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自身免疫因素在绵羊繁殖过程中的作用机制研究进展
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作者 白音巴图 刘在霞 +9 位作者 何江峰 王标 马凤英 常晨城 郭丽丽 石彩霞 满达 刘永斌 王宇 张文广 《中国畜牧杂志》 CAS CSCD 北大核心 2023年第10期61-66,73,共7页
自身免疫是影响绵羊妊娠过程的重要因素。母体通过有序的免疫调节机制与自体或异体配子及半异基因胎儿相互作用,并通过免疫细胞及免疫调节因子的作用来实现母胎免疫耐受及对抗外来病原体的免疫平衡。绵羊体内促炎因子和抗炎因子的免疫... 自身免疫是影响绵羊妊娠过程的重要因素。母体通过有序的免疫调节机制与自体或异体配子及半异基因胎儿相互作用,并通过免疫细胞及免疫调节因子的作用来实现母胎免疫耐受及对抗外来病原体的免疫平衡。绵羊体内促炎因子和抗炎因子的免疫平衡改变及复杂的动态调节机制保证了绵羊配子发生、受精、胚胎植入子宫、维持妊娠及分娩的有序进行,其中免疫调节因子异常变化是导致繁殖障碍的一个重要原因。因此,全面了解绵羊繁殖周期完整的免疫图景具有十分重要的研究意义。本文详细阐述了绵羊繁殖妊娠各阶段的自身免疫影响因素及其调控机制的研究进展,以期为绵羊高效繁育提供理论借鉴。 展开更多
关键词 绵羊 繁殖免疫 母胎耐受 免疫细胞 细胞因子
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Purification and Immunocompetence Analysis of GP5 Protein in Porcine Reproductive and Respiratory Syndrome 被引量:4
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作者 陈妍 田宏 +2 位作者 尹双辉 尚佑军 刘湘涛 《Agricultural Science & Technology》 CAS 2009年第3期97-100,共4页
[Objective] The purification and immunocompetence of GPS protein in porcine reproductive and respiratory syndrome (PRRS) were analyzed in this study, which provided basis for establishing the corresponding serological... [Objective] The purification and immunocompetence of GPS protein in porcine reproductive and respiratory syndrome (PRRS) were analyzed in this study, which provided basis for establishing the corresponding serological method. [Method] The recombinant expression plasmid pGEX-6P-5 was transformed into BL21 and expressed after being induced with IPTG. The solubility analysis of expression products was carried out, and then the recombinant protein was purified for SDS-PAGE identification and Western-blot analysis. Finally, the recombinant antigen was used in the immune experiment of guinea pigs. [Result] The target protein content accounted for 30% of the total cells protein content according to the chromatography scanning, and the purity of target protein after purification reached 80%. The purified protein was analyzed by Western-blot and immune experiment of guinea pigs, and the results showed that the expressed protein had good reactionogenicity and immunogenicity. [Conclusion] This study provides materials for further studies on the function between PRRSV ORF5 gene and its editing protein, which also lays a foundation for porcine reproductive and respiratory syndrome virus genetic engineering products. 展开更多
关键词 Porcine reproductive and respiratory syndrome GP5 EXPRESSION PURIFICATION IMMUNOCOMPETENCE
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