红蛇定痛液的药效学研究初报

本文报道了红蛇定痛液的药效实验研究，通过动物实验，经腹腔注射红蛇定痛液能显著提高小鼠的热板法痛阈及降低醋酸所致扭体反应的发生率；同时对小鼠二甲苯致耳廓炎症也有明显抗炎作用，且对蟾蜍离体坐骨神经和家兔的脊神经均呈局部阻滞麻醉作用，其镇痛作用与临床关系有待进一步研究．

红蛇定痛液的抗过敏止痒实验研究

目的 研究红蛇定痛液抗过敏止痒作用。方法 以 4 -氨基吡啶 (4- AP)诱发小鼠舔体反应为指标 ,观察红蛇定痛液的抗过敏止痒作用。结果 红蛇定痛液组小鼠舔体次数明显减少 ,与对照组比较 ,差异有显著性 (P<0 .0 1) ;与肤轻松组比较 ,差异不明显 (P>0 .0 5 )。

红蛇定痛液抗炎作用研究

研究红蛇定痛液的抗炎作用 ,为临床应用提供佐证。方法 :用二甲苯致鼠耳肿胀法及角叉菜胶致大鼠足肿胀法观察药物作用。结果 :红蛇定痛液对鼠耳肿胀及大鼠足肿胀均有明显的抑制作用。结论 :红蛇定痛液有较好的抗炎作用。

红皮云杉(Picea koraiensis Nakai)人工更新与造林的初步研究

本文对红皮云杉(Picea koraiensis Naksi)全光造林和冠下人工更新的生长状况进行了调查和统计分析,结果表明,在造林初期,全光造林红皮云杉的高生长和全株叶量高于冠下更新。单位叶量内营养元素(N、P、K)的含量冠下更新红皮云杉高于全光造林,但全析叶内营养元素的总含量则全光造林高于冠下更新红皮云杉。测定并分析春季林内与林外主要小气候因子对红皮云杉幼树生长的影响。

Determination of Melamine by Infrared Spectroscopy Based on Nonlinear Modeling

[Objective] The aim was to develop a nonlinear model of quantitative analysis of melamine content by infrared spectroscopy and provide theoretical basis for the nondestructive detection of melamine. [Method] According to dynamics,mathematical modeling and optimization theory,linear and nonlinear models were respectively set up by taking an absorption peak of 1 550 cm-1 as characteristic absorption peak. [Result] The correlation coefficient of nonlinear model was 0.922 7 and the recovery was 96%,which showed that the nonlinear model was more accurate than linearity model with correlation coefficient of 0.904 9 and recovery of 557%. [Conclusion] It is feasible to determine melamine content by using the nonlinear model quantitatively.

Analysis of Protein Content in Panicum miliaceum L. Using Near Infrared Transmittance Spectroscopy

[Objective] To explore a method for the rapid determination of protein con- tent in grains of Panicum miliaceum L. [Method] The near infrared transmittance spec- troscopy （NITS） was used to build the mathematical models for the quantitative analy- sis of protein content in the grains. Four combinations of treatment that first derivative and second derivative were respectively combined with partial least squares （PLS） and modified partial least squares （MPLS） were set to compare their effects on the original transmission spectrum. [Result] The predicting effects of the 4 combinations were similar. The optimal combination was first derivative with MPLS, in which the average determination coefficient of validation （RSQ） was 0.880 6, correlation coeffi- cient of cross validation （1-VR） was 0.857 0, standard error of calibration （SEC） was 0.342 4, standard error of cross validation （SECV） was 0.375 1, and the standard er- ror of prediction （SEP） was 0.454. [Conclusion] The constructed NITS model is a rapid way for the determination of protein content in grains of P. miliaceum.

Preliminary Identification of Red Pigment and Positive Correlation between the Contents of Red Pigment and Total Saponins of Panax notoginseng Fruits

[Objective] This study aimed to identify red pigment of Panax notoginseng fruits and explore the correlation between pigment content and total saponins of the fruits. [Method] The red pigment of Panax notoginseng fruits was preliminarily identi- fied with specific color reactions and UV-vis spectra, and the contents of the pigment and total saponins were determined via spectrophotometry. [Result] The red hues of the fruits were contributed by anthocyanins and/or the anthocyanidins. The contents of anthocyanins and total saponins of the fruits both decreased along with thinning of the red hues. The content difference of the anthocyanins in fruits with different red hues reached extremely significant level, but that of total saponins just reached significant level. [Conclusion] The red pigment of P. notoginseng fruits is anthocyanins which are of extremely significant positive correlation with total saponins in contents.

