[Objective] The research aimed to breed the high-yield production strain of cellulase.[Method] Aspergillus niger was used as the starting strain,and a high-yield production strain of cellulase was selected after UV mu...[Objective] The research aimed to breed the high-yield production strain of cellulase.[Method] Aspergillus niger was used as the starting strain,and a high-yield production strain of cellulase was selected after UV mutation treatment.[Result] Under the suitable condition,the strain 2(15) with the highest CMC production capacity was selected,which nearly increased 50% than that of the starting strain.[Conclusion] The research provided the foundation for its appliation in the feed production in the future.展开更多
This study was conducted to investigate the effect of feeding different fibre feedstuffs on the performance, nutrient utilization and economics of production of weaning/growing pigs. Five fibre feedstuffs--palm kernel...This study was conducted to investigate the effect of feeding different fibre feedstuffs on the performance, nutrient utilization and economics of production of weaning/growing pigs. Five fibre feedstuffs--palm kernel cake (PKC), wheat offal (WO), corn bran (CB), rice bran (RB) and brewers' dried grain (BDG) were used at 25% in each of the five experimental diets that were formulated in this study. Twenty weaner pigs (12.75 ± 0.6 kg) were used for the growth study while 15 pigs (14.95 ± 0.57 kg) were used for the digestibility study. The design of the experiment was completely randomized. The results of the study showed that the performance of the experimental animals were significantly influenced (P 〈 0.05) by the dietary treatments. The apparent digestibility of the crude protein, crude fiber and ether extract showed significant differences (P 〈 0.05) while there was no significant difference (P 〉 0.05) in the apparent digestibility of their dry matter, ash and nitrogen free extract. The diet that contained WO had the best growth response and apparent nutrient digestibility. Results of economics of production also showed that diet with WO gave the best (P 〈 0.05) value in feed cost/'kg gain and net profit/animal. It can be concluded from this study that although all the animals fed the different fibre feedstuffs performed satisfactorily on all the parameters monitored, but for optimum growth, apparent nutrients digestibility and economics of production. WO should be used as a fibre feedstuff in the diets of weaning/growing pigs in the tropics.展开更多
The concentration of volatile fatty acids (VFA) determined from a previous study was used to determine the concentration of carbon dioxide (CO2) and methane (CH4) in pig digesta using the model developed for rum...The concentration of volatile fatty acids (VFA) determined from a previous study was used to determine the concentration of carbon dioxide (CO2) and methane (CH4) in pig digesta using the model developed for ruminant animals. Digesta from the stomach, cecum and colon of pigs (n = 3 dietl) were used for the determination of VFA. The pigs were fed either a low fiber diet (LFD; 8.3% aNDF) as control, or one of the high-fiber diets (HFD, 22.4% aNDF; D1, D2 and D3) containing 1:2, 1:1 and 2:1, oats to barley ratios, respectively. Results indicated that the concentrations of CO2 and CH4 in pigs fed HFD were, on the average, 25.8 and 24.2%, respectively lower (P 〈 0.05) than pigs fed LFD. Pigs fed the highest oat to barley ratio also showed higher (P 〈 0.05) levels of CO2, (8.3%) and CH4 (5.1%), compared to the average of the two lower ratios (CO2, 5.3% and CH4, 3.3%). Molar proportions of VFA, CO2 and CH4 in the gut were in the order of VFA 〉 CO2 〉 CH4, at 53.0%, 28.6% and 18.4%, respectively, and CO2 and CH4 combined represented 47% of total gas.展开更多
The investigation was carried out with altogether 332 growing rabbits (ZIKA) in six rounds of 8 weeks duration respectively (R1-R6) with the following occupational objects: wooden gnawing stick (WGS), plastic g...The investigation was carried out with altogether 332 growing rabbits (ZIKA) in six rounds of 8 weeks duration respectively (R1-R6) with the following occupational objects: wooden gnawing stick (WGS), plastic gnawing stick (PGS) and two iron chains connected by a horizontal stick (ICS), wooden gnawing limb (WGL) and a horizontal iron chain (HIC). The feeding system varied from pellet feeding ad libitum (in the first rounds, in the following rounds it decreased pro-rata whereas the proportion of the crude fibre feed (loose or pressed in blocks) increased appropriately) to crude fibre feeding ad libitum. The behavior "occupation with the tool" was infrared video recorded and continuously analyzed and summarized in hour-values per rabbit. The average frequency of occupation per rabbit and hour ranged from 0,13 to 2.19 concerning the rounds and between 0.12 and 1,30 concerning the tools. Altogether, it could be assumed that different occupational tools had an influence on the engaging behavior but the effect of the round which was affected by the feeding system was higher. The investigation showed that rabbits spent less time occupying with the tool when they were fed with an increasing percentage of crude fibre (less pellets) in the ration.展开更多
Three yearling lambs with a rumen cannula were used to investigate the effects of supplementation with an urea-minerals lick block (ULB) on the kinetics of ruminal fibre digestion, nutrient digestibility and nitrog...Three yearling lambs with a rumen cannula were used to investigate the effects of supplementation with an urea-minerals lick block (ULB) on the kinetics of ruminal fibre digestion, nutrient digestibility and nitrogen (N) utilization office straw (RS), ammonia bicarbonate (AB)-treated RS (ABRS) and hay prepared from natural pasture. The digestibility of dry matter and organic matter of RS increased by 13.1% and 12.7% (P〈0.05) when the diet was supplemented with ULB, and approached to that of ABRS, indicating that the effect of ULB on digestibility of RS is similar to that of AB treatment. The digestibility of ABRS was slightly improved by the ULB feeding. Nitrogen retention was highest in lambs fed on ABRS alone, followed by hay with ULB, and was lowest in animals fed on RS with ULB. However, both the amount and proportion of N retention to N intake were enhanced by ULB supplementation to lambs fed on hay. The proportion of N retained to N digested decreased due to ULB supplementation to lambs fed on RS or ABRS. Supplementing ULB did not greatly influence the rumen degradation of either dry matter or crude protein in each of the three diets. RS and hay had similar values in the potential extent of digestion (PED) and digestion rate of PED (kd) of fibrous materials, but the discrete lag time for RS was lower than that for hay. The AB treatment significantly increased the PED (P〈0.05) and kd (P〈0.05) of RS. Neither the PED nor kd for RS and ABRS was influenced by ULB supplementation, but the kd for hay significantly increased due to ULB. The lag time for hay was also shortened by the ULB feeding. The ULB improved the digestion of fibre in the rumen of lambs fed on low quality roughage. It is inferred that while ULB is effective in increasing nutrient digestibility of low quality roughages by improving ruminal fibre digestion. A synchronized supply of N and energy to rumen microbes should be considered to improve the efficiency of N utilization when the basal diet is ammoniated straw.展开更多
This experiment was conducted using by-products from agro-industry as dairy cows feed. Bagasse, pineapple peel, corn cob, corn stover and vinasses were used to produce TMF (total mixed fiber) for dairy cow feed. Fif...This experiment was conducted using by-products from agro-industry as dairy cows feed. Bagasse, pineapple peel, corn cob, corn stover and vinasses were used to produce TMF (total mixed fiber) for dairy cow feed. Fifteen multiparous Holstein Friesian dairy cows with an initial body weight of 417.88± 52.60 kg and 83.31 ± 26.47 DIM (days in milk) were randomly allocated to three treatments (TI = rice straw and 1 kg of vinasses; T2 = SCWS (sweet corn waste silage); T3 = TMF (total mixed fiber)) under completely randomized design. The results showed that the chemical composition of TMF was in the normal range of pH and VFA (volatile fatty acids) on silage. However, cows fed TMF and SCWS tended to yield higher level of NH3-N. For volatile fatty acid in rumen fluid, acetate in group of cows fed rice straw with vinasse tended to be the highest. Moreover, propionate in cows fed TMF was found to be the highest among all treatments (P 〉 0.05). In conclusion, the TMF can be used to feed dairy cow without affecting rumen parameter.展开更多
Ninety six pigs average weight 9.15 kg were used to study the effects of feeding diets containing multiple sources of dietary fiber (DF) on weight gain and concentration of fermentation products in the digestive tra...Ninety six pigs average weight 9.15 kg were used to study the effects of feeding diets containing multiple sources of dietary fiber (DF) on weight gain and concentration of fermentation products in the digestive tract. The experiment employed four diets and four pens diet1. Diets included a low fiber diet (LFD), as control and three antibiotic free high-fiber diets (HFD) containing a 1:2, 1:1 or 2:1 oats:barley ratios. At the end of the 70 d feeding trial three pigs of comparable weight diefI were slaughtered. Digesta taken from the stomach, cecum and colon were used for the determination of volatile fatty acids (VFA). Results indicated that inclusion of HFD increased (P 〈 0.05) feed intake but not weight gain. As revealed by the higher feed intake the nutritional value of HFD was approximately 88% of LFD. Also, as the amount of oats in the diet was increased, VFA concentration was also increased while isobutyrate decreased (P 〈 0.05). Lower concentration of isobutyrate may indicate parallel reduction in other putatively toxic products of protein fermentation in the digestive tract. Thus, inclusion of multiple sources of fiber in the diet impacted feed intake and the products of gut fermentation in growing pigs.展开更多
The study was conducted to investigate the effects of feeding high-fiber diet (HFD) on apparent total tract digestibility (ATTD) and absorption of minerals in growing pigs. Sixteen barrows averaging 16.39 kg were ...The study was conducted to investigate the effects of feeding high-fiber diet (HFD) on apparent total tract digestibility (ATTD) and absorption of minerals in growing pigs. Sixteen barrows averaging 16.39 kg were randomly assigned to one of four diets after metabolic crate assignment with individual pig serving as experimental unit. Diets include: a corn-soybean meal based low-fiber diet (LFD), as the control (CON) and 3 HFD (D1, D2 and D3), each containing 5% sugar beet pulps and a 1:2, 1:1 and 2:1 oats to barley ratios, respectively, replacing 47% of the corn in the diet. Diets were formulated to meet nutrient requirements, however, the fiber content of the LFD was substantially lower than the average of the HFD (8.38% vs. 22.40%). Feed allowance was limited to 10% of average body weight and fed twice/d. The study period was divided into 10 d of adjustment and 5 d of feces collection. Data collected were analyzed using SAS, GLIMMIX procedure where animals were considered random effects, and minerals were fixed. Results of the study show that digestibility of Ca in pigs fed HFD was lower (P 〈 0.05) than in pigs fed LFD. On the other hand, the apparent digestibility of P, K, Na, S and Zn in pigs fed D2 and D3 were higher (P 〈 0.05) than in pigs fed CON diet. It was also observed that digestibility of Ca, P, K, Na, S and Zn in pigs fed D1 were lower (P 〈 0.05) than in CON, D2 or D3 fed pigs. Therefore, it was concluded that while feeding HFD reduced availability of Ca, the availability of P, K, Na and S to pigs improved as the oat to barley ratio in the diet increased.展开更多
基金Supported by Natural Foundation of Education Department of HenanProvince (2010B530001)Research Project of Department of Sci-ence and Technology in Henan Province (102102310391)~~
文摘[Objective] The research aimed to breed the high-yield production strain of cellulase.[Method] Aspergillus niger was used as the starting strain,and a high-yield production strain of cellulase was selected after UV mutation treatment.[Result] Under the suitable condition,the strain 2(15) with the highest CMC production capacity was selected,which nearly increased 50% than that of the starting strain.[Conclusion] The research provided the foundation for its appliation in the feed production in the future.
文摘This study was conducted to investigate the effect of feeding different fibre feedstuffs on the performance, nutrient utilization and economics of production of weaning/growing pigs. Five fibre feedstuffs--palm kernel cake (PKC), wheat offal (WO), corn bran (CB), rice bran (RB) and brewers' dried grain (BDG) were used at 25% in each of the five experimental diets that were formulated in this study. Twenty weaner pigs (12.75 ± 0.6 kg) were used for the growth study while 15 pigs (14.95 ± 0.57 kg) were used for the digestibility study. The design of the experiment was completely randomized. The results of the study showed that the performance of the experimental animals were significantly influenced (P 〈 0.05) by the dietary treatments. The apparent digestibility of the crude protein, crude fiber and ether extract showed significant differences (P 〈 0.05) while there was no significant difference (P 〉 0.05) in the apparent digestibility of their dry matter, ash and nitrogen free extract. The diet that contained WO had the best growth response and apparent nutrient digestibility. Results of economics of production also showed that diet with WO gave the best (P 〈 0.05) value in feed cost/'kg gain and net profit/animal. It can be concluded from this study that although all the animals fed the different fibre feedstuffs performed satisfactorily on all the parameters monitored, but for optimum growth, apparent nutrients digestibility and economics of production. WO should be used as a fibre feedstuff in the diets of weaning/growing pigs in the tropics.
