Pretreatment of the corn stover powder by dilute sulphuric acid （solid-liquid ratio 1 ： 20） at 130 for 30 min was carried out with 89.09% of the hemicellulose removed. After filtration, the xylose-rich corn stover ...Pretreatment of the corn stover powder by dilute sulphuric acid （solid-liquid ratio 1 ： 20） at 130 for 30 min was carried out with 89.09% of the hemicellulose removed. After filtration, the xylose-rich corn stover pretreatment liquid, whose fermentable sugar was from hemicellulose hydrolysis only, consisting of 81.16% xylose and 15.27% glucose, was used to cultivate genetic recombinant Escherichia coli BL21 with human-like collagen （HLC） expression enhanced by 50.00% and 63.71% xylose consumption.展开更多
The pretreatment of rice straw is often used to enhance the hydrolysis. 1-allyl-3-methylimidazolium chloride （ [ AMIM ] C1） is a kind of low viscous, nontoxic and recyclable ionic liquid. It was used to treat rice s...The pretreatment of rice straw is often used to enhance the hydrolysis. 1-allyl-3-methylimidazolium chloride （ [ AMIM ] C1） is a kind of low viscous, nontoxic and recyclable ionic liquid. It was used to treat rice straw and improve the enzymatic hydrolysis of rice straw in this study. The factors influencing the pretreatment were as follows： the dosage of rice straw in [ AMIM ] Cl, crush mesh of rice straw, pretreatment temperature and time. After the pretreatment with a 3 % （the weight ratio of rice straw to ionic liquid） rice straw dosage in [AMIM]Cl at 110 ℃ for 1 h, the yield of reducing sugar of regenerated rice straw by 33 U/mL cellulase hydrolysis was 53.3 %, which was two times higher than that of un-treated rice straw （23.7 % ）. More researches regarding straw biorefinery to bacterial cellulose are being performed in the lab and prospective results will be published in near future.展开更多
In this study we used l-allyl-3-methyl imidazole formate （[Amim][COOH]） as ionic liquid to pre-treat the cellulose and determined the rate of polymerization and enzymatic hydrolysis. The results showed that pretreat...In this study we used l-allyl-3-methyl imidazole formate （[Amim][COOH]） as ionic liquid to pre-treat the cellulose and determined the rate of polymerization and enzymatic hydrolysis. The results showed that pretreatment with （[Amim][COOH]） significantly decreased the cellulose polymerization. As the pretreatment temperature went up, the enzymatic hydrolysis rate was first increased and then decreased The maximal enzymatic hydrolysis rate was achieved when the pretreatment temperature was 90 ℃. Under the ultrasonic condition, the initial rate of enzmatic hydrolysis for the ionic liquid-treated cellulose was up to 11.10 gL-1h-1, which was 33% increase compared to the untreated cellulose. Scanning Electronic Microscopy （SEM） and Fourier Transform Infrared-Raman Spectroscop （FT-IR） analysis showed that ionic liquidtreated cellulose started to depolymerize. In addition, the cr3＇stallinity of the cellulose was significantly decreased after pretreatment with ionic liquid.展开更多
基金Supported by the Agriculture Application Investigation and Improvement Item of New Countryside Construction and Promotion Project of the Bureau of Science and Technology in Xi’an (NC08005)
文摘Pretreatment of the corn stover powder by dilute sulphuric acid （solid-liquid ratio 1 ： 20） at 130 for 30 min was carried out with 89.09% of the hemicellulose removed. After filtration, the xylose-rich corn stover pretreatment liquid, whose fermentable sugar was from hemicellulose hydrolysis only, consisting of 81.16% xylose and 15.27% glucose, was used to cultivate genetic recombinant Escherichia coli BL21 with human-like collagen （HLC） expression enhanced by 50.00% and 63.71% xylose consumption.
基金Science and Technology Commission of Shanghai Municipality (No.11230700600No.08520750200)+2 种基金Shanghai Municipal Education Commission (No.09ZZ68)the "111 " Project(No.B07024)the Fundamental Research Funds for the Central Universities and Key Laboratory of Science &Technology of Eco-Textile (Donghua University),Ministry of Education (No.Eco-op-2009-09)
文摘The pretreatment of rice straw is often used to enhance the hydrolysis. 1-allyl-3-methylimidazolium chloride （ [ AMIM ] C1） is a kind of low viscous, nontoxic and recyclable ionic liquid. It was used to treat rice straw and improve the enzymatic hydrolysis of rice straw in this study. The factors influencing the pretreatment were as follows： the dosage of rice straw in [ AMIM ] Cl, crush mesh of rice straw, pretreatment temperature and time. After the pretreatment with a 3 % （the weight ratio of rice straw to ionic liquid） rice straw dosage in [AMIM]Cl at 110 ℃ for 1 h, the yield of reducing sugar of regenerated rice straw by 33 U/mL cellulase hydrolysis was 53.3 %, which was two times higher than that of un-treated rice straw （23.7 % ）. More researches regarding straw biorefinery to bacterial cellulose are being performed in the lab and prospective results will be published in near future.
文摘In this study we used l-allyl-3-methyl imidazole formate （[Amim][COOH]） as ionic liquid to pre-treat the cellulose and determined the rate of polymerization and enzymatic hydrolysis. The results showed that pretreatment with （[Amim][COOH]） significantly decreased the cellulose polymerization. As the pretreatment temperature went up, the enzymatic hydrolysis rate was first increased and then decreased The maximal enzymatic hydrolysis rate was achieved when the pretreatment temperature was 90 ℃. Under the ultrasonic condition, the initial rate of enzmatic hydrolysis for the ionic liquid-treated cellulose was up to 11.10 gL-1h-1, which was 33% increase compared to the untreated cellulose. Scanning Electronic Microscopy （SEM） and Fourier Transform Infrared-Raman Spectroscop （FT-IR） analysis showed that ionic liquidtreated cellulose started to depolymerize. In addition, the cr3＇stallinity of the cellulose was significantly decreased after pretreatment with ionic liquid.
