In this study, non radioactive Digoxigenin labeled ribosomal DNA(rDNA) probes were used for Southern blotting analysis to study the molecular phylogeny of the giant panda and related species. Restriction maps in the ...In this study, non radioactive Digoxigenin labeled ribosomal DNA(rDNA) probes were used for Southern blotting analysis to study the molecular phylogeny of the giant panda and related species. Restriction maps in the regions of rDNA spacers were compared between giant panda( Ailuropoda melanoleuca ), lesser panda( Ailurus fulgens ), Asiatic black bear( Selenarctos thibetanus ), sun bear( Helarctos malayanus ), raccoon( Procyon lotor ) and lynx( Felis lynx ). Phylogenetic trees for these species were constructed using maximum likelihood and parsimony method. The results show that in respect to rDNA RFLPs, the giant panda is more closely related to bear than to lesser panda; while the lesser panda is slightly related to the raccoon.展开更多
Using the high sensitivity to initial values of chaotic systems, this paper describes an application of chaos in the field of measurement. A general method for signal coding based on symbolic sequences and the relatio...Using the high sensitivity to initial values of chaotic systems, this paper describes an application of chaos in the field of measurement. A general method for signal coding based on symbolic sequences and the relationship between the variable (to be measured) and its symbolic sequence are presented. Some performances of the chaos based measurement system are also discussed. Theoretical analysis and experimental results show that chaotic systems are potentially attractive in the field of measurement.展开更多
[Objective] The aim was to explore the phylogenetic relationships of Cara- gana （Fabaceae） by the use of nrlTS sequences. [Method] Internal transcribed spac- er （ITS） sequences of nuclear ribosomal DNA （nrDNA） f...[Objective] The aim was to explore the phylogenetic relationships of Cara- gana （Fabaceae） by the use of nrlTS sequences. [Method] Internal transcribed spac- er （ITS） sequences of nuclear ribosomal DNA （nrDNA） from 29 taxa of Caragana species and seven close relatives （all belong to Astralinae （Adens） Benth） were used for phylogenetic analysis. [Result] Length of the entire ITS region ranges from 611 to 614 bp in Caragana species. The aligned sequences nrlTS of Caragana are 655 bp, and 170 sites are variable, with 107 phylogenetically informative sites. The phylogenetically informative sites are 16.3% of the total aligned sequences. The ITS sequences data are useful to resolve some relationships at lower taxonomic levels within Caragana. The Caragana Fabr. is not a monophyletic group with very close connection with Calophaca tianschanica. The ITS data revealed that the species of Sect. tragacanthoides were dispersed in MP tree or ME tree. Although the morphol- ogy of C. ordosica is similar to C. tibetica, the ITS results revealed an unexpectedly close relativeship to C. roborovskyi. The ITS data also indicate C. davazamcii, C. korshinskii, C. intermedia, and C. microphylla are different species. [Conclusion] ITS sequences have an important reference value in exploring the relationships of Cara- gana.展开更多
The progress of grey system models is reviewed, and the general grey numbers, the grey sequence op- erators and several most commonly used grey system models are introduced, such as the absolute degree of grey inciden...The progress of grey system models is reviewed, and the general grey numbers, the grey sequence op- erators and several most commonly used grey system models are introduced, such as the absolute degree of grey incidence model, the grey cluster model based on endpoint triangular whitenization functions, the grey cluster model based on center-point triangular whitenization functions, the grey prediction model of the model GM （ 1,1）, and the weighted multi-attribute grey target decision model.展开更多
The internal transcribed spacers (ITS) (including ITS1, 5.8S rRNA gene and ITS2) of Caragana roborovskyi and eight species of subgenus Pogonophace ( Astragalus) were sequenced, and analyzed together with other ITS dat...The internal transcribed spacers (ITS) (including ITS1, 5.8S rRNA gene and ITS2) of Caragana roborovskyi and eight species of subgenus Pogonophace ( Astragalus) were sequenced, and analyzed together with other ITS data (from GenBank) of 48 species representing Astragalus and 12 related genera. The results of phylogenctic analyses suggest that the subgenus Pogonophace is not a monophyletic group. Sect. Sesbanella (Astragalus hoantchy and A. dshimensis) and other species of Pogonophace are nested within different major clades in the phylogenetic tree. The species of sections Bibracteola, Phyllolobium and Trichostylus compose a monophyletic group with a close relationship to subtribe Coluteinae instead of Astragalus. Astragalus tribulifolius and A. tanguticus might be a vicariant species pair as inferred by the ITS data.展开更多
[ Objective] Study on the phylogenetic analysis of the 16S rDNA and insecticidal characteristics of strain BH-1 isolated from diseased larva of Anoplophora glabripennis （Motseh.） [ Method ] The strain was identified...[ Objective] Study on the phylogenetic analysis of the 16S rDNA and insecticidal characteristics of strain BH-1 isolated from diseased larva of Anoplophora glabripennis （Motseh.） [ Method ] The strain was identified by routine method and inoculated onto healthy Anoplophora glabripennis （Motseh.） for observing insecticidal effect, further 16S DNA was amplified by the specific primers for sequencing and homology analysis. [ Result] The mortality of second instar ofAnoplophora glabripennis（ Motseh. ） reached 72.7% 8 d after 10^10cfu/ml BH-1 was inoculated. The homology of 16S DNA sequences between BH-1 and Serratia marcescens accessed in GenBank reached 99.5%. Combined with the results of routine identification, BH-1 was identified as S. marcescens. [Conclusion] BH-1 could be used for biological control ofAnoplophora glabripennis （Motsch.）.展开更多
文摘In this study, non radioactive Digoxigenin labeled ribosomal DNA(rDNA) probes were used for Southern blotting analysis to study the molecular phylogeny of the giant panda and related species. Restriction maps in the regions of rDNA spacers were compared between giant panda( Ailuropoda melanoleuca ), lesser panda( Ailurus fulgens ), Asiatic black bear( Selenarctos thibetanus ), sun bear( Helarctos malayanus ), raccoon( Procyon lotor ) and lynx( Felis lynx ). Phylogenetic trees for these species were constructed using maximum likelihood and parsimony method. The results show that in respect to rDNA RFLPs, the giant panda is more closely related to bear than to lesser panda; while the lesser panda is slightly related to the raccoon.
