Using electron spin resonance (ESR) spin labeling technique, we have studied the conformation of sulfhydryl groups(-SH) binding sites in membrane proteins and membrane fluidity of red blood cells (RBCs) from two group...Using electron spin resonance (ESR) spin labeling technique, we have studied the conformation of sulfhydryl groups(-SH) binding sites in membrane proteins and membrane fluidity of red blood cells (RBCs) from two groups of patients with anemia of chronic renal failure (ACRF). One of the groups is composed of patients who were untreated with recombinant human erythropoietin(r-HuEPO), and the other is composed of patients who were treated with r-HuEPO. The results indicated: 1) the conformation of SH group binding site in RBC membrane proteins from former group was different from those of health people. 2)the fluidity in the region near the surface of RBC membrane from former group was lower than those of healthy people. 3) However, the above biophysical properties of RBC membrane from later group were normal. We concluded that RBC membrane in patients with ACRF was abnormal, and the treatment of r-HuEPO may promote the production of normal RBCs, thus ameliorate the biophysical properties of RBCs from the patients with ACRF.展开更多
In this paper,we introduce our finding of the effects of C_(60) nanoparticles (NP) infiltration on mechanical properties of cell and its membrane.Atomic force microscopy (AFM) is used to perform indentation on both no...In this paper,we introduce our finding of the effects of C_(60) nanoparticles (NP) infiltration on mechanical properties of cell and its membrane.Atomic force microscopy (AFM) is used to perform indentation on both normal and C_(60) infiltrated red blood cells (RBC) to gain data of mechanical characteristics of the membrane.Our results show that the mechanical properties of human RBC membrane seem to be altered due to the presence of C_(60) NPs.The resistance and ultimate strength of the C_(60) infiltrated RBC membrane significantly decrease.We also explain the mechanism of how C_(60) NPs infiltration changes the mechanical properties of the cell membrane by predicting the structural change of the lipid bilayer caused by the C_(60) infiltration at molecular level and analyze the interactions among molecules in the lipid bilayer.The potential hazards and application of the change in mechanical characteristics of the RBCs membrane are also discussed.Nanotoxicity of C_(60) NPs may be significant for some biological cells.展开更多
文摘Using electron spin resonance (ESR) spin labeling technique, we have studied the conformation of sulfhydryl groups(-SH) binding sites in membrane proteins and membrane fluidity of red blood cells (RBCs) from two groups of patients with anemia of chronic renal failure (ACRF). One of the groups is composed of patients who were untreated with recombinant human erythropoietin(r-HuEPO), and the other is composed of patients who were treated with r-HuEPO. The results indicated: 1) the conformation of SH group binding site in RBC membrane proteins from former group was different from those of health people. 2)the fluidity in the region near the surface of RBC membrane from former group was lower than those of healthy people. 3) However, the above biophysical properties of RBC membrane from later group were normal. We concluded that RBC membrane in patients with ACRF was abnormal, and the treatment of r-HuEPO may promote the production of normal RBCs, thus ameliorate the biophysical properties of RBCs from the patients with ACRF.
基金supported by the National Natural Science Foundation of China (Grant Nos.10902128,11072271,10972239,51172291)Fundamental Research Funds for the Central Universities,New Century Excellent Talents in University and Research Funds for the Doctoral Program of Higher Education
文摘In this paper,we introduce our finding of the effects of C_(60) nanoparticles (NP) infiltration on mechanical properties of cell and its membrane.Atomic force microscopy (AFM) is used to perform indentation on both normal and C_(60) infiltrated red blood cells (RBC) to gain data of mechanical characteristics of the membrane.Our results show that the mechanical properties of human RBC membrane seem to be altered due to the presence of C_(60) NPs.The resistance and ultimate strength of the C_(60) infiltrated RBC membrane significantly decrease.We also explain the mechanism of how C_(60) NPs infiltration changes the mechanical properties of the cell membrane by predicting the structural change of the lipid bilayer caused by the C_(60) infiltration at molecular level and analyze the interactions among molecules in the lipid bilayer.The potential hazards and application of the change in mechanical characteristics of the RBCs membrane are also discussed.Nanotoxicity of C_(60) NPs may be significant for some biological cells.
