Cornus officinalis,a medicinal and edible plant known for its liver-nourishing properties,has shown promise in inhibiting the activation of hepatic stellate cells(HSCs),crucial indicators of hepatic fibrosis,especiall...Cornus officinalis,a medicinal and edible plant known for its liver-nourishing properties,has shown promise in inhibiting the activation of hepatic stellate cells(HSCs),crucial indicators of hepatic fibrosis,especially when processed by high pressure wine steaming(HPWS).Herein,this study aims to investigate the regulatory effects of cornus officinalis,both in its raw and HPWS forms,on inflammation and apoptosis in liver fibrosis and their underlying mechanisms.In vivo liver fibrosis models were established by subcutaneous injection of CCl4,while in vitro HSCs were exposed to transforming growth factor-b(TGF-b).These findings demonstrated that cornus officinalis with HPWS conspicuously ameliorated histopathological injury,reduced the release of proinflammatory factors,and decreased collagen deposition in CCl4-induced rats compared to its raw form.Utilizing ultra-high-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometer(UHPLC-QTOF-MS)combined with network analysis,we identified that the pharmacological effects of the changed components of cornus officinalis before and after HPWS,primarily centered on the adenosine phosphate(AMP)-activated protein kinase(AMPK)pathway.Of note,cornus officinalis activated AMPK and sirtuin 3(SIRT3),promoting the apoptosis of activated HSCs through the caspase cascade by regulating caspase3,caspase6 and caspase9.small interfering RNA(siRNA)experiments showed that cornus officinalis could regulate AMPK activity and its mediated-apoptosis through SIRT3.In conclusion,cornus officinalis exhibited the ability to reduce inflammation and apoptosis,with the SIRT3-AMPK signaling pathway identified as a potential mechanism underlying the synergistic effect of cornus officinalis with HPWS on anti-liver fibrosis.展开更多
Red osier dogwood(Cornus sericea L.),widely distributed throughout North America,is essential for wildlife,thus biodiversity.It is recommended for reclamation or revegetation of sites disturbed by oil and gas extracti...Red osier dogwood(Cornus sericea L.),widely distributed throughout North America,is essential for wildlife,thus biodiversity.It is recommended for reclamation or revegetation of sites disturbed by oil and gas extraction because it tolerates a wide range of soil types and high pH levels.Since germination of this species is extremely difficult with long stratification requirements and poor germination,cuttings facilitate propagation.In this study,to develop techniques to propagate the species from stem cuttings,four concentrations of indole-3-butyric acid(IBA)and two cutting ages previous year’s growth(PYG)and current year’s growth(CYG)were investigated for survival and growth.After 4 months,survival rate,height growth,and shoot biomass from PYG cuttings were enhanced with IB A.In contrast,IB A did not affect these parameters in CYG cuttings.Root morphology was significantly affected by IB A concentration and cutting age.IBA,at higher concentrations,increased root surface and length.Total root surface area and length of PYG cuttings were increased,which may facilitate the absorption of essential resources and consequently increase growth.The results indicate that increasing the concentration of IB A may be an effective way to have better plant survival and growth of previous year’s cuttings in red osier dogwood.展开更多
Objective: To investigate the mechanism of Cornus officinalis Total Glycosides (COTG) on myocardial protection, by studying effects of COTG on cardiomyocyte apoptosis induced by hypoxia/reoxygenation and calcium conce...Objective: To investigate the mechanism of Cornus officinalis Total Glycosides (COTG) on myocardial protection, by studying effects of COTG on cardiomyocyte apoptosis induced by hypoxia/reoxygenation and calcium concentration in rats. Methods: The myocardial cells of born 1-3d SD rats were isolated by enzyme digestion, cultured for 3 days. Cells were divided into five groups: Control group, H/R group, Cornus officinalis Total Glycosides low-dose group (LDG), Cornus officinalis Total Glycosides middle-dose group (MDG) and Cornus officinalis Total Glycosides high-dose group (HDG). Three drug groups were pretreated with different doses of Cornus officinalis Total Glycosides before hypoxia/reoxygenation treatment. The apoptotic rate was determined by flow cytometry assay, the intracellular free calcium concentration was examined by flow cytometry, and the ultrastructure of myocardial cells was observed under transmission electron microscope. Results: The results revealed that Cornus officinalis Total Glycosides pretreatment decreased apoptosis rate, but the effect of lower dosage is not significant. Furthermore, Cornus officinalis Total Glycosides can attenuate mitochondrial calcium overload, improve mitochondrial morphology and inhibit cardiomyocyte apoptosis caused by H/R. Conclusion: Cornus officinalis Total Glycosides pretreatment can inhibit cardiomyocyte apoptosis and calcium overload during H/R injury. However, the underlying mechanisms require us to further study.展开更多
Objective: To investigate effects of Cornus officinalis Total Glycosides (COTG) and Cornus Polysaccharide (CP) on myocardial protection and on expression of mitochondria biogenesis related gene of acute myocardial inf...Objective: To investigate effects of Cornus officinalis Total Glycosides (COTG) and Cornus Polysaccharide (CP) on myocardial protection and on expression of mitochondria biogenesis related gene of acute myocardial infarction (AMI) rats, Materials and Methods: Ninety-six SD rats of SPF level were randomly divided into 5 groups: sham operation group, model group, preventive treatment group, COTG treatment group, CP treatment group, and there were 12 cases in each one. By legating the left anterior descending branch of coronary artery method, acute myocardial infarction model was established. The rat of sham operation group and model group was intragastric administered with physiological saline;other groups were given with corresponding drugs. The cardiac function, the myocardial infarct area, the expression of mitochondrial biogenesis genes such as PGC-1α, PGC-1β, NRF-1mRNA and GSK-3β mRNA, GSK-3β Protein Expression were analyzed. Results: The results revealed that compared with model group, myocardial infarction size, LVDs, LVDd, LVESV, LVEDP, and -dp/dt decreased;LVSP increased in preventive treatment group, COTG treatment group, and CP treatment group (p < 0.05);LVEDV increased in preventive treatment group (p < 0.05), PGC 1 alpha, and PGC 1 beta;the NRF-1 mRNA expression increased in preventive treatment group, COTG treatment group, and CP treatment group (p < 0.05). Compared with CP and COTG treatment group, PGClpha, beta PGC 1, the NRF-1 mRNA expression increased in preventive treatment group (p < 0.05). Compared with the sham operation group, GSK-3 beta mRNA and protein expression increased in model group, preventive treatment group, COTG treatment group, and CP treatment group (p < 0.05). Compared with model group, GSK-3 beta mRNA expression reduced in preventive treatment group, COTG treatment group, and CP treatment group (p Cornus officinalis total glycosides and Cornus polysaccharides can effectively protect myocardial mitochondria of acute myocardial infarction rats by activating GSK-3β signaling pathways, and reduce the myocardial infarct size, which has great significance for improving cardiac function.展开更多
Arasbaran forests are located in East Azerbaijan （northwest Iran）. Increasing of socio-economic problems in this area causes destruction of biodiversity and structure of these forests. Using multipurpose trees such ...Arasbaran forests are located in East Azerbaijan （northwest Iran）. Increasing of socio-economic problems in this area causes destruction of biodiversity and structure of these forests. Using multipurpose trees such as cornelian cherry （Cornus mas L） to encourage villagers to produce forest by-products is a basic approach for preserving these forests. This species grows naturally in Arasbaran forests and the fruit is annually exploited using traditional harvest methods. This study aims to assess the ecological requirements of cornelian cherry and the important factors affecting its distribution. For this purpose, 40 circular sampling plots （300 m2） on various slope aspects were demarcated for sampling the occurrence of cornelian cherry in forest stands. DBH and crown cover percentage on north aspects were significantly greater than on other aspects and 4.5% of all trees were cornelian cherry in mature forest stands. North aspects had more seed-origin trees （standards） of cornelian cherry than coppiced trees, while west facing aspects had more coppiced than standard trees. This species had the highest regeneration rate in the sapling stage of 0–2.5 cm DBH. Thus, I recommend cultivation and development of cornelian cherry as a multi-purpose tree in the Arasbaran region on degraded forest lands on north and west aspects.展开更多
Cornelian cherry is used in the food and pharmaceutical industry as an ornament, in traditional medicine, and in the manufacture of household items. It is widely used in medicine for the prevention and treatment...Cornelian cherry is used in the food and pharmaceutical industry as an ornament, in traditional medicine, and in the manufacture of household items. It is widely used in medicine for the prevention and treatment of many diseases. Therefore, it is important to research the chemical composition of these species. In the article, based on our research, the analysis of the quantitative calculation of flavonoids and amino acids of the fruits of the “Elegant” and “Svetlyachok” cherry varieties was carried out. It was found that the amount of amino acids in the fruits of Cornus mas L., introduced in Uzbekistan, is higher in the variety “Svetlyachok” 2.643235 mg/g. In “Elegant” variety it was 1.794235 mg/g. The amount of 4 different flavonoids in the fruit was also determined. It has been established that the Elegant variety has a high concentration of lutein and rutin, and the svetlyachok has a high concentration of apigenin and quercetin.展开更多
Cornus wilsoniana fruit oil is a very important woody oil and is the main raw material of biodiesel.In this study,the oil yield,physicochemical properties,fatty acid composition,rheological properties,thermal stabilit...Cornus wilsoniana fruit oil is a very important woody oil and is the main raw material of biodiesel.In this study,the oil yield,physicochemical properties,fatty acid composition,rheological properties,thermal stability,and Fourier transform infrared(FTIR)spectra of C.wilsoniana fruit oil obtained by subcritical n-butane extraction(SBE)and conventional methods such as pressing extraction(PE)and Soxhlet extraction(SE)were determined to study the influence of different extraction methods on the quality and yield of C.wilsoniana fruit oil.The oil yield of SBE(19.47%)was higher than that of PE(9.93%)but slightly lower than that of SE(21.08%).All of the extracted oils exhibited similar physicochemical properties,and the SBE oil was richer in polyunsaturated fatty acids(PUFA)than that of the PE oil,with an approximate 1:2 ratio of total saturated fatty acids against unsaturated fatty acids.The results of rheological behavior and thermal stability showed that all extracted oils had Newtonian flow characteristics,wherein the SBE oil exhibited lower viscosity and higher thermal stability.Furthermore,scanning electron microscopy(SEM)images of the surface topography indicated that different oil extraction methods will affect the residual oil content of the C.wilsoniana fruit powder.Compared with PE,the pores on the surface of the C.wilsoniana fruit powder after oil extraction were clearly visible,indicating that the driving force of SBE for oil extraction is stronger than that of PE.Based on the above results,it is implied that SBE is the best of the three methods for extracting C.wilsoniana fruit oil and can be potentially applied to extract other edible oils.展开更多
基金supported by the National Natural Science Foundation of China(Grant Nos.:82104394 and 81973481)the Natural Science Foundation of Zhejiang Province(Grant Nos.:LY23H280008 and LQ23H280004)+2 种基金Zhejiang Province Traditional Chinese Medicine Science and Technology Project(Grant No.:2022ZQ033)Zhejiang Chinese Medicine University UniversityLevel Talent Special Project(Grant No.:2021ZR06)Zhejiang Province Postdoctoral Research Project(Grant No.:ZJ2022057).
文摘Cornus officinalis,a medicinal and edible plant known for its liver-nourishing properties,has shown promise in inhibiting the activation of hepatic stellate cells(HSCs),crucial indicators of hepatic fibrosis,especially when processed by high pressure wine steaming(HPWS).Herein,this study aims to investigate the regulatory effects of cornus officinalis,both in its raw and HPWS forms,on inflammation and apoptosis in liver fibrosis and their underlying mechanisms.In vivo liver fibrosis models were established by subcutaneous injection of CCl4,while in vitro HSCs were exposed to transforming growth factor-b(TGF-b).These findings demonstrated that cornus officinalis with HPWS conspicuously ameliorated histopathological injury,reduced the release of proinflammatory factors,and decreased collagen deposition in CCl4-induced rats compared to its raw form.Utilizing ultra-high-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometer(UHPLC-QTOF-MS)combined with network analysis,we identified that the pharmacological effects of the changed components of cornus officinalis before and after HPWS,primarily centered on the adenosine phosphate(AMP)-activated protein kinase(AMPK)pathway.Of note,cornus officinalis activated AMPK and sirtuin 3(SIRT3),promoting the apoptosis of activated HSCs through the caspase cascade by regulating caspase3,caspase6 and caspase9.small interfering RNA(siRNA)experiments showed that cornus officinalis could regulate AMPK activity and its mediated-apoptosis through SIRT3.In conclusion,cornus officinalis exhibited the ability to reduce inflammation and apoptosis,with the SIRT3-AMPK signaling pathway identified as a potential mechanism underlying the synergistic effect of cornus officinalis with HPWS on anti-liver fibrosis.
基金supported by the Natural Sciences and Engineering Research Council of Canada(Innovation Enhancement Grants—NSERC CCIP 517845-17)。
文摘Red osier dogwood(Cornus sericea L.),widely distributed throughout North America,is essential for wildlife,thus biodiversity.It is recommended for reclamation or revegetation of sites disturbed by oil and gas extraction because it tolerates a wide range of soil types and high pH levels.Since germination of this species is extremely difficult with long stratification requirements and poor germination,cuttings facilitate propagation.In this study,to develop techniques to propagate the species from stem cuttings,four concentrations of indole-3-butyric acid(IBA)and two cutting ages previous year’s growth(PYG)and current year’s growth(CYG)were investigated for survival and growth.After 4 months,survival rate,height growth,and shoot biomass from PYG cuttings were enhanced with IB A.In contrast,IB A did not affect these parameters in CYG cuttings.Root morphology was significantly affected by IB A concentration and cutting age.IBA,at higher concentrations,increased root surface and length.Total root surface area and length of PYG cuttings were increased,which may facilitate the absorption of essential resources and consequently increase growth.The results indicate that increasing the concentration of IB A may be an effective way to have better plant survival and growth of previous year’s cuttings in red osier dogwood.
文摘Objective: To investigate the mechanism of Cornus officinalis Total Glycosides (COTG) on myocardial protection, by studying effects of COTG on cardiomyocyte apoptosis induced by hypoxia/reoxygenation and calcium concentration in rats. Methods: The myocardial cells of born 1-3d SD rats were isolated by enzyme digestion, cultured for 3 days. Cells were divided into five groups: Control group, H/R group, Cornus officinalis Total Glycosides low-dose group (LDG), Cornus officinalis Total Glycosides middle-dose group (MDG) and Cornus officinalis Total Glycosides high-dose group (HDG). Three drug groups were pretreated with different doses of Cornus officinalis Total Glycosides before hypoxia/reoxygenation treatment. The apoptotic rate was determined by flow cytometry assay, the intracellular free calcium concentration was examined by flow cytometry, and the ultrastructure of myocardial cells was observed under transmission electron microscope. Results: The results revealed that Cornus officinalis Total Glycosides pretreatment decreased apoptosis rate, but the effect of lower dosage is not significant. Furthermore, Cornus officinalis Total Glycosides can attenuate mitochondrial calcium overload, improve mitochondrial morphology and inhibit cardiomyocyte apoptosis caused by H/R. Conclusion: Cornus officinalis Total Glycosides pretreatment can inhibit cardiomyocyte apoptosis and calcium overload during H/R injury. However, the underlying mechanisms require us to further study.
文摘Objective: To investigate effects of Cornus officinalis Total Glycosides (COTG) and Cornus Polysaccharide (CP) on myocardial protection and on expression of mitochondria biogenesis related gene of acute myocardial infarction (AMI) rats, Materials and Methods: Ninety-six SD rats of SPF level were randomly divided into 5 groups: sham operation group, model group, preventive treatment group, COTG treatment group, CP treatment group, and there were 12 cases in each one. By legating the left anterior descending branch of coronary artery method, acute myocardial infarction model was established. The rat of sham operation group and model group was intragastric administered with physiological saline;other groups were given with corresponding drugs. The cardiac function, the myocardial infarct area, the expression of mitochondrial biogenesis genes such as PGC-1α, PGC-1β, NRF-1mRNA and GSK-3β mRNA, GSK-3β Protein Expression were analyzed. Results: The results revealed that compared with model group, myocardial infarction size, LVDs, LVDd, LVESV, LVEDP, and -dp/dt decreased;LVSP increased in preventive treatment group, COTG treatment group, and CP treatment group (p < 0.05);LVEDV increased in preventive treatment group (p < 0.05), PGC 1 alpha, and PGC 1 beta;the NRF-1 mRNA expression increased in preventive treatment group, COTG treatment group, and CP treatment group (p < 0.05). Compared with CP and COTG treatment group, PGClpha, beta PGC 1, the NRF-1 mRNA expression increased in preventive treatment group (p < 0.05). Compared with the sham operation group, GSK-3 beta mRNA and protein expression increased in model group, preventive treatment group, COTG treatment group, and CP treatment group (p < 0.05). Compared with model group, GSK-3 beta mRNA expression reduced in preventive treatment group, COTG treatment group, and CP treatment group (p Cornus officinalis total glycosides and Cornus polysaccharides can effectively protect myocardial mitochondria of acute myocardial infarction rats by activating GSK-3β signaling pathways, and reduce the myocardial infarct size, which has great significance for improving cardiac function.
