A total of 142 specimens of Ceramiales （Rhodophyta） were collected each month from October 2011 to November 2012 in the intertidal zone of the northwestern Yellow Sea. These specimens covered 21 species, 14 genera, ...A total of 142 specimens of Ceramiales （Rhodophyta） were collected each month from October 2011 to November 2012 in the intertidal zone of the northwestern Yellow Sea. These specimens covered 21 species, 14 genera, and four families. Cluster analyses show that the specimens had a high diversity for the three DNA markers, namely, partial large subunit rRNA gene （LSU）, universal plastid amplicon （UPA）, and partial mitochondrial cytochrome c oxidase subunit I gene （COl）. No intraspecific divergence was found in our collection for these markers, except for a 1-3 bp divergence in the COI of Ceramium kondoi, Syrnphyocladia latiuscula, and Neosiphoniajaponica. Because short DNA markers were used, the phylogenetic relationships of higher taxonomic levels were hard to evaluate with poor branch support. More than half species of our collection failed to find their matched sequences owing to shortage information of DNA barcodes for macroalgae in GenBank or BOLD （Barcode of Life Data） Systems. Three specimens were presumed as Heterosiphonia crispella by cluster analyses on DNA barcodes assisted by morphological identification, which was the first record in the investigated area, implying that it might he a cryptic or invasive species in the coastal area of northwestern Yellow Sea. In the neighbor-joining trees of all three DNA markers, Heterosiphonia japonica converged with Dasya spp. and was distant from the other Heterosiphonia spp., implying that H.japonica had affinities to the genus Dasya. The LSU and UPA markers amplified and sequenced easier than the COI marker across the Ceramiales species, but the COI had a higher ability to discriminate between species.展开更多
Anthocyanin is a water soluble natural pigmen, which could be extracted from Red Rose. Color is an important factor to determine the quality of foods, so that the pigments isolation must be selected type of solvent fo...Anthocyanin is a water soluble natural pigmen, which could be extracted from Red Rose. Color is an important factor to determine the quality of foods, so that the pigments isolation must be selected type of solvent for extraction process. Besides functioning as dyes, anthocyanins have a role as a good antioxidant, so it can be used to protect the fat content of fermented milk such as yoghurt. This experiment was to find out the effect of different solvent type on extraction pigment （aquadest： sitrat acid/lactic acid/sulfate acid/chloride acid） and to determine the effect of the addition of anthocyanins in protecting the fat content of yogurt on several levels addition. The research result showed that anthocyanin＇s pigment of red rose from petal flower＇s could be effective solvent extracted using lactic acid 0.02 M and increased fat stability of yoghurt. The anthocyanin pigment showed with HPLC analysis that pigment resulted TLC analysis at rite time 13.10 （as Cyanidin glucoside） and 22.61 （as Malvidin glucoside）. Yoghurt that given anthocyanin pigment has proven that fat content can be maintained its 86.7% （fat content with 2% pigment as such as 0.117% and without pigment 0.087%） after being stored for 6 days at cold and room temperature.展开更多
Rhodopseudomonas palustris, one of purple nonsulfur photosynthetic bacteria, fixes carbon dioxide via Calvin-Benson cycle and has been shown previously to express form I and form II ribulose-1,5-bisphosphate carboxyla...Rhodopseudomonas palustris, one of purple nonsulfur photosynthetic bacteria, fixes carbon dioxide via Calvin-Benson cycle and has been shown previously to express form I and form II ribulose-1,5-bisphosphate carboxylase/oxygenase (RubisCO). The gene cbbM, which encodes the form II enzyme from Rhodopseudomonas palustris, has been subcloned and sequenced. The deduced amino acid sequence is highly with the form II RubisCO from photosynthetic bacteria, including Rhodospirillum rubrum (PDB ID: 9rub), but appears to be more distantly related to the large subunit of the form I RubisCO found in photosynthetic bacteria, chemoautotrophic bacteria and higher plants. Several regions highly conserved among L 8S 8 and L x enzymes correspond with regions previously implicated in catalytic activity and subunit interactions.展开更多
In this paper,we demonstrate the conversion of graphene oxide(GO) into boron carbon oxynitride(BCNO) hybrid nanosheets via a reaction with boric acid and urea,during which the boron and nitrogen atoms are incorporated...In this paper,we demonstrate the conversion of graphene oxide(GO) into boron carbon oxynitride(BCNO) hybrid nanosheets via a reaction with boric acid and urea,during which the boron and nitrogen atoms are incorporated into graphene nanosheets.The experimental results reveal that GO is important for the photoluminescence(PL) BCNO phosphor particles.More importantly,in this system,the prepared BCNO phosphors can be used to prepare the materials needed for red light emitting diodes(LEDs).展开更多