Isolation and Identification of Anthracnose Pathogen from Fruit of Red Fuji Apple

The fruits of Red Fuji apple with anthracnose symptoms were collected and submitted to tissue isolation and culture. One strain of anthracnose pathogen （numbered as Acgl） was obtained, and it was identified by both morphological and molecular biological methods. According to the morphological characteristics of the colony and conidia and the results of rDNA-ITS sequence analysis, the Acgl strain was identified as Colletotrichum gloeosporioides.

Effects of magnesium and cooling rate on titanium phase transformation for production of TiO_2

X-ray diffraction（XRD） and crystal structure analysis were used to study the effects of Mg content and cooling rate on the titanium phase transformation of three types of titanium slag. The results indicate that in the rapid cooling process, the titanium phase is anosovite, whose chemical formula is MgnTi（3-n）O5（0n1）. In the slow cooling process, when the Mg content is high, anosovite transforms into karrooite MgTi2O5 structure; when the Mg content is low, karrooite MgTi2O5 and rutile TiO2 both exist. The stability of karrooite MgTi2O5 is better than that of anosovite MgnTi（3-n）O5. Slow cooling contributes to the doping of Mg into the anosovite crystal and stabilises the anosovite crystal structure.

Synthesis of Chiral Polyaniline Induced by Modified Hemoglobin

The synthesis of chiral polyaniline （PANI） induced by modified hemoglobin （Hb） was pro- foundly explored for the first time. Results revealed that after being separated, inactivated or immobilized, Hb can still induce the formation of chiral PANI successfully, suggesting that Hb can be used as the chiral inducers regardless of harsh reaction conditions. By examining the properties of PANI induced by modified Hb, it was found that Hb（inactivated）-PANI possessed excellent chirality, stability, and crystalline structure. The globin separated from Hb was demonstrated to have the ability of inducing the production of chiral PANI whereas the hematin from Hb had no capacity to direct enantio specificity for the PANI chains. Results indicated that Hb（immobilized）-PANI exhibited poor yield, doping state, and crys- talline structure, indicating that the immobilization of Hb by entrapment was not beneficial to the polymerization reaction. Results also showed that the structure of Hb may have significant effects on the morphologies of chiral PANI.

Sequencing and Analysis of ITS Sequences of Bangia atropurpurea

[Object]To providing new data for phylogenesis of Bangia atropurpurea by the sequencing of internal transcribed spacer（ITS region）of rDNA.[Method]B.atropurpurea was collected from Niangziguan Spring in Shanxi,the DNA was extracted and the primers were designed for PCR amplification so as to obtain ITS gene sequence.[Result]The homology of ITS region between B.atropurpurea and Porphyra oligospermatangia was 75%,which was 79% between B.atropurpurea and P.yezoensis.[Conclusion]Compared with other genes,the ITS sequence had a greater evolution rate.The differences in classification and distribution resulted in the sequence diversity.

Determination of Phytoene Content in Tomato Ketchup by HPLC

[Objective] The aim was to establish an HPLC method for determination of phytoene content in tomato ketchup. [Method] The chromatographic conditions were as follows： column, Agilent AT C18 （250 mm×4.6 mm, 5 μm）; mobile phase, methanol-THF （75：25）; detection wavelength, 287 nm; flow rate, 1.0 ml/min; column temperature, 30 ℃; sample size, 50 μl. [Result] There was a good linear relation- ship in the phytoene content range of 0.186-1.116μg. The average recovery rate was 103.8% with RSD of 1.47%. The phytoene content in the tomato ketchup sample was determined as 10.7 rag/100 g simple, accurate, rapid and reliable, and phytoene content in tomato ketchup. [Conclusion] The established method is it can be used for the determination of

