[Objective] This study aimed to analyze the genetic relationship of the Carthamus tinctorius L（safflower） varieties in Xinjiang, and thus to provide a scientific basis for its protection and development. [Method] Th...[Objective] This study aimed to analyze the genetic relationship of the Carthamus tinctorius L（safflower） varieties in Xinjiang, and thus to provide a scientific basis for its protection and development. [Method] The genomic DNA of five safflower varieties from Xinjiang and one from Yunnan were analyzed and compared by SRAP molecular marker. [Result] Twelve pairs of SRAP primers were selected to amplify the genomic DNA of the six materials. Among the 171 clear DNA bands finally obtained, 93 were polymorphic, accounting for 54.4% of total. Genetic similarity coefficient of the six safflower varieties ranged from 0.60 to 0.92. [Conclusion]SRAP molecular marker is suitable for safflower varieties research and to guide the molecular breeding.展开更多
Anthocyanin is a water soluble natural pigmen, which could be extracted from Red Rose. Color is an important factor to determine the quality of foods, so that the pigments isolation must be selected type of solvent fo...Anthocyanin is a water soluble natural pigmen, which could be extracted from Red Rose. Color is an important factor to determine the quality of foods, so that the pigments isolation must be selected type of solvent for extraction process. Besides functioning as dyes, anthocyanins have a role as a good antioxidant, so it can be used to protect the fat content of fermented milk such as yoghurt. This experiment was to find out the effect of different solvent type on extraction pigment （aquadest： sitrat acid/lactic acid/sulfate acid/chloride acid） and to determine the effect of the addition of anthocyanins in protecting the fat content of yogurt on several levels addition. The research result showed that anthocyanin＇s pigment of red rose from petal flower＇s could be effective solvent extracted using lactic acid 0.02 M and increased fat stability of yoghurt. The anthocyanin pigment showed with HPLC analysis that pigment resulted TLC analysis at rite time 13.10 （as Cyanidin glucoside） and 22.61 （as Malvidin glucoside）. Yoghurt that given anthocyanin pigment has proven that fat content can be maintained its 86.7% （fat content with 2% pigment as such as 0.117% and without pigment 0.087%） after being stored for 6 days at cold and room temperature.展开更多
A quick and sensitive method was developed for the determination of 19 metal elements in Saffron,a traditional Chinese medicinal herb,collected from Tibet Autonomous Region and Henan Province of China using inductivel...A quick and sensitive method was developed for the determination of 19 metal elements in Saffron,a traditional Chinese medicinal herb,collected from Tibet Autonomous Region and Henan Province of China using inductively coupled plasma atomic emission spectrometry（ICP-AES）or inductively coupled plasma mass spectrometry（ICP-MS）.The determined 19 metals in Saffron include Ca,Fe,Mg,P,Sr,Al,Mn,Zn,V,Cr,Se,Co,Ni,Mo,and heavy metals As,Cu,Cd,Hg and Pb.For all the analyzed elements,the correlative coefficients of the calibration curves were no less than 0.9938.This proposed method was accurate,and the relative standard deviations of the measurements were lower than 5.25%.It can be used for the quality control of metal elements in Saffron.展开更多
基金Supported by Natural Science Foundation of Hubei Province(2010CDZ011)National Project for College Students to Start Innovative Career(GCX12014)~~
文摘[Objective] This study aimed to analyze the genetic relationship of the Carthamus tinctorius L（safflower） varieties in Xinjiang, and thus to provide a scientific basis for its protection and development. [Method] The genomic DNA of five safflower varieties from Xinjiang and one from Yunnan were analyzed and compared by SRAP molecular marker. [Result] Twelve pairs of SRAP primers were selected to amplify the genomic DNA of the six materials. Among the 171 clear DNA bands finally obtained, 93 were polymorphic, accounting for 54.4% of total. Genetic similarity coefficient of the six safflower varieties ranged from 0.60 to 0.92. [Conclusion]SRAP molecular marker is suitable for safflower varieties research and to guide the molecular breeding.
文摘Anthocyanin is a water soluble natural pigmen, which could be extracted from Red Rose. Color is an important factor to determine the quality of foods, so that the pigments isolation must be selected type of solvent for extraction process. Besides functioning as dyes, anthocyanins have a role as a good antioxidant, so it can be used to protect the fat content of fermented milk such as yoghurt. This experiment was to find out the effect of different solvent type on extraction pigment （aquadest： sitrat acid/lactic acid/sulfate acid/chloride acid） and to determine the effect of the addition of anthocyanins in protecting the fat content of yogurt on several levels addition. The research result showed that anthocyanin＇s pigment of red rose from petal flower＇s could be effective solvent extracted using lactic acid 0.02 M and increased fat stability of yoghurt. The anthocyanin pigment showed with HPLC analysis that pigment resulted TLC analysis at rite time 13.10 （as Cyanidin glucoside） and 22.61 （as Malvidin glucoside）. Yoghurt that given anthocyanin pigment has proven that fat content can be maintained its 86.7% （fat content with 2% pigment as such as 0.117% and without pigment 0.087%） after being stored for 6 days at cold and room temperature.
文摘A quick and sensitive method was developed for the determination of 19 metal elements in Saffron,a traditional Chinese medicinal herb,collected from Tibet Autonomous Region and Henan Province of China using inductively coupled plasma atomic emission spectrometry（ICP-AES）or inductively coupled plasma mass spectrometry（ICP-MS）.The determined 19 metals in Saffron include Ca,Fe,Mg,P,Sr,Al,Mn,Zn,V,Cr,Se,Co,Ni,Mo,and heavy metals As,Cu,Cd,Hg and Pb.For all the analyzed elements,the correlative coefficients of the calibration curves were no less than 0.9938.This proposed method was accurate,and the relative standard deviations of the measurements were lower than 5.25%.It can be used for the quality control of metal elements in Saffron.