基金Supported by the Public Science and Technology Research Funds Projects of Ocean(Nos.201105021,201305030)the National Natural Science Foundation of China(No.41276137)
文摘A total of 142 specimens of Ceramiales （Rhodophyta） were collected each month from October 2011 to November 2012 in the intertidal zone of the northwestern Yellow Sea. These specimens covered 21 species, 14 genera, and four families. Cluster analyses show that the specimens had a high diversity for the three DNA markers, namely, partial large subunit rRNA gene （LSU）, universal plastid amplicon （UPA）, and partial mitochondrial cytochrome c oxidase subunit I gene （COl）. No intraspecific divergence was found in our collection for these markers, except for a 1-3 bp divergence in the COI of Ceramium kondoi, Syrnphyocladia latiuscula, and Neosiphoniajaponica. Because short DNA markers were used, the phylogenetic relationships of higher taxonomic levels were hard to evaluate with poor branch support. More than half species of our collection failed to find their matched sequences owing to shortage information of DNA barcodes for macroalgae in GenBank or BOLD （Barcode of Life Data） Systems. Three specimens were presumed as Heterosiphonia crispella by cluster analyses on DNA barcodes assisted by morphological identification, which was the first record in the investigated area, implying that it might he a cryptic or invasive species in the coastal area of northwestern Yellow Sea. In the neighbor-joining trees of all three DNA markers, Heterosiphonia japonica converged with Dasya spp. and was distant from the other Heterosiphonia spp., implying that H.japonica had affinities to the genus Dasya. The LSU and UPA markers amplified and sequenced easier than the COI marker across the Ceramiales species, but the COI had a higher ability to discriminate between species.
文摘Anthocyanin is a water soluble natural pigmen, which could be extracted from Red Rose. Color is an important factor to determine the quality of foods, so that the pigments isolation must be selected type of solvent for extraction process. Besides functioning as dyes, anthocyanins have a role as a good antioxidant, so it can be used to protect the fat content of fermented milk such as yoghurt. This experiment was to find out the effect of different solvent type on extraction pigment （aquadest： sitrat acid/lactic acid/sulfate acid/chloride acid） and to determine the effect of the addition of anthocyanins in protecting the fat content of yogurt on several levels addition. The research result showed that anthocyanin＇s pigment of red rose from petal flower＇s could be effective solvent extracted using lactic acid 0.02 M and increased fat stability of yoghurt. The anthocyanin pigment showed with HPLC analysis that pigment resulted TLC analysis at rite time 13.10 （as Cyanidin glucoside） and 22.61 （as Malvidin glucoside）. Yoghurt that given anthocyanin pigment has proven that fat content can be maintained its 86.7% （fat content with 2% pigment as such as 0.117% and without pigment 0.087%） after being stored for 6 days at cold and room temperature.
文摘Rhodopseudomonas palustris, one of purple nonsulfur photosynthetic bacteria, fixes carbon dioxide via Calvin-Benson cycle and has been shown previously to express form I and form II ribulose-1,5-bisphosphate carboxylase/oxygenase (RubisCO). The gene cbbM, which encodes the form II enzyme from Rhodopseudomonas palustris, has been subcloned and sequenced. The deduced amino acid sequence is highly with the form II RubisCO from photosynthetic bacteria, including Rhodospirillum rubrum (PDB ID: 9rub), but appears to be more distantly related to the large subunit of the form I RubisCO found in photosynthetic bacteria, chemoautotrophic bacteria and higher plants. Several regions highly conserved among L 8S 8 and L x enzymes correspond with regions previously implicated in catalytic activity and subunit interactions.
基金supported by the National Natural Science Foundation of China(No.11404397)
文摘In this paper,we demonstrate the conversion of graphene oxide(GO) into boron carbon oxynitride(BCNO) hybrid nanosheets via a reaction with boric acid and urea,during which the boron and nitrogen atoms are incorporated into graphene nanosheets.The experimental results reveal that GO is important for the photoluminescence(PL) BCNO phosphor particles.More importantly,in this system,the prepared BCNO phosphors can be used to prepare the materials needed for red light emitting diodes(LEDs).