文摘The concentration of volatile fatty acids (VFA) determined from a previous study was used to determine the concentration of carbon dioxide (CO2) and methane (CH4) in pig digesta using the model developed for ruminant animals. Digesta from the stomach, cecum and colon of pigs (n = 3 dietl) were used for the determination of VFA. The pigs were fed either a low fiber diet (LFD; 8.3% aNDF) as control, or one of the high-fiber diets (HFD, 22.4% aNDF; D1, D2 and D3) containing 1:2, 1:1 and 2:1, oats to barley ratios, respectively. Results indicated that the concentrations of CO2 and CH4 in pigs fed HFD were, on the average, 25.8 and 24.2%, respectively lower (P 〈 0.05) than pigs fed LFD. Pigs fed the highest oat to barley ratio also showed higher (P 〈 0.05) levels of CO2, (8.3%) and CH4 (5.1%), compared to the average of the two lower ratios (CO2, 5.3% and CH4, 3.3%). Molar proportions of VFA, CO2 and CH4 in the gut were in the order of VFA 〉 CO2 〉 CH4, at 53.0%, 28.6% and 18.4%, respectively, and CO2 and CH4 combined represented 47% of total gas.
文摘The investigation was carried out with altogether 332 growing rabbits (ZIKA) in six rounds of 8 weeks duration respectively (R1-R6) with the following occupational objects: wooden gnawing stick (WGS), plastic gnawing stick (PGS) and two iron chains connected by a horizontal stick (ICS), wooden gnawing limb (WGL) and a horizontal iron chain (HIC). The feeding system varied from pellet feeding ad libitum (in the first rounds, in the following rounds it decreased pro-rata whereas the proportion of the crude fibre feed (loose or pressed in blocks) increased appropriately) to crude fibre feeding ad libitum. The behavior "occupation with the tool" was infrared video recorded and continuously analyzed and summarized in hour-values per rabbit. The average frequency of occupation per rabbit and hour ranged from 0,13 to 2.19 concerning the rounds and between 0.12 and 1,30 concerning the tools. Altogether, it could be assumed that different occupational tools had an influence on the engaging behavior but the effect of the round which was affected by the feeding system was higher. The investigation showed that rabbits spent less time occupying with the tool when they were fed with an increasing percentage of crude fibre (less pellets) in the ration.
基金Project (No. 011102193) supported by the Foundation of the Science and Technology Commission of Zhejiang Provincethe Foundation for Excellent Youth Teachers from the State Commission of Education, China
文摘Three yearling lambs with a rumen cannula were used to investigate the effects of supplementation with an urea-minerals lick block (ULB) on the kinetics of ruminal fibre digestion, nutrient digestibility and nitrogen (N) utilization office straw (RS), ammonia bicarbonate (AB)-treated RS (ABRS) and hay prepared from natural pasture. The digestibility of dry matter and organic matter of RS increased by 13.1% and 12.7% (P〈0.05) when the diet was supplemented with ULB, and approached to that of ABRS, indicating that the effect of ULB on digestibility of RS is similar to that of AB treatment. The digestibility of ABRS was slightly improved by the ULB feeding. Nitrogen retention was highest in lambs fed on ABRS alone, followed by hay with ULB, and was lowest in animals fed on RS with ULB. However, both the amount and proportion of N retention to N intake were enhanced by ULB supplementation to lambs fed on hay. The proportion of N retained to N digested decreased due to ULB supplementation to lambs fed on RS or ABRS. Supplementing ULB did not greatly influence the rumen degradation of either dry matter or crude protein in each of the three diets. RS and hay had similar values in the potential extent of digestion (PED) and digestion rate of PED (kd) of fibrous materials, but the discrete lag time for RS was lower than that for hay. The AB treatment significantly increased the PED (P〈0.05) and kd (P〈0.05) of RS. Neither the PED nor kd for RS and ABRS was influenced by ULB supplementation, but the kd for hay significantly increased due to ULB. The lag time for hay was also shortened by the ULB feeding. The ULB improved the digestion of fibre in the rumen of lambs fed on low quality roughage. It is inferred that while ULB is effective in increasing nutrient digestibility of low quality roughages by improving ruminal fibre digestion. A synchronized supply of N and energy to rumen microbes should be considered to improve the efficiency of N utilization when the basal diet is ammoniated straw.
