用表面修饰有羧基的生物磁粒对小麦种胚提取液中DNA 的纯化做了系统的研究,得到提取纯化的最佳条件.结果表明,在提取温度50～55℃、提取时间1 h、磁粒用量20 μL条件下,处理15 m g 样品可得到纯度很高的DNA 约43 μg.此法可直接在离心...用表面修饰有羧基的生物磁粒对小麦种胚提取液中DNA 的纯化做了系统的研究,得到提取纯化的最佳条件.结果表明,在提取温度50～55℃、提取时间1 h、磁粒用量20 μL条件下,处理15 m g 样品可得到纯度很高的DNA 约43 μg.此法可直接在离心管中操作,具有不离心,不接触氯仿、酚等有机萃取剂等特点.展开更多
Aim To screen the optimum macroporous resin and conditions for the isolation and purification of flavonoids from Radix Puerariae. Methods The static and dynamic adsorption/desorption methods were used, and the separat...Aim To screen the optimum macroporous resin and conditions for the isolation and purification of flavonoids from Radix Puerariae. Methods The static and dