目的:研究神经生长因子对拟阿尔茨海默病(A lzhe im er's D isease,AD)模型大鼠海马及皮层中神经原纤维缠结(NFT)的影响。方法:采用冈田酸(Okadaic Ac id,OA)海马CA1区微量多次注射建立拟AD大鼠模型,侧脑室注射神经生长因子(nerve g...目的:研究神经生长因子对拟阿尔茨海默病(A lzhe im er's D isease,AD)模型大鼠海马及皮层中神经原纤维缠结(NFT)的影响。方法:采用冈田酸(Okadaic Ac id,OA)海马CA1区微量多次注射建立拟AD大鼠模型,侧脑室注射神经生长因子(nerve growth factor,NGF)进行预处理,观察大鼠行为学变化,B ielschowsky染色观察海马及皮层神经原纤维缠结(neurobrillary tangles,NFT)。结果:OA注射后,大鼠出现认知能力、学习记忆能力减退,海马及皮层出现较多NFT;注射NGF预处理后,上述症状改善,海马及皮层NFT数量减少。结论:NGF可显著改善拟AD大鼠模型学习记忆能力,表明NGF可抑制NFT的形成,改善AD病的症状。展开更多
Objective: Parkinsonian signs, especially gait impairment, are common and associated with morbidity and mortality in older persons. Our objective was to test the hypothesis that substantia nigra neurofibrillary tangle...Objective: Parkinsonian signs, especially gait impairment, are common and associated with morbidity and mortality in older persons. Our objective was to test the hypothesis that substantia nigra neurofibrillary tangles (NFTs) are related to parkinsonian signs in older persons with and without dementia. Methods: We studied 86 deceased older Catholic clergy without idiopathic Parkinson’s disease from the Religious Order Study, a longitudinal clinical-pathological study. Mean age at death was 85.3 years. Signs of gait disturbance, bradykinesia, rigidity, and tremor were assessed proximate to death using a modified Unified Parkinson’s Disease Rating Scale. Forty-micrometer paraffin-embedded sections of substantia nigra were bleached before tau immunohistochemistry and the optical disector was used to count NFTs. We used multivariable linear regression to examine parkinsonian signs as a function of nigra NFTs, controlling for age, sex, education, and cortical NFTs. Results: Substantia nigra NFTs were present in 67 of 86 persons (77.9% ). After controlling for age, sex, education, and cortical NFTs, nigra NFTs were related to gait impairment (p < 0.001), but not bradykinesia, rigidity, or tremor. Results were not confounded by dementia, Braak score, neuroleptic medication, cerebral infarcts, or Lewy bodies. Interpretation: NFTs in the substantia nigra are associated with gait impairment in older persons with and without dementia.展开更多
Brains of 41 residents without neurodegenerative diseases in the high-incid ence area of ALS/parkinsonism-dementia complex (ALS/PDC) of the Kii Peninsula of Japan were neuropathologically examined. Neurofibrillary tan...Brains of 41 residents without neurodegenerative diseases in the high-incid ence area of ALS/parkinsonism-dementia complex (ALS/PDC) of the Kii Peninsula of Japan were neuropathologically examined. Neurofibrillary tangles (NFTs) in th e hippocampal area were present in 11 of the 41 cases, but the frequency of NFT -positive cases in each age group was similar to that of the normal Japanese p opulation and far less than that of Guamanians without ALS/PDC.展开更多
文摘目的:研究神经生长因子对拟阿尔茨海默病(A lzhe im er's D isease,AD)模型大鼠海马及皮层中神经原纤维缠结(NFT)的影响。方法:采用冈田酸(Okadaic Ac id,OA)海马CA1区微量多次注射建立拟AD大鼠模型,侧脑室注射神经生长因子(nerve growth factor,NGF)进行预处理,观察大鼠行为学变化,B ielschowsky染色观察海马及皮层神经原纤维缠结(neurobrillary tangles,NFT)。结果:OA注射后,大鼠出现认知能力、学习记忆能力减退,海马及皮层出现较多NFT;注射NGF预处理后,上述症状改善,海马及皮层NFT数量减少。结论:NGF可显著改善拟AD大鼠模型学习记忆能力,表明NGF可抑制NFT的形成,改善AD病的症状。
文摘Objective: Parkinsonian signs, especially gait impairment, are common and associated with morbidity and mortality in older persons. Our objective was to test the hypothesis that substantia nigra neurofibrillary tangles (NFTs) are related to parkinsonian signs in older persons with and without dementia. Methods: We studied 86 deceased older Catholic clergy without idiopathic Parkinson’s disease from the Religious Order Study, a longitudinal clinical-pathological study. Mean age at death was 85.3 years. Signs of gait disturbance, bradykinesia, rigidity, and tremor were assessed proximate to death using a modified Unified Parkinson’s Disease Rating Scale. Forty-micrometer paraffin-embedded sections of substantia nigra were bleached before tau immunohistochemistry and the optical disector was used to count NFTs. We used multivariable linear regression to examine parkinsonian signs as a function of nigra NFTs, controlling for age, sex, education, and cortical NFTs. Results: Substantia nigra NFTs were present in 67 of 86 persons (77.9% ). After controlling for age, sex, education, and cortical NFTs, nigra NFTs were related to gait impairment (p < 0.001), but not bradykinesia, rigidity, or tremor. Results were not confounded by dementia, Braak score, neuroleptic medication, cerebral infarcts, or Lewy bodies. Interpretation: NFTs in the substantia nigra are associated with gait impairment in older persons with and without dementia.
文摘Brains of 41 residents without neurodegenerative diseases in the high-incid ence area of ALS/parkinsonism-dementia complex (ALS/PDC) of the Kii Peninsula of Japan were neuropathologically examined. Neurofibrillary tangles (NFTs) in th e hippocampal area were present in 11 of the 41 cases, but the frequency of NFT -positive cases in each age group was similar to that of the normal Japanese p opulation and far less than that of Guamanians without ALS/PDC.