文摘This experiment was conducted using by-products from agro-industry as dairy cows feed. Bagasse, pineapple peel, corn cob, corn stover and vinasses were used to produce TMF (total mixed fiber) for dairy cow feed. Fifteen multiparous Holstein Friesian dairy cows with an initial body weight of 417.88± 52.60 kg and 83.31 ± 26.47 DIM (days in milk) were randomly allocated to three treatments (TI = rice straw and 1 kg of vinasses; T2 = SCWS (sweet corn waste silage); T3 = TMF (total mixed fiber)) under completely randomized design. The results showed that the chemical composition of TMF was in the normal range of pH and VFA (volatile fatty acids) on silage. However, cows fed TMF and SCWS tended to yield higher level of NH3-N. For volatile fatty acid in rumen fluid, acetate in group of cows fed rice straw with vinasse tended to be the highest. Moreover, propionate in cows fed TMF was found to be the highest among all treatments (P 〉 0.05). In conclusion, the TMF can be used to feed dairy cow without affecting rumen parameter.
文摘Ninety six pigs average weight 9.15 kg were used to study the effects of feeding diets containing multiple sources of dietary fiber (DF) on weight gain and concentration of fermentation products in the digestive tract. The experiment employed four diets and four pens diet1. Diets included a low fiber diet (LFD), as control and three antibiotic free high-fiber diets (HFD) containing a 1:2, 1:1 or 2:1 oats:barley ratios. At the end of the 70 d feeding trial three pigs of comparable weight diefI were slaughtered. Digesta taken from the stomach, cecum and colon were used for the determination of volatile fatty acids (VFA). Results indicated that inclusion of HFD increased (P 〈 0.05) feed intake but not weight gain. As revealed by the higher feed intake the nutritional value of HFD was approximately 88% of LFD. Also, as the amount of oats in the diet was increased, VFA concentration was also increased while isobutyrate decreased (P 〈 0.05). Lower concentration of isobutyrate may indicate parallel reduction in other putatively toxic products of protein fermentation in the digestive tract. Thus, inclusion of multiple sources of fiber in the diet impacted feed intake and the products of gut fermentation in growing pigs.
文摘The study was conducted to investigate the effects of feeding high-fiber diet (HFD) on apparent total tract digestibility (ATTD) and absorption of minerals in growing pigs. Sixteen barrows averaging 16.39 kg were randomly assigned to one of four diets after metabolic crate assignment with individual pig serving as experimental unit. Diets include: a corn-soybean meal based low-fiber diet (LFD), as the control (CON) and 3 HFD (D1, D2 and D3), each containing 5% sugar beet pulps and a 1:2, 1:1 and 2:1 oats to barley ratios, respectively, replacing 47% of the corn in the diet. Diets were formulated to meet nutrient requirements, however, the fiber content of the LFD was substantially lower than the average of the HFD (8.38% vs. 22.40%). Feed allowance was limited to 10% of average body weight and fed twice/d. The study period was divided into 10 d of adjustment and 5 d of feces collection. Data collected were analyzed using SAS, GLIMMIX procedure where animals were considered random effects, and minerals were fixed. Results of the study show that digestibility of Ca in pigs fed HFD was lower (P 〈 0.05) than in pigs fed LFD. On the other hand, the apparent digestibility of P, K, Na, S and Zn in pigs fed D2 and D3 were higher (P 〈 0.05) than in pigs fed CON diet. It was also observed that digestibility of Ca, P, K, Na, S and Zn in pigs fed D1 were lower (P 〈 0.05) than in CON, D2 or D3 fed pigs. Therefore, it was concluded that while feeding HFD reduced availability of Ca, the availability of P, K, Na and S to pigs improved as the oat to barley ratio in the diet increased.