[Objective]The aim was to isolate cellulose decomposing strains with high activity from straw-amended soil.[Method]Screening of CMC solid culture and shaking culture rescreening were carried out to obtain cellulose de...[Objective]The aim was to isolate cellulose decomposing strains with high activity from straw-amended soil.[Method]Screening of CMC solid culture and shaking culture rescreening were carried out to obtain cellulose decomposing strains with high activity from the corn straw amended soil,which were analyzed by 16rDNA sequence analysis.[Result]A bacterium and a fungus with higher endonucleases activity were obtained through preliminary screening and secondary screening,a fungus with higher filter paper enzyme activity,and a bacterium(NO.5 strain) with higher of both filter paper enzyme activity and CMC enzyme activity were obtained.The result of 16S rDNA sequence analysis showed that the similarity was 100% between NO.5 strain and Bacillus subtilis.[Conclusion]NO.5 strain was identified as Bacillus subtilis.展开更多
This study almed to screen a highIy efficient ceI uIose-degrading compIex microbial system from soiI and rotten straw and then study its appIication to natural ceI uIose. The isoIated stralns were preIiminariIy screen...This study almed to screen a highIy efficient ceI uIose-degrading compIex microbial system from soiI and rotten straw and then study its appIication to natural ceI uIose. The isoIated stralns were preIiminariIy screened with Congo red stalning and the ceI uIases activities were determined with DNS method. The non-antagonis-tic highIy efficient ceI uIos-degrading stralns were seIected and cuItured combinedIy for deveIoping a ceI uIose-degrading compIex microbial system. The resuIts showed that the CMC enzyme activity of the mixed cuIture of the three fungi stralns was higher than those of the cuItures of the three singIe stralns. The morphoIogical and moIecuIar bioIogical identification indicated that F1 was Botryosphaeria, F2 was Rhi-zopus oryzae, and F5 was Fusarium oxysporum. When straw was used as carbon source, the CMC enzyme activities of F1, F2 and F5 were 39.2, 31.4 and 40.6 IU/mI, respectiveIy; whiIe the CMC enzyme activity of the mixed cuIture of F1+F2+F5 was 50.12 IU/mI, which was increased by 23% compared to that of the singIe straln of F5. The experimental resuIts indicated that the ceI uIose-degrading effect of the compIex microbial system was better than those of the singIe fungi stralns, and the singIe stralns of F1, F2 and F5 had certaln deveIopment potentials.展开更多
[Objective] The research aimed to lay the foundation for high-efficiency biological degradation microbial inoculums. [Method] under the 60℃ temperature the cellulolytic microbes in pig manure were isolated and determ...[Objective] The research aimed to lay the foundation for high-efficiency biological degradation microbial inoculums. [Method] under the 60℃ temperature the cellulolytic microbes in pig manure were isolated and determined the CMCase and Fpase,then proceeded the 16S rRNA gene analysis. [Result]The results showed that:BC1 and BC3 strains characterized higher carboxymethyl cellulose enzyme activity and higher filter paper activity,but their difference was small,then the 16S rDNA sequence of BC1 and BC3 strains were related to pseudomonas sp. (98% and 99% similarities,respectively).[Conclusion] the experiment laid foundation for high-efficiency biological heating agent.展开更多
In this paper, in order to get the target microbe which has high cellulose bio-degradation ability, we collected soil samples from environment rich in cellulose. Carboxymethyl cellulose sodium gel plate and filter pap...In this paper, in order to get the target microbe which has high cellulose bio-degradation ability, we collected soil samples from environment rich in cellulose. Carboxymethyl cellulose sodium gel plate and filter paper plate were used for microbe screening, and finally we got a strain named MI which has the highest cellulase producing ability, and it has been identified as Penicilium oxalicum by morphological and molecular biological identification. We have studied cellulose degradation ability of this strain under different conditions such as pH value, temperature and inoculating time. The results showed that CMCase which belongs to acid enzyme, could achieve its maximum enzyme activity 489.96 U/ml when the optimal pH value was 4. The FPAase could achieve its maximum enzyme activity 1,595.45 U/ml when the optimal pH value was 4, too. The optimal ferment temperature of CMCase is 50℃ when achieved the maximum of enzyme activity at the second day, whereas the FPAase could reach its tip top on the third day, So the optimal ferment temperature of FPAase is also 50℃.展开更多
NaA zeolite membranes were prepared by secondary growth method on the outer surface of α-Al2O3 hollow fiber supports. Vacuum seeding method was used for planting zeolite seeds on the support surfaces. Hydrothermal cr...NaA zeolite membranes were prepared by secondary growth method on the outer surface of α-Al2O3 hollow fiber supports. Vacuum seeding method was used for planting zeolite seeds on the support surfaces. Hydrothermal crystallization was then carried out in a synthesis solution with molar ratio of Al2O3:SiO2:Na2O:H2O = 1:2:2:120 at 100 ℃ for 4 h. Effects of seeding conditions on preparation of hollow fiber NaA zeolite membranes were extensively investigated. Moreover, hollow fiber membrane modules with packing membrane areas of ca. 0.1 and 0.2 m2 were fabricated to separate ethanol/water mixture. It is found that the thickness of seed layer is obviously affected by seed suspension concentration, coating time and vacuum degree. Close-packing seed layer is required to obtain high-quality membranes. The optimized seeding conditions (seed suspension mass concentration of 0.5%-0.7% coating time of 5 s and vacuum degree of 10 kPa) lead to dense NaA zeolite layer with a thickness of 6-8 gin. Typically, an as-synthesized hollow fiber NaA zeolite membrane exhibits good pervaporation performance with a permeation flux of 7.02 kg· m^- 2· h^- 1 and separation factor 〉 10000 for sepa- ration of 90%; (by mass) ethanol/water mixture at 75 ℃ High reproducibility has been achieved for batch-scale production of hollow fiber NaA zeolite membranes by the hydrothermal synthesis approach.展开更多
This work presents the design of hollow fiber T-type zeolite membrane modules with different geometric configurations. The module performances were evaluated by pervaporation dehydration of ethanol/water mixtures. Str...This work presents the design of hollow fiber T-type zeolite membrane modules with different geometric configurations. The module performances were evaluated by pervaporation dehydration of ethanol/water mixtures. Strong concentration polarization was found for the modules with big membrane bundles. The concentration polarization was enhanced at high temperature due to the higher water permeation flux. The increase of feed flow could improve water permeation flux for the membrane modules with small membrane bundle.Computational fluid dynamics was used to visualize the flow field distribution inside of the modules with different configurations. The membrane module with seven bundles exhibited highest separation efficiency due to the uniform distribution of flow rate. The packing density could be 10 times higher than that of the tubular membrane module. The hollow fiber membrane module exhibited good stability for ethanol dehydration.展开更多
A non-invasive diagnostic approach is crucial for the evaluation of severity of liver disease,treatment decisions,and assessing drug efficacy.This study evaluated plasma proteomic profiling via an N-terminal isotope t...A non-invasive diagnostic approach is crucial for the evaluation of severity of liver disease,treatment decisions,and assessing drug efficacy.This study evaluated plasma proteomic profiling via an N-terminal isotope tagging strategy coupled with liquid chromatography/Fourier transform ion cyclotron resonance mass spectrometry measurement to detect liver fibrosis staging.Pooled plasma from different liver fibrosis stages,which were assessed in advance by the current gold-standard of liver biopsy,was quantitatively analyzed.A total of 72 plasma proteins were found to be dysregulated during the fibrogenesis process,and this finding constituted a valuable candidate plasma biomarker bank for follow-up analysis.Validation results of fibronectin by Western blotting reconfirmed the mass-based data.Ingenuity Pathways Analysis showed four types of metabolic networks for the functional effect of liver fibrosis disease in chronic hepatitis B patients.Consequently,quantitative proteomics via the N-terminal acetyl isotope labeling technique provides an effective and useful tool for screening plasma candidate biomarkers for liver fibrosis.We quantitatively monitored the fibrogenesis process in CHB patients.We discovered many new valuable candidate biomarkers for the diagnosis of liver fibrosis and also partly identified the mechanism involved in liver fibrosis disease.These results provide a clearer understanding of liver fibrosis pathophysiology and will also hopefully lead to improvement of clinical diagnosis and treatment.展开更多
基金Supported by Natural Foundation of Education Department of Henan Province (2010B530001) Science and Technology Innovation Supporting Program Talents of University in Henan Province(2010HASTIT019)+1 种基金Key Technology Research and Demonstration of Large-scale Straw Gasification Equipment (092102310044)Key Technology Research and Demonstration of Large Cascade Straw Digestion Biogas Generating Installation (2009A180013)~~
文摘[Objective]The aim was to isolate cellulose decomposing strains with high activity from straw-amended soil.[Method]Screening of CMC solid culture and shaking culture rescreening were carried out to obtain cellulose decomposing strains with high activity from the corn straw amended soil,which were analyzed by 16rDNA sequence analysis.[Result]A bacterium and a fungus with higher endonucleases activity were obtained through preliminary screening and secondary screening,a fungus with higher filter paper enzyme activity,and a bacterium(NO.5 strain) with higher of both filter paper enzyme activity and CMC enzyme activity were obtained.The result of 16S rDNA sequence analysis showed that the similarity was 100% between NO.5 strain and Bacillus subtilis.[Conclusion]NO.5 strain was identified as Bacillus subtilis.
基金Supported by Key Program for International S&T Cooperation Projects of China(2012DFA30600)National Key Technology Research and Development Program(2012BAD29B06)Special Scientific Research Fund of Marine Public Welfare Profession of China(201305013)~~
文摘This study almed to screen a highIy efficient ceI uIose-degrading compIex microbial system from soiI and rotten straw and then study its appIication to natural ceI uIose. The isoIated stralns were preIiminariIy screened with Congo red stalning and the ceI uIases activities were determined with DNS method. The non-antagonis-tic highIy efficient ceI uIos-degrading stralns were seIected and cuItured combinedIy for deveIoping a ceI uIose-degrading compIex microbial system. The resuIts showed that the CMC enzyme activity of the mixed cuIture of the three fungi stralns was higher than those of the cuItures of the three singIe stralns. The morphoIogical and moIecuIar bioIogical identification indicated that F1 was Botryosphaeria, F2 was Rhi-zopus oryzae, and F5 was Fusarium oxysporum. When straw was used as carbon source, the CMC enzyme activities of F1, F2 and F5 were 39.2, 31.4 and 40.6 IU/mI, respectiveIy; whiIe the CMC enzyme activity of the mixed cuIture of F1+F2+F5 was 50.12 IU/mI, which was increased by 23% compared to that of the singIe straln of F5. The experimental resuIts indicated that the ceI uIose-degrading effect of the compIex microbial system was better than those of the singIe fungi stralns, and the singIe stralns of F1, F2 and F5 had certaln deveIopment potentials.
基金Supported by Foundation of Henan Educational Committee(2010B530001)~~
文摘[Objective] The research aimed to lay the foundation for high-efficiency biological degradation microbial inoculums. [Method] under the 60℃ temperature the cellulolytic microbes in pig manure were isolated and determined the CMCase and Fpase,then proceeded the 16S rRNA gene analysis. [Result]The results showed that:BC1 and BC3 strains characterized higher carboxymethyl cellulose enzyme activity and higher filter paper activity,but their difference was small,then the 16S rDNA sequence of BC1 and BC3 strains were related to pseudomonas sp. (98% and 99% similarities,respectively).[Conclusion] the experiment laid foundation for high-efficiency biological heating agent.
文摘In this paper, in order to get the target microbe which has high cellulose bio-degradation ability, we collected soil samples from environment rich in cellulose. Carboxymethyl cellulose sodium gel plate and filter paper plate were used for microbe screening, and finally we got a strain named MI which has the highest cellulase producing ability, and it has been identified as Penicilium oxalicum by morphological and molecular biological identification. We have studied cellulose degradation ability of this strain under different conditions such as pH value, temperature and inoculating time. The results showed that CMCase which belongs to acid enzyme, could achieve its maximum enzyme activity 489.96 U/ml when the optimal pH value was 4. The FPAase could achieve its maximum enzyme activity 1,595.45 U/ml when the optimal pH value was 4, too. The optimal ferment temperature of CMCase is 50℃ when achieved the maximum of enzyme activity at the second day, whereas the FPAase could reach its tip top on the third day, So the optimal ferment temperature of FPAase is also 50℃.