文摘Arasbaran forests are located in East Azerbaijan （northwest Iran）. Increasing of socio-economic problems in this area causes destruction of biodiversity and structure of these forests. Using multipurpose trees such as cornelian cherry （Cornus mas L） to encourage villagers to produce forest by-products is a basic approach for preserving these forests. This species grows naturally in Arasbaran forests and the fruit is annually exploited using traditional harvest methods. This study aims to assess the ecological requirements of cornelian cherry and the important factors affecting its distribution. For this purpose, 40 circular sampling plots （300 m2） on various slope aspects were demarcated for sampling the occurrence of cornelian cherry in forest stands. DBH and crown cover percentage on north aspects were significantly greater than on other aspects and 4.5% of all trees were cornelian cherry in mature forest stands. North aspects had more seed-origin trees （standards） of cornelian cherry than coppiced trees, while west facing aspects had more coppiced than standard trees. This species had the highest regeneration rate in the sapling stage of 0–2.5 cm DBH. Thus, I recommend cultivation and development of cornelian cherry as a multi-purpose tree in the Arasbaran region on degraded forest lands on north and west aspects.
文摘Cornelian cherry is used in the food and pharmaceutical industry as an ornament, in traditional medicine, and in the manufacture of household items. It is widely used in medicine for the prevention and treatment of many diseases. Therefore, it is important to research the chemical composition of these species. In the article, based on our research, the analysis of the quantitative calculation of flavonoids and amino acids of the fruits of the “Elegant” and “Svetlyachok” cherry varieties was carried out. It was found that the amount of amino acids in the fruits of Cornus mas L., introduced in Uzbekistan, is higher in the variety “Svetlyachok” 2.643235 mg/g. In “Elegant” variety it was 1.794235 mg/g. The amount of 4 different flavonoids in the fruit was also determined. It has been established that the Elegant variety has a high concentration of lutein and rutin, and the svetlyachok has a high concentration of apigenin and quercetin.
基金the National Key Research and Development Projects of China(2019Y FB1504001)for the financial supports of this research
文摘Cornus wilsoniana fruit oil is a very important woody oil and is the main raw material of biodiesel.In this study,the oil yield,physicochemical properties,fatty acid composition,rheological properties,thermal stability,and Fourier transform infrared(FTIR)spectra of C.wilsoniana fruit oil obtained by subcritical n-butane extraction(SBE)and conventional methods such as pressing extraction(PE)and Soxhlet extraction(SE)were determined to study the influence of different extraction methods on the quality and yield of C.wilsoniana fruit oil.The oil yield of SBE(19.47%)was higher than that of PE(9.93%)but slightly lower than that of SE(21.08%).All of the extracted oils exhibited similar physicochemical properties,and the SBE oil was richer in polyunsaturated fatty acids(PUFA)than that of the PE oil,with an approximate 1:2 ratio of total saturated fatty acids against unsaturated fatty acids.The results of rheological behavior and thermal stability showed that all extracted oils had Newtonian flow characteristics,wherein the SBE oil exhibited lower viscosity and higher thermal stability.Furthermore,scanning electron microscopy(SEM)images of the surface topography indicated that different oil extraction methods will affect the residual oil content of the C.wilsoniana fruit powder.Compared with PE,the pores on the surface of the C.wilsoniana fruit powder after oil extraction were clearly visible,indicating that the driving force of SBE for oil extraction is stronger than that of PE.Based on the above results,it is implied that SBE is the best of the three methods for extracting C.wilsoniana fruit oil and can be potentially applied to extract other edible oils.
