Bio-oil is a new liquid fuel produced by fast pyrolysis,which is a promising technology to convert bio-mass into liquid. Pyrolytic lignin extracted from bio-oil,a fine powder,contributes to the instability of bio-oil....Bio-oil is a new liquid fuel produced by fast pyrolysis,which is a promising technology to convert bio-mass into liquid. Pyrolytic lignin extracted from bio-oil,a fine powder,contributes to the instability of bio-oil. The paper presents the structural features of three kinds of pyrolytic lignin extracted from bio-oil with different methods(WIF,HMM,and LMM) . The pyrolytic lignin samples are characterized by Fourier transform infrared spectrometer(FTIR) and X-ray photoelectron spectroscopy(XPS) . FTIR data indicate that the three pyrolytic lignin samples have similar functional groups,while the absorption intensity is different,and show characteristic vibra-tions of typical lignocellulosic material groups O H(3340-3380 cm-1) ,C H(2912-2929 cm-1) and C O(1652-1725 cm-1) . Comparison in the region(3340-3380 cm-1) indicates that WIF has more O H stretch groups than HMM and LMM. The carbon spectra are fitted to four peaks:C1,C C or C H,BE 283.5 eV;C2,C OR or C OH,BE 284.5-285.8 eV;C3,C O or HO C OR,BE 286.10-287.10 eV;C4,O C O,BE 287.5-287.7 eV. The absence of C1,C C or C H indicates the dominant polymerization structure of aro-matic carbon in pyrolytic lignin samples. For HMM and WIF,C2a and C2b can not be separated,so there is no free hydroxyl group in the samples. The oxygen peaks are also fitted to four peaks:O1,OH,BE = 530.3 eV;O2,RC O,BE 531.45-531.72 eV;O3,O C O,BE = 532.73-533.74 eV;O4,H2O,BE 535 eV. The absence of O1 and O4 indicates that little hydroxyl groups and adsorbed water are present in the samples.展开更多
Marsdeniae tenacissimae extract （MTE） has been used as an adjuvant medicine for cancer therapy for a long time. Although massive studies demonstrated its considerable anti-cancer effect, there is no research on its ...Marsdeniae tenacissimae extract （MTE） has been used as an adjuvant medicine for cancer therapy for a long time. Although massive studies demonstrated its considerable anti-cancer effect, there is no research on its influence on erythrocytes, which are firstly interacted with MTE in the circulation. To investigate the influence of MTE on erythrocytes, we used a flow cytometer to detect the MTE-treated alternations of morphology, calcium concentration, and reactive oxygen species （ROS） level in erythrocytes. We used hemolysis under different osmotic solutions to evaluate the fragility of erythrocytes. Data showed that MTE treatment dose-dependently increased the ratio of erythrocyte fragmentation （P〈0.001） and shrinking, and elevated the forward scatter （FSC） value （P〈0.001） and calcium accumulation （P〈0.001）. MTE induced ROS production of erythrocytes under the high glucose condition （P〈0.01） and consequently caused a rise in fragility （P〈0.05）. These results suggest that MTE induces cytotoxicity and aging in erythrocytes in a dose-dependent manner, and presents the possibility of impairment on cancer patients＇ circulating erythrocytes when MTE is used as an anti-cancer adjuvant medicine.展开更多
基金Supported by State Key Development Program for Basic Research of China(2007CB210208)National Science and Technology Major Project of China(2008ZX07101)China Scholarship Council(CSC),Natural Science and Engineering Research Council of Canada(NSERC),BIOCAP,and Canadian Funding for Innovations(CFI)
文摘Bio-oil is a new liquid fuel produced by fast pyrolysis,which is a promising technology to convert bio-mass into liquid. Pyrolytic lignin extracted from bio-oil,a fine powder,contributes to the instability of bio-oil. The paper presents the structural features of three kinds of pyrolytic lignin extracted from bio-oil with different methods(WIF,HMM,and LMM) . The pyrolytic lignin samples are characterized by Fourier transform infrared spectrometer(FTIR) and X-ray photoelectron spectroscopy(XPS) . FTIR data indicate that the three pyrolytic lignin samples have similar functional groups,while the absorption intensity is different,and show characteristic vibra-tions of typical lignocellulosic material groups O H(3340-3380 cm-1) ,C H(2912-2929 cm-1) and C O(1652-1725 cm-1) . Comparison in the region(3340-3380 cm-1) indicates that WIF has more O H stretch groups than HMM and LMM. The carbon spectra are fitted to four peaks:C1,C C or C H,BE 283.5 eV;C2,C OR or C OH,BE 284.5-285.8 eV;C3,C O or HO C OR,BE 286.10-287.10 eV;C4,O C O,BE 287.5-287.7 eV. The absence of C1,C C or C H indicates the dominant polymerization structure of aro-matic carbon in pyrolytic lignin samples. For HMM and WIF,C2a and C2b can not be separated,so there is no free hydroxyl group in the samples. The oxygen peaks are also fitted to four peaks:O1,OH,BE = 530.3 eV;O2,RC O,BE 531.45-531.72 eV;O3,O C O,BE = 532.73-533.74 eV;O4,H2O,BE 535 eV. The absence of O1 and O4 indicates that little hydroxyl groups and adsorbed water are present in the samples.
基金Project supported by the Zhejiang Provincial Natural Science Foundation(Nos.LQ16H070003,LY15H280010,and LY15C090004)the Key Project of Chinese Medicine in Zhejiang Province(No.2015ZZ001)+2 种基金the Traditional Chinese Medicine Scientific Research Foundation of Zhejiang Province(No.2014ZB007)the Traditional Chinese Medicine Outstanding Young Talent Foundation of Zhejiang Province(No.2014ZQ005)the Medicine and Health Research Foundation of Zhejiang Province(No.2015ZDA002),China
文摘Marsdeniae tenacissimae extract （MTE） has been used as an adjuvant medicine for cancer therapy for a long time. Although massive studies demonstrated its considerable anti-cancer effect, there is no research on its influence on erythrocytes, which are firstly interacted with MTE in the circulation. To investigate the influence of MTE on erythrocytes, we used a flow cytometer to detect the MTE-treated alternations of morphology, calcium concentration, and reactive oxygen species （ROS） level in erythrocytes. We used hemolysis under different osmotic solutions to evaluate the fragility of erythrocytes. Data showed that MTE treatment dose-dependently increased the ratio of erythrocyte fragmentation （P〈0.001） and shrinking, and elevated the forward scatter （FSC） value （P〈0.001） and calcium accumulation （P〈0.001）. MTE induced ROS production of erythrocytes under the high glucose condition （P〈0.01） and consequently caused a rise in fragility （P〈0.05）. These results suggest that MTE induces cytotoxicity and aging in erythrocytes in a dose-dependent manner, and presents the possibility of impairment on cancer patients＇ circulating erythrocytes when MTE is used as an anti-cancer adjuvant medicine.