板蓝根片质量标准的研究

目的 :为板蓝根片的质量控制寻找可靠的方法。方法 :用薄层色谱扫描法对质量控制进行试验。结果

Aggregate Stability and Its Relationship with Some Chemical Properties of Red Soils in Subtropical China

The stability of aggregates in the surface soil is crucial to soil erosion and runoff generation. Thus, to understand the stability and the breakdown mechanisms of soil aggregates as well as the relationship between aggregate stability and selected soil chemical properties, such as different forms of Fe and Al oxides, organic matter, CEC and clay content, the aggregates of slightly and severely eroded red soils derived from Quaternary red clay in subtropical China were analyzed using the routine wet sieving…

Construction of universal quantitative models for the determination of cefoperazone sodium/sulbactam sodium for injection from different manufacturers using near-infrared reflectance spectroscopy

To develop near-infrared （NIR） reflectance spectroscopic methods for the quantitative analysis of cefoperazone sodium/ sulbactam sodium from different manufacturers for injection powder medicaments. Various powders of cefoperazone sodium/ sulbactam sodium were directly analyzed by non-destructive NIR reflectance spectroscopy using the spectrometer EQUINOX55. Two quantitative methods via integrating sphere （IS） and fiberoptic probe （FOP） models were explored from 6 batches of commercial samples and 42 batches of laboratory samples at a content ranging from 30% to 70% for cefoperazone and 60% to 20% for sulbactam. The root mean square errors of cross validation （RMSECV） and the root mean square errors of prediction （RMSEP） of IS were 1.79% and 2.85%, respectively, for cefoperazone sodium, and were 1.86% and 3.08%, respectively, for sulbactam sodium; and those of FOP were 2.93% and 2.92%, respectively, for cefoperazone sodium, and were 2.23% and 3.01%, respectively, for sulbactam sodium. Based on the ICH guidelines and Ref. 12, the quantitative models were then evaluated in terms of specificity, linearity, accuracy, precision, robustness and model transferability. The non-destructive quantitative NIR methods used in this study are applicable for rapid analysis of injectable powdered drugs from different manufacturers.

Computer Simulation of Compensation Method for Infrared Focal Plane Array

A major motivation for this work is to investigate a method of computer simulation for compensating fixed pattern noise of the infrared focal plane arrays. A mathematical model of the output signal of focal plane array was established; a compensating algorithm utilizing reference source was derived and simulating programs were designed. The images of compensating process verify the influence of nonuniformity of responsibility and offset on fixed pattern noise. The result show that simulating method of investigating compensation technology for focal plane arrays is feasible, the generated images and methods can be used to the study of image recognition.

Identification of Taxus cuspidata Sieb.et Zucc endophytic fungi─ new recorded-genus-species of China and the metabolite

A total of 94 isolates of endophytic fungi were isolated from the bark of 200-yr.-old Taxus cuspidata Sieb. et Zucc. in the primeval forest of the Changbai Mountain Natural Reserve, and 19 species of endophytic fungi were identified. Among the taxa 2 new species and 1 new varied species as well as the 6 known species of China had been described in a previous article published on Journal of Forestry Research, Vol. 14 (4): 290-294 (2003). This paper made a detail description on the morpho-logical characteristics of the 9 new recorded-genus-species and 1 new record species with illustrations, including Staphy-lotrichum coccosporum Meyer et Nicot, Botryodema lateritium Papendoorf & Upadhyay, Oedocephalum glomerulosum (Rul-liard) Sacc., Trichosporonoides oedocephalis Haskins & Spencer, Beniowskia sphaeroidea (Kal Chbrenner et Caeke) Mason, Thermomyces verrucosus Pugh, Blakeman & Morgan-jones, Gilmaniella humicola Brron , Arthrinium sphaerosperma (Per.:Fr.) Ellis, Ambrosiella brunnea (Verrall) Batra, and Cytosporina notha Diedz (1 new record species). And the habitats, hosts, distribu-tions and fruiting periods were recorded for the new recorded-genus-species. The determination result of thin layer chroma-tography (TLC) showed that the liquid fermentation metabolites of the strains could not react positively with taxoids developer - the vanillic aldehyde and dilute bismuth potassium iodide.