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黄粉虫纤溶酶基因生物信息学初步分析 被引量:2
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作者 陈建兴 萨如拉 +3 位作者 李静 康玉洁 马晓东 王彩霞 《赤峰学院学报(自然科学版)》 2016年第14期5-7,共3页
通过对黄粉虫(Tenebrio molitor)纤溶酶基因序列进行生物信息学分析,结果发现黄粉虫的纤溶酶基因共包含6个外显子和5个内含子.整个CDS区共翻译出258个氨基酸,这些氨基酸以丝氨酸的比例最高,其次为甘氨酸,最低的为谷氨酸.黄粉虫纤溶酶基... 通过对黄粉虫(Tenebrio molitor)纤溶酶基因序列进行生物信息学分析,结果发现黄粉虫的纤溶酶基因共包含6个外显子和5个内含子.整个CDS区共翻译出258个氨基酸,这些氨基酸以丝氨酸的比例最高,其次为甘氨酸,最低的为谷氨酸.黄粉虫纤溶酶基因的编码存在明显的密码子使用偏好,其偏倚程度从天冬酰胺(N)的0%到谷氨酸(E)的100%.预测黄粉虫纤溶酶肽链的等电点p I在8.34左右,相对分子质量为26KD.黄粉虫纤溶酶多肽链是以亲水区和疏水区相互交错出现构成的.另外,黄粉虫纤溶酶蛋白质的二级结构多为β折叠,其次为无规卷曲,螺旋结构所占比重较轻. 展开更多
关键词 黄粉虫 纤溶酶基因 生物信息学分析 密码子使用偏好
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RT-PCR克隆人纤溶酶cDNA大片段
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作者 王立强 冯立 +3 位作者 张志强 孙艳 王洪军 吴益民 《生物技术》 CAS CSCD 2006年第2期38-39,共2页
目的:以人胎儿肝脏总RNA为模板,克隆人纤溶酶(plasmin)cDNA大片段。方法:采用异硫氰酸胍一步法提取总RNA,RT-PCR技术获取目的基因。结果:成功地扩增出1.74kb的人纤溶酶基因,并研究了扩增人纤溶酶cDNA大片段的特定实验条件。结论:为克隆c... 目的:以人胎儿肝脏总RNA为模板,克隆人纤溶酶(plasmin)cDNA大片段。方法:采用异硫氰酸胍一步法提取总RNA,RT-PCR技术获取目的基因。结果:成功地扩增出1.74kb的人纤溶酶基因,并研究了扩增人纤溶酶cDNA大片段的特定实验条件。结论:为克隆cDNA大片段研究提供了简便、快速的方法。 展开更多
关键词 RT-PCR扩增 纤溶酶基因 总RNA
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土鳖虫纤溶酶编码区序列的克隆与表达
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作者 李兴暖 何巍 +2 位作者 周裔春 黄宇峰 韩雅莉 《中国中药杂志》 CAS CSCD 北大核心 2010年第15期1925-1930,共6页
目的:获得土鳖虫纤溶酶编码区序列,并进行原核和真核表达。方法:根据已报道的多种动物纤溶酶基因cDNA序列设计引物,用RT-PCR和3′RACE法克隆得到土鳖虫纤溶酶编码区序列;将该序列克隆进入大肠杆菌和毕赤酵母进行表达。结果:序列分析表明... 目的:获得土鳖虫纤溶酶编码区序列,并进行原核和真核表达。方法:根据已报道的多种动物纤溶酶基因cDNA序列设计引物,用RT-PCR和3′RACE法克隆得到土鳖虫纤溶酶编码区序列;将该序列克隆进入大肠杆菌和毕赤酵母进行表达。结果:序列分析表明,所克隆的纤溶酶编码区序列长672bp,共编码224个氨基酸残基,起始氨基酸序列为IVGG,与多种动物纤溶酶一致。将此cDNA序列在大肠杆菌和毕赤酵母中进行表达,前者获得没有活性的表达蛋白,后者获得具有纤溶活性的重组表达蛋白。结论:首次报道了土鳖虫纤溶酶编码区序列,并进行了初步表达,为进一步研究其功能奠定了基础。 展开更多
关键词 土鳖虫 纤溶酶基因 克隆 表达
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急性心肌梗死静脉溶栓治疗42例临床分析 被引量:1
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作者 李传波 田慧杰 《交通医学》 2008年第1期54-54,共1页
目的:研究急性心肌梗死院前及急诊静脉溶栓治疗的效果。方法:对42例急性心肌梗死患者给予基因重组型人组织纤溶酶原激活酶rt-PA静脉溶栓,观察再通率、死亡率、并发症。结果:总再通率76.2%,死亡2例(4.8%),无严重并发症。结论:急性心肌梗... 目的:研究急性心肌梗死院前及急诊静脉溶栓治疗的效果。方法:对42例急性心肌梗死患者给予基因重组型人组织纤溶酶原激活酶rt-PA静脉溶栓,观察再通率、死亡率、并发症。结果:总再通率76.2%,死亡2例(4.8%),无严重并发症。结论:急性心肌梗死静脉溶栓治疗具有重要临床意义。 展开更多
关键词 急性心肌梗死 基因重组型人组织原激活(rt—PA) 静脉
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外伤性黄斑裂孔的治疗及研究进展 被引量:1
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作者 王星 彭惠 《眼科新进展》 CAS 北大核心 2019年第6期583-588,共6页
外伤性黄斑裂孔(traumatic macular hole,TMH)主要是指眼球在受到直接或间接、闭合或开放的外力创伤下立即或延迟发生的黄斑中心凹区的视网膜裂孔,在组织学上是从内界膜到感光细胞层的部分或全部组织缺损。目前的主要非手术治疗包括:定... 外伤性黄斑裂孔(traumatic macular hole,TMH)主要是指眼球在受到直接或间接、闭合或开放的外力创伤下立即或延迟发生的黄斑中心凹区的视网膜裂孔,在组织学上是从内界膜到感光细胞层的部分或全部组织缺损。目前的主要非手术治疗包括:定期随访观察,口服血塞通、地塞米松等药物,手术治疗方式为玻璃体切除联合气体或硅油填充术,术中内界膜(inner limiting membrane,ILM)剥除等,术中辅助使用转化生长因子-β2、自体浓缩血小板、血清填充裂孔等。治疗进展主要包括局部非甾体类抗炎药物促进裂孔自愈,玻璃体切除手术系统进步、手术治疗方式增加(ILM移植术、ILM翻瓣术、自体晶状体囊膜移植术等)、术中辅助药物更新(基因重组纤溶酶等)。各种治疗干预方式中,松解黄斑裂孔周围牵拉、提供Muller细胞增生支架和促进光感受器功能恢复是需要关注研究的重点,更安全的生物黏合剂、生物填充材料、干细胞移植、组织工程视网膜移植等可能是未来值得期待的治疗手段。本文就TMH治疗及研究进展进行综述。 展开更多
关键词 外伤性黄斑裂孔 玻璃体视网膜手术 非手术治疗 研究进展 内界膜翻瓣术 晶状体囊膜移植术 基因重组
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A novel animal model for in vivo study of liver cancer metastasis 被引量:6
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作者 Shinsuke Fujiwara Hikaru Fujioka +7 位作者 Chise Tateno Ken Taniguchi Masahiro Ito Hiroshi Ohishi Rie Utoh Hiromi Ishibashi Takashi Kanematsu Katsutoshi Yoshizato 《World Journal of Gastroenterology》 SCIE CAS CSCD 2012年第29期3875-3882,共8页