基金Supported by the National Basic Research Program of China(2009CB623403)the National Natural Science Foundation of China(21176117,21222602)+3 种基金the Key Project of Chinese Ministry of Education(212060)the Outstanding Young Fund of Jiangsu Province(BK2012040)the"Six Top Talents"of Jiangsu Province,the Natural Science Foundation of the Jiangsu Higher Education Institutions of China(09KJA530002)a project funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD)
文摘NaA zeolite membranes were prepared by secondary growth method on the outer surface of α-Al2O3 hollow fiber supports. Vacuum seeding method was used for planting zeolite seeds on the support surfaces. Hydrothermal crystallization was then carried out in a synthesis solution with molar ratio of Al2O3:SiO2:Na2O:H2O = 1:2:2:120 at 100 ℃ for 4 h. Effects of seeding conditions on preparation of hollow fiber NaA zeolite membranes were extensively investigated. Moreover, hollow fiber membrane modules with packing membrane areas of ca. 0.1 and 0.2 m2 were fabricated to separate ethanol/water mixture. It is found that the thickness of seed layer is obviously affected by seed suspension concentration, coating time and vacuum degree. Close-packing seed layer is required to obtain high-quality membranes. The optimized seeding conditions (seed suspension mass concentration of 0.5%-0.7% coating time of 5 s and vacuum degree of 10 kPa) lead to dense NaA zeolite layer with a thickness of 6-8 gin. Typically, an as-synthesized hollow fiber NaA zeolite membrane exhibits good pervaporation performance with a permeation flux of 7.02 kg· m^- 2· h^- 1 and separation factor 〉 10000 for sepa- ration of 90%; (by mass) ethanol/water mixture at 75 ℃ High reproducibility has been achieved for batch-scale production of hollow fiber NaA zeolite membranes by the hydrothermal synthesis approach.
基金Supported by the National Natural Science Foundation of China(21222602,21490585 and 21176117)National High-tech R&D Program of China(2015AA03A602)+4 种基金the Key Project of Chinese Ministry of Education(212060)the Outstanding Young Fund of Jiangsu Province(BK2012040)Young Fund of Jiangsu Province(BK20130915)the "Six Top Talents" and "333 Talent Project" of Jiangsu Province,a project funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD)the Innovation Project for Graduate Student of Jiangsu Province(KYLX15-0790)
文摘This work presents the design of hollow fiber T-type zeolite membrane modules with different geometric configurations. The module performances were evaluated by pervaporation dehydration of ethanol/water mixtures. Strong concentration polarization was found for the modules with big membrane bundles. The concentration polarization was enhanced at high temperature due to the higher water permeation flux. The increase of feed flow could improve water permeation flux for the membrane modules with small membrane bundle.Computational fluid dynamics was used to visualize the flow field distribution inside of the modules with different configurations. The membrane module with seven bundles exhibited highest separation efficiency due to the uniform distribution of flow rate. The packing density could be 10 times higher than that of the tubular membrane module. The hollow fiber membrane module exhibited good stability for ethanol dehydration.
基金supported by the National Basic Research Program of China(Grant Nos.2011CB910601,2011CB910700,2010CB912700 and 2011CB505304)the National High Technology Research and Development Program of China (Grant No.2006AA02A308)+4 种基金National Natural Science Foundation of China(Grant Nos.30700356,30700988,30972909,81000192,81001470 and 81010064)Chinese State Key Project Specialized for Infectious Diseases (Grant Nos.2008ZX10002-016 and 2009ZX10004-103)the National Key Technologies R&D Program for New Drugs (Grant No.2009ZX09301-002)the International Scientific Collaboration Program (Grant Nos.2009DFB33070 and 2010DFA31260)State Key Laboratory of Proteomics(Grant Nos.SKLP-Y200901 and SKLP-O200901)
文摘A non-invasive diagnostic approach is crucial for the evaluation of severity of liver disease,treatment decisions,and assessing drug efficacy.This study evaluated plasma proteomic profiling via an N-terminal isotope tagging strategy coupled with liquid chromatography/Fourier transform ion cyclotron resonance mass spectrometry measurement to detect liver fibrosis staging.Pooled plasma from different liver fibrosis stages,which were assessed in advance by the current gold-standard of liver biopsy,was quantitatively analyzed.A total of 72 plasma proteins were found to be dysregulated during the fibrogenesis process,and this finding constituted a valuable candidate plasma biomarker bank for follow-up analysis.Validation results of fibronectin by Western blotting reconfirmed the mass-based data.Ingenuity Pathways Analysis showed four types of metabolic networks for the functional effect of liver fibrosis disease in chronic hepatitis B patients.Consequently,quantitative proteomics via the N-terminal acetyl isotope labeling technique provides an effective and useful tool for screening plasma candidate biomarkers for liver fibrosis.We quantitatively monitored the fibrogenesis process in CHB patients.We discovered many new valuable candidate biomarkers for the diagnosis of liver fibrosis and also partly identified the mechanism involved in liver fibrosis disease.These results provide a clearer understanding of liver fibrosis pathophysiology and will also hopefully lead to improvement of clinical diagnosis and treatment.