文摘Using the high sensitivity to initial values of chaotic systems, this paper describes an application of chaos in the field of measurement. A general method for signal coding based on symbolic sequences and the relationship between the variable (to be measured) and its symbolic sequence are presented. Some performances of the chaos based measurement system are also discussed. Theoretical analysis and experimental results show that chaotic systems are potentially attractive in the field of measurement.
基金Supported by National Key Basic Research Development Program of China(2002CB111505)~~
文摘[Objective] The aim was to explore the phylogenetic relationships of Cara- gana （Fabaceae） by the use of nrlTS sequences. [Method] Internal transcribed spac- er （ITS） sequences of nuclear ribosomal DNA （nrDNA） from 29 taxa of Caragana species and seven close relatives （all belong to Astralinae （Adens） Benth） were used for phylogenetic analysis. [Result] Length of the entire ITS region ranges from 611 to 614 bp in Caragana species. The aligned sequences nrlTS of Caragana are 655 bp, and 170 sites are variable, with 107 phylogenetically informative sites. The phylogenetically informative sites are 16.3% of the total aligned sequences. The ITS sequences data are useful to resolve some relationships at lower taxonomic levels within Caragana. The Caragana Fabr. is not a monophyletic group with very close connection with Calophaca tianschanica. The ITS data revealed that the species of Sect. tragacanthoides were dispersed in MP tree or ME tree. Although the morphol- ogy of C. ordosica is similar to C. tibetica, the ITS results revealed an unexpectedly close relativeship to C. roborovskyi. The ITS data also indicate C. davazamcii, C. korshinskii, C. intermedia, and C. microphylla are different species. [Conclusion] ITS sequences have an important reference value in exploring the relationships of Cara- gana.
基金Supported by the Joint Research Project of Both the National Natural Science Foundation of Chinaand the Royal Society(RS)of UK(71111130211)the National Natural Science Foundation of China(90924022,70971064,70901041,71171113)+7 种基金the Major Project of Social Science Foundation of China(10ZD&014)the Key Project of Social Science Foundation of China(08AJY024)the Key Project of Soft Science Foundation of China(2008GXS5D115)the Foundation of Doctoral Programs(200802870020,200902870032)the Foundation of Humanities and Social Sciences of Chinese National Ministry of Education(08JA630039)the Science Foundation ofthe Excellent and Creative Group of Science and Technology in Jiangsu Province(Y0553-091)the Foundation of Key Research Base of Philosophy and Social Science in Colleges and Universities of Jiangsu Province(2010JDXM015)the Foundation of Outstanding Teaching Group of China(10td128)~~
文摘The progress of grey system models is reviewed, and the general grey numbers, the grey sequence op- erators and several most commonly used grey system models are introduced, such as the absolute degree of grey incidence model, the grey cluster model based on endpoint triangular whitenization functions, the grey cluster model based on center-point triangular whitenization functions, the grey prediction model of the model GM （ 1,1）, and the weighted multi-attribute grey target decision model.
文摘The internal transcribed spacers (ITS) (including ITS1, 5.8S rRNA gene and ITS2) of Caragana roborovskyi and eight species of subgenus Pogonophace ( Astragalus) were sequenced, and analyzed together with other ITS data (from GenBank) of 48 species representing Astragalus and 12 related genera. The results of phylogenctic analyses suggest that the subgenus Pogonophace is not a monophyletic group. Sect. Sesbanella (Astragalus hoantchy and A. dshimensis) and other species of Pogonophace are nested within different major clades in the phylogenetic tree. The species of sections Bibracteola, Phyllolobium and Trichostylus compose a monophyletic group with a close relationship to subtribe Coluteinae instead of Astragalus. Astragalus tribulifolius and A. tanguticus might be a vicariant species pair as inferred by the ITS data.
基金Co-constructed Program of Beijing Education Committee for Scientific Research BaseResearch Fund for the Doctoral Program of Higher Education (20030022015)Program for Changjiang Scholars and Innovative Research Team in University (PCSIRT0607)~~
文摘[ Objective] Study on the phylogenetic analysis of the 16S rDNA and insecticidal characteristics of strain BH-1 isolated from diseased larva of Anoplophora glabripennis （Motseh.） [ Method ] The strain was identified by routine method and inoculated onto healthy Anoplophora glabripennis （Motseh.） for observing insecticidal effect, further 16S DNA was amplified by the specific primers for sequencing and homology analysis. [ Result] The mortality of second instar ofAnoplophora glabripennis（ Motseh. ） reached 72.7% 8 d after 10^10cfu/ml BH-1 was inoculated. The homology of 16S DNA sequences between BH-1 and Serratia marcescens accessed in GenBank reached 99.5%. Combined with the results of routine identification, BH-1 was identified as S. marcescens. [Conclusion] BH-1 could be used for biological control ofAnoplophora glabripennis （Motsch.）.
