Aim To study the proliferative effeet of hydroxysaftlor yellow A （HSYA） on cultured canine aortic endothelial cell （VEC） in normoxic （21% O2 ） or hypoxic （10% O2 ） culture and the underlying mechanism. Methods...Aim To study the proliferative effeet of hydroxysaftlor yellow A （HSYA） on cultured canine aortic endothelial cell （VEC） in normoxic （21% O2 ） or hypoxic （10% O2 ） culture and the underlying mechanism. Methods The endothelial cells were scratched from trypsined canine aorta endothelium. HSYA was added to the cells at final concentrations of 1 × 10^-3, 1 × 10^-4 and 1 × 10^-5 mol· L^-1, respectively. VEGF （2.6 × 10^-7 mol· L^-1 ）-treated cells were used as the positive control. The proliferative effect of HSYA on VEC was determined at 48, 72, 96, and 120 h in normoxic culture by MTI＂ assay. Similarly, the proliferation of VEC was determined at 12, 24, 48, and 72 h in hypoxic culture by MTF assay. The effects of HSYA on VEC proliferation and VEGF secretion were investigated by MTr and ELISA assays at the presence of the antibodies to VEGF and VEGF receptors. Results Pretreatment with HSYA at concentrations of 1 × 10^-3 and 1 × 10^-4 mol· L^-1 enhanced VEC proliferation in normoxic culture. The most significant enhancing effect of HSYA on VEC proliferation was achieved at 24, 48, and 72 h in hypoxic culture in concentration-dependent and time-dependent manner. HSYA at 1 × 10^-3 mol·L^-1 showed a potency similar to VEGF at 2.6 × 10^-7 mol·L^-1 . Pretreatment with the antibodies of Flt-1, KDR or VEGF blocked the proliferative effect of HSYA with similar potencies. Antibodies of Fit-1 or VEGF antagonized the promoting effect of HSYA on VEGF secretion. Conclusion HSYA promotes VEC proliferation either in normoxic or hypoxic culture, especially in the latter condition. This effect of HSYA is at least partly mediated by VEGF and VEGF receptor.展开更多
目的:研究红花黄色素对神经根型腰椎病大鼠抗炎作用及其机制。方法:建立神经根型腰椎病大鼠模型,随机分为假手术组、模型组、PDTC(吡咯烷二硫代甲酸铵盐)组、红花黄色素100 mg/kg、50 mg/kg、25 mg/kg剂量组。红花黄色素各组于造模后3 ...目的:研究红花黄色素对神经根型腰椎病大鼠抗炎作用及其机制。方法:建立神经根型腰椎病大鼠模型,随机分为假手术组、模型组、PDTC(吡咯烷二硫代甲酸铵盐)组、红花黄色素100 mg/kg、50 mg/kg、25 mg/kg剂量组。红花黄色素各组于造模后3 d静脉注射给药,共给药7 d。检测后肢热痛刺激缩爪阈值的变化;椎板法检测血液流变学指标;Elisa法检测大鼠血清中TNF-α、IL-1β、IL-6、COX-2水平;Western法检测脊髓背角中p-NF-k B P65蛋白的表达;RT-PCR法检测NF-k B P65 mRNA的表达。结果:与模型组相比,100 mg/kg、50 mg/kg、25 mg/kg剂量的红花黄色素均能显著提高热痛刺激缩爪阈值、改善血液流变学各指标;显著降低TNF-α、IL-1β、IL-6、COX-2水平;显著抑制p-NF-k B P65蛋白及NF-k B P65 mRNA的表达。结论:红花黄色素可能对神经根型腰椎病的治疗有一定的意义,其机制与抑制NF-k B P65通路的激活有关。展开更多
Safflower is a popular Chinese medicinal plant and Safflower injection is extensively used for the clinical treatment of cerebrovascular and cardiovascular diseases. In this study, HPLC-DAD-ESI-MSn was utilized to stu...Safflower is a popular Chinese medicinal plant and Safflower injection is extensively used for the clinical treatment of cerebrovascular and cardiovascular diseases. In this study, HPLC-DAD-ESI-MSn was utilized to study the stability and degradation of the two major but chemically unstable bioactive compounds hydroxysaffior yellow A and anhydrosaffior yellow B, in Safflower injection. The impact of light irradiation, temperature, and pH on the stability of these two compounds were studied. The results showed that hydroxysafflor yellow A and anhydrosafflor yellow B could degrade at high temperature （〉60 ℃） or extreme pHs （pH ≤ 3.0 or 〉7.0）, but not under light irradiation. The common degradation product was p-coumaric acid. Chemical structures of the other degradation products were characterized by LC-MS. Hypothetical degradation pathways were proposed. In addition, ADP-induced platelet aggregation tests showed that the degradation of anhydrosaffior yellow B could reduce the anticoagulation activities of Safflower injection. Our results suggest that temperature and pH are critically important for the preparation and storage of Safflower injection.展开更多
OBJECTIVE:To study the effects of hydroxy safflower yellow A(HSYA) on tumor capillary angiogenesis in transplanted human gastric adenocarcinoma BGC-823 tumors in nude mice.METHODS:BGC-823 cells were injected subcutane...OBJECTIVE:To study the effects of hydroxy safflower yellow A(HSYA) on tumor capillary angiogenesis in transplanted human gastric adenocarcinoma BGC-823 tumors in nude mice.METHODS:BGC-823 cells were injected subcutaneously into the right anterior armpit of nude mice to establish an animal model of transplanted tumors.After 24 h,18 nude mice injected with tumor cells were randomized into model,control,and HSYA 0.028 g/L groups,with six mice in each group.Transplanted tumors were excised on day 20.Tumor inhibition ratios were calculated for the transplanted tumors.Pathological changes and capillary angiogenesis in the tumors were observed by light microscopy.RESULTS:Tumors in the model group grew more quickly than those in the control and HSYA groups,with inhibition ratios of 48% and 30%,respectively.The microvessel count in the HSYA group was lower than in the model group(P<0.01),and microvessel density was also lower in the HSYA group(P<0.05).Pathological changes were more obvious in tumors in the model group compared to the HSYA group.CONCLUSION:HSYA inhibits the growth of transplanted BGC-823 tumors,and its effects on tumor capillary angiogenesis may represent one of the mechanisms responsible for this antineoplastic effect.展开更多
OBJECTIVE： To evaluate the clinical efficacy of safflower yellow injection combined with conventional therapy in treating unstable angina pectoris.METHODS： We searched online databases： Chinese journal full-text da...OBJECTIVE： To evaluate the clinical efficacy of safflower yellow injection combined with conventional therapy in treating unstable angina pectoris.METHODS： We searched online databases： Chinese journal full-text database, China National Knowledge Infrastructure, Wanfang database, Chinese journal full-text database, Pubmed, ScienceDirect,Embase, and the Cochrane Library with manual-screening of relevant literature. Eligible randomized controlled trials（RCT） on angina pectoris were included. We conducted meta-analysis using the RevMan 5.1 software from The Cochrane Collaboration. We treated the relief rate of angina symptoms and electrocardiograph（ECG） as evaluation.RESULTS： Seven articles, including in 1134 patients, were enrolled after the evaluation. Therewas no significant heterogeneity among the studies（χ2=1.08, df=6, P=0.98, I2=0%）. The safflower yellow injection with conventional therapy has a higher effective rate than the control group in relieving the symptoms of angina pectoris [odds ratio（OR）=2.95, 95%（CI）（1.81, 4.81）] and improving ischemic ECG [OR=2.85, 95% CI（1.67, 4.86）]. The difference was statistically significant in the ＂80 mg dosage＂ and ＂100 mg dosage＂ subgroups（P0.05） for improving clinical symptoms and ECG. The funnel graphic was nearly symmetrical. Sensitivity analysis suggested that the results were stable.CONCLUSION： Safflower yellow injection as an adjunct therapy with conventional drugs shows advantages in easing the clinical symptoms of unstable angina and improving ECG over basic therapy alone. However, the conclusions should be interpreted with care until more high-quality RCTs are reported.展开更多
As an effective component of safflower,hydroxysafflor yellow A(HSYA)has the effect of promoting blood circulation,removing blood stasis,and relieving pain,and it has a certain effect on blood stasis and wind-induced P...As an effective component of safflower,hydroxysafflor yellow A(HSYA)has the effect of promoting blood circulation,removing blood stasis,and relieving pain,and it has a certain effect on blood stasis and wind-induced Parkinson‘s disease(PD).However,the current research mostly involves a single intervention mechanism,which is not conducive to the clinical transformation of this type of drugs.In the present study,rotenone was used to construct a PD cell model,and the protective effect of HSYA on the PD cell model was evaluated by cell viability and mitochondrial membrane potential.TTD database was used to query PD-related therapeutic targets,Swiss Target Prediction database to query HSYA-related targets,STRING database was used to search the gene interaction relationship of common targets,and ClueGO pathway was adopted to enrich and analyze common targets and their interaction targets,as well as to explore the comprehensive intervention mechanism.The results showed that rotenone could successfully establish the PD cell model,and HSYA had significant protective effect on PD cell model.Through the network pharmacological analysis,36 PD-related therapeutic targets and 88 HSYA-related targets were queried.The common targets of PD and HSYA were FKBP1A,HTR1A,SLC6A4 and SLC6A3.To enrich four common targets and their interaction targets through REACTOME pathway,eight cell signal pathways were obtained,and six cell biological processes were obtained through biological process pathway enrichment.展开更多
文摘Aim To study the proliferative effeet of hydroxysaftlor yellow A （HSYA） on cultured canine aortic endothelial cell （VEC） in normoxic （21% O2 ） or hypoxic （10% O2 ） culture and the underlying mechanism. Methods The endothelial cells were scratched from trypsined canine aorta endothelium. HSYA was added to the cells at final concentrations of 1 × 10^-3, 1 × 10^-4 and 1 × 10^-5 mol· L^-1, respectively. VEGF （2.6 × 10^-7 mol· L^-1 ）-treated cells were used as the positive control. The proliferative effect of HSYA on VEC was determined at 48, 72, 96, and 120 h in normoxic culture by MTI＂ assay. Similarly, the proliferation of VEC was determined at 12, 24, 48, and 72 h in hypoxic culture by MTF assay. The effects of HSYA on VEC proliferation and VEGF secretion were investigated by MTr and ELISA assays at the presence of the antibodies to VEGF and VEGF receptors. Results Pretreatment with HSYA at concentrations of 1 × 10^-3 and 1 × 10^-4 mol· L^-1 enhanced VEC proliferation in normoxic culture. The most significant enhancing effect of HSYA on VEC proliferation was achieved at 24, 48, and 72 h in hypoxic culture in concentration-dependent and time-dependent manner. HSYA at 1 × 10^-3 mol·L^-1 showed a potency similar to VEGF at 2.6 × 10^-7 mol·L^-1 . Pretreatment with the antibodies of Flt-1, KDR or VEGF blocked the proliferative effect of HSYA with similar potencies. Antibodies of Fit-1 or VEGF antagonized the promoting effect of HSYA on VEGF secretion. Conclusion HSYA promotes VEC proliferation either in normoxic or hypoxic culture, especially in the latter condition. This effect of HSYA is at least partly mediated by VEGF and VEGF receptor.
文摘目的:研究红花黄色素对神经根型腰椎病大鼠抗炎作用及其机制。方法:建立神经根型腰椎病大鼠模型,随机分为假手术组、模型组、PDTC(吡咯烷二硫代甲酸铵盐)组、红花黄色素100 mg/kg、50 mg/kg、25 mg/kg剂量组。红花黄色素各组于造模后3 d静脉注射给药,共给药7 d。检测后肢热痛刺激缩爪阈值的变化;椎板法检测血液流变学指标;Elisa法检测大鼠血清中TNF-α、IL-1β、IL-6、COX-2水平;Western法检测脊髓背角中p-NF-k B P65蛋白的表达;RT-PCR法检测NF-k B P65 mRNA的表达。结果:与模型组相比,100 mg/kg、50 mg/kg、25 mg/kg剂量的红花黄色素均能显著提高热痛刺激缩爪阈值、改善血液流变学各指标;显著降低TNF-α、IL-1β、IL-6、COX-2水平;显著抑制p-NF-k B P65蛋白及NF-k B P65 mRNA的表达。结论:红花黄色素可能对神经根型腰椎病的治疗有一定的意义,其机制与抑制NF-k B P65通路的激活有关。
基金Changjiang Scholar and Innovative Research Team in University (Grant No. 985-2-063-112)Youth Research Fellowship of Chinese Center for Disease Control and Prevention (Grant No. 2009A203)
文摘Safflower is a popular Chinese medicinal plant and Safflower injection is extensively used for the clinical treatment of cerebrovascular and cardiovascular diseases. In this study, HPLC-DAD-ESI-MSn was utilized to study the stability and degradation of the two major but chemically unstable bioactive compounds hydroxysaffior yellow A and anhydrosaffior yellow B, in Safflower injection. The impact of light irradiation, temperature, and pH on the stability of these two compounds were studied. The results showed that hydroxysafflor yellow A and anhydrosafflor yellow B could degrade at high temperature （〉60 ℃） or extreme pHs （pH ≤ 3.0 or 〉7.0）, but not under light irradiation. The common degradation product was p-coumaric acid. Chemical structures of the other degradation products were characterized by LC-MS. Hypothetical degradation pathways were proposed. In addition, ADP-induced platelet aggregation tests showed that the degradation of anhydrosaffior yellow B could reduce the anticoagulation activities of Safflower injection. Our results suggest that temperature and pH are critically important for the preparation and storage of Safflower injection.