AIM:To establish an animal model with human hepatocyte-repopulated liver for the study of liver cancer metastasis.METHODS:Cell transplantation into mouse livers was conducted using alpha-fetoprotein(AFP)-producing hu-... AIM:To establish an animal model with human hepatocyte-repopulated liver for the study of liver cancer metastasis.METHODS:Cell transplantation into mouse livers was conducted using alpha-fetoprotein(AFP)-producing hu-man gastric cancer cells(h-GCCs) and h-hepatocytes as donor cells in a transgenic mouse line expressing urokinase-type plasminogen activator(uPA) driven by the albumin enhancer/promoter crossed with a severe combined immunodeficient(SCID) mouse line(uPA/SCID mice).Host mice were divided into two groups(A and B).Group A mice were transplanted with h-GCCs alone,and group B mice were transplanted with h-GCCs and h-hepatocytes together.The replacement index(RI),which is the ratio of transplanted h-GCCs and h-hepatocytes that occupy the examined area of a histological section,was estimated by measuring h-AFP and h-albumin concentrations in sera,respectively,as well as by immunohistochemical analyses of h-AFP and human cytokeratin 18 in histological sections.RESULTS:The h-GCCs successfully engrafted,repopulated,and colonized the livers of mice in group A(RI = 22.0% ± 2.6%).These mice had moderately differentiated adenocarcinomatous lesions with disrupted glandular structures,which is a characteristics feature of gastric cancers.The serum h-AFP level reached 211.0 ± 142.2 g/mL(range,7.1-324.2 g/mL).In group B mice,the h-GCCs and h-hepatocytes independently engrafted,repopulated the host liver,and developed colonies(RI = 12.0% ± 6.8% and 66.0% ± 12.3%,respectively).h-GCC colonies also showed typical adenocarcinomatous glandular structures around the h-hepatocyte-colonies.These mice survived for the full 56 day-study and did not exhibit any metastasis of h-GCCs in the extrahepatic regions during the observational period.The mice with an h-hepatocyte-repopulated liver possessed metastasized h-GCCs and therefore could be a useful humanized liver animal model for studying liver cancer metastasis in vivo.CONCLUSION:A novel animal model of human liver cancer metastasis was established using the uPA/SCID mouse line.This model could be useful for in vivo testing of anti-cancer drugs and for studying the mechanisms of human liver cancer metastasis. 展开更多
关键词 Urokinase-type plasminogen activator/severe combined immunodeficient mouse Mouse with humanized liver Liver cancer metastasis Alpha-fetoprotein-producing gastric cancer cells
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Reactive protein, plasminogen activator inhibitor type-1 (PAI-1) levels, PAI-1 promoter 4G/5G polymorphism and acute myocardial infarction
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作者 Xue-Lei Cao Chang-Yong Zhou +4 位作者 Lei Yin Shao-Chun Wang Xiu-Ling Jia Huan Huang Xiao-Hong Sun 《Journal of Geriatric Cardiology》 SCIE CAS CSCD 2010年第3期147-151,共5页
Objective To investigate the relationship between CRP, plasminogen activator inhibitor type 1 (PAI-1) levels, PAI-1 gene promoter 4G/5G polymorphism and the type of acute myocardial infarction (ST elevation myocard... Objective To investigate the relationship between CRP, plasminogen activator inhibitor type 1 (PAI-1) levels, PAI-1 gene promoter 4G/5G polymorphism and the type of acute myocardial infarction (ST elevation myocardial infarction, STEMI vs the non-ST elevation Myocardial infarction, NSTEMI). Methods One hundred seventy-six consecutive patients with AMI were included for the study, of whom 60 had STEMI and 56 had NSTEMI, and 60 adults without cardiovascular and cerebrovascular disease were selected as controls. Blood samples were obtained from patients within 6 h of AMI and the plasma PAI-1, CRP, and the gene polymorphism were measured. Results Plasma levels of PAI- 1 and CRP were higher in AMI groups, compared those in the control group, and plasma levels of PAI-1 were significantly higher in patients with STEMI compared to those with NSTEMI (80.12ng/ml VS.73.01ng/ml, P 〈0.01), while CRP levels were not significantly different between patient with STEMI and NSTEMI (3.87 ± 0.79 mg/ml VS.4.01 ±0.69mg/ml, P〉0.05). PAI-1 levels presented a significant correlation with CRP levels in the NSTEMI subjects. However, PAI-1 and CRP levels could explain the lack of a significant relationship between them in control and STEMI subjects.The frequencies of 4G/4G genotype in the AMI group were higher than those in the control group and higher in patient with STEMI than in patient with NSTEMI. Plasma levels of PAI-1 in subjects with 4G/4G genotype were significantly increased as compared to those in subjects with 4G/5G and 5G/5G genotype. Conclusions Plasma PAI-1 levels were associated with different myocardial infarction type, and PAI-1 promoter 4G/5G polymorphisms and CRP may be related to plasma PAI-1 levels 展开更多
关键词 ST-segment elevation myocardial infarction non-ST segment elevation myocardial infarction Plasminogen activatorinhibitor- 1 C-reactive protein
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Relationship between gene polymorphism of the PAI-1 promoter and myocardial infarction 被引量:3
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作者 富路 金红 +3 位作者 宋克宁 张翠丽 沈景霞 黄永麟 《Chinese Medical Journal》 SCIE CAS CSCD 2001年第3期42-45,105-106,共6页
abstractObjective To investigate the association between gene polymorphism of the plasminogen activator inhibitor 1 (PAI 1) and myocardial infarction (MI) in Chinese Methods PAI 1 genotyping with polymerase chai... abstractObjective To investigate the association between gene polymorphism of the plasminogen activator inhibitor 1 (PAI 1) and myocardial infarction (MI) in Chinese Methods PAI 1 genotyping with polymerase chain reaction restriction fragment length polymorphism (PCR RFLP) and allele specific polymerase chain reaction (ASPCR) was performed in 87 myocardial infarction patients and 92 unrelated healthy controls All subjects'clinical features and PAI 1 activity were tested Results There were two polymorphisms within the promoter, a G/A single base substitution polymorphism upstream at -844?bp, and a single guanosine deletion/insertion 4G/5G polymorphism -675?bp upstream from the start of transcription Significant differences between the patients and the controls were observed neither for the frequencies of the GG, GA and AA genotypes nor for the PAI 1 activities of these three types But for the 4G/5G polymorphism, there were significant differences between patients and controls for the frequencies of the 4G/4G, 4G/5G and 5G/5G genotypes ( P <0 05) In the MI group, the PAI 1 activity of the 4G/4G type was significantly higher than that of the 5G/5G type ( P <0 05) Further more, a positive correlation between the glucose level and PAI 1 activity was found ( r =0 34, P =0 02) Conclusion This study indicates that the 4G/5G gene polymorphism of PAI 1 is associated with myocardial infarction, that 4G/4G type is probably an important hereditary risk factor, and that glucose has functional importance in regulating PAI 1 activity 展开更多
关键词 plasminogen activator inhibitor 1 · gene polymorphism · myocardial infarction
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