely related species.展开更多
文摘Aim To separate high purity linolenic acid from the oil of Lithospermumerythrorhizon growing in the Northeast of China. Methods Urea inclusion and column chromatographywere used. Results Unsaturated fatty acid was separated, with a purity of 99.30 wt% of linolenicacid. Conclusion The experiment shows excellent reproducibility and high feasibility for industrialproduction.
基金supported by the National Natural Science Foundation of China(31870194)the Innovation Capability Support Program of Shaanxi(Program No.2023KJXX-019)the Fundamental Research Funds for the Central Universities(GK202301008 to J.Q.Zhang).
文摘Examining the pollination biology of plant species is not only crucial for enhancing our understanding of their reproductive biology,but also essential for elucidating their adaptation and evolutionary history.Here,we investigated the breeding system and pollination biology of two closely related species in Actaea.The flower of A.purpurea is unique in the genus with purple and chartaceous(paper-like)sepals,fewer stamens with yellow anthers and purple filaments.Through three seasons of field observation and exclusion experiments,we determined that A.purpurea was primarily pollinated by a hornet species,Vespa bicolor,which also served as the most efficient pollinator.In contrast,A.japonica was primarily pollinated by large flies.Actaea purpurea exhibited a significantly higher cumulative nectar volume than A.japonica,which could be a crucial factor in attracting V.bicolor.A control experiment further demonstrated that removing the nectar leaf(petal)significantly decreased the visiting frequency of V.bicolor.Breeding system studies revealed that both species were self-compatible,yet they primarily underwent outcrossing in natural habitats.Our study presents a compelling case of possible pollinator shift in A.purpurea accompanied by morphological divergence.A more in-depth investigation of this system would offer crucial insights into the extent to which pollinators are involved in the plant speciation process and whether they contribute to reproductive isolation between closely related species.