文摘OBJECTIVE:To study the effects of hydroxy safflower yellow A(HSYA) on tumor capillary angiogenesis in transplanted human gastric adenocarcinoma BGC-823 tumors in nude mice.METHODS:BGC-823 cells were injected subcutaneously into the right anterior armpit of nude mice to establish an animal model of transplanted tumors.After 24 h,18 nude mice injected with tumor cells were randomized into model,control,and HSYA 0.028 g/L groups,with six mice in each group.Transplanted tumors were excised on day 20.Tumor inhibition ratios were calculated for the transplanted tumors.Pathological changes and capillary angiogenesis in the tumors were observed by light microscopy.RESULTS:Tumors in the model group grew more quickly than those in the control and HSYA groups,with inhibition ratios of 48% and 30%,respectively.The microvessel count in the HSYA group was lower than in the model group(P<0.01),and microvessel density was also lower in the HSYA group(P<0.05).Pathological changes were more obvious in tumors in the model group compared to the HSYA group.CONCLUSION:HSYA inhibits the growth of transplanted BGC-823 tumors,and its effects on tumor capillary angiogenesis may represent one of the mechanisms responsible for this antineoplastic effect.
基金Supported by Liaoning Province Science and Technology Plan Projects,Traditional Chinese Medicine Efficacy Evaluation Key Technology Research(No.2010225034)and(No.2010ZX09401-304)
文摘OBJECTIVE： To evaluate the clinical efficacy of safflower yellow injection combined with conventional therapy in treating unstable angina pectoris.METHODS： We searched online databases： Chinese journal full-text database, China National Knowledge Infrastructure, Wanfang database, Chinese journal full-text database, Pubmed, ScienceDirect,Embase, and the Cochrane Library with manual-screening of relevant literature. Eligible randomized controlled trials（RCT） on angina pectoris were included. We conducted meta-analysis using the RevMan 5.1 software from The Cochrane Collaboration. We treated the relief rate of angina symptoms and electrocardiograph（ECG） as evaluation.RESULTS： Seven articles, including in 1134 patients, were enrolled after the evaluation. Therewas no significant heterogeneity among the studies（χ2=1.08, df=6, P=0.98, I2=0%）. The safflower yellow injection with conventional therapy has a higher effective rate than the control group in relieving the symptoms of angina pectoris [odds ratio（OR）=2.95, 95%（CI）（1.81, 4.81）] and improving ischemic ECG [OR=2.85, 95% CI（1.67, 4.86）]. The difference was statistically significant in the ＂80 mg dosage＂ and ＂100 mg dosage＂ subgroups（P0.05） for improving clinical symptoms and ECG. The funnel graphic was nearly symmetrical. Sensitivity analysis suggested that the results were stable.CONCLUSION： Safflower yellow injection as an adjunct therapy with conventional drugs shows advantages in easing the clinical symptoms of unstable angina and improving ECG over basic therapy alone. However, the conclusions should be interpreted with care until more high-quality RCTs are reported.
基金Education Department of Hebei Province(Grant No.ZD2019107)。
文摘As an effective component of safflower,hydroxysafflor yellow A(HSYA)has the effect of promoting blood circulation,removing blood stasis,and relieving pain,and it has a certain effect on blood stasis and wind-induced Parkinson‘s disease(PD).However,the current research mostly involves a single intervention mechanism,which is not conducive to the clinical transformation of this type of drugs.In the present study,rotenone was used to construct a PD cell model,and the protective effect of HSYA on the PD cell model was evaluated by cell viability and mitochondrial membrane potential.TTD database was used to query PD-related therapeutic targets,Swiss Target Prediction database to query HSYA-related targets,STRING database was used to search the gene interaction relationship of common targets,and ClueGO pathway was adopted to enrich and analyze common targets and their interaction targets,as well as to explore the comprehensive intervention mechanism.The results showed that rotenone could successfully establish the PD cell model,and HSYA had significant protective effect on PD cell model.Through the network pharmacological analysis,36 PD-related therapeutic targets and 88 HSYA-related targets were queried.The common targets of PD and HSYA were FKBP1A,HTR1A,SLC6A4 and SLC6A3.To enrich four common targets and their interaction targets through REACTOME pathway,eight cell signal pathways were obtained,and six cell biological processes were obtained through biological process pathway enrichment.
