丹参饮水提液絮凝纯化产物HPLC指纹图谱分析

目的建立丹参饮水提液絮凝纯化产物的HPLC指纹图谱。方法采用HPLC法,色谱柱为Inertsil ODS-SP Extend C18（250 mm×4.6 mm,5.0μm）。乙腈为流动相A,0.5%冰醋酸溶液为流动相B,进行梯度洗脱,0-20 min,5%-15%A;20-60 min,15%-45%A。检测波长为281 nm,流速1.0 m L/min,柱温25℃。结果丹参饮水提液絮凝纯化物HPLC指纹图谱标定了9个共有峰,指认8号峰为丹酚酸B,18批样品的相似度均〉0.990。结论建立的丹参饮絮凝纯化物指纹图谱特征性及专属性强,可为其质量控制提供参考。

压裂返排液中不同铁基电极除硼性能的影响与分析

压裂返排液中硼的去除对非常规油气资源的可持续发展具有重要意义。电絮凝作为一种环保技术,近年来受到越来越多的关注。从电化学的角度来看,不同电极的电化学性能具有一定差异,这很大程度上影响着絮凝性能。然而,铁基电极作为经济有效的电极材料,不同铁基电极对除硼效果的影响至今还没有得到系统的研究。本研究采用不同的铁基电极作为阳极,考察了其在电絮凝过程中的除硼性能,并用电化学方法、化学方法及相关表征手段阐述了铁基电极产生不同除硼效果的原因。结果表明,相同条件下,不锈钢作为阳极时的除硼效率为17%左右,比碳钢作为阳极的除硼率大致高出10%。当使用不锈钢电极时,其所在介质的Fe^(3+)含量为43%,而碳钢电解时,Fe^(3+)含量却仅有30%。此外,不锈钢阳极电解产生的絮凝物具有更分散疏松的形态,粒径分布较为均一,有利于得到更好的絮凝能力。

Screening of a Novel Bioflocculant-producing Strain and Research on Its Flocculation

[Objective] This study aimed to screen a bacterial strain capable of producing bioflocculant. [Method] A bacterial strain T-11 capable of producing bioflocculant was isolated from activated sludge. Detailed tests on the morphological, physiological and biochemical characteristics were carried out and identification was performed to identify the strain. Finally, the bioflocculant was isolated and purified, and the flocculating activity and chemical characteristics were measured. [Result] It was identified as Serratia plumuthica based on its morphological, physiological and biochemical characteristics. This strain secreted flocculant best in a culture medium which included sucrose and NaNO3. The maximal cell growth was achieved within 10 h and the flocculating activity paralleled to it. It was found to be effective for flocculation of kaolin suspension, when added at a final concentration of 0.7 mg/L, over a range of pHs （2-7）, and temperature （approximately 30-80 ℃）. Chemical analysis indicated that the bioflocculant was an acidic polysaccharide consisting of glucose, glucuronic acid and galactose, talose and altrose. Infrared spectrum analysis also revealed typical characteristics of polysaccharides. [Conclusion] The biofloccu- lants produced by strain T-11 can greatly improve the ability of activated sludge to settle.

The flocculation efficiency of compound bioflocculant by flocculant-producing bacteria

The flucculation efficiency of compound bioflocculant produced by flocculant-producing bacteria was investigated in this study. Cheap cellulose was selected as the substrate for the production of a lower cost bioflocculant. The end product of cellulose decomposing bacteria was utilized as substrate for flocculant-producing bacteria. The optimum fermentation conditions were determined as follows： the initial fermentation and fermentation time was 5 d and 1 d respectively, the temperature was 30 ℃, the rotation speed was 120 r/min, the amount of CaCl2 solution （ 10% ） was 1.5 ml/L. The flocculation test indicated that the bioflocculant had high efficiency in the removal of the turbldity raw water from Songhua River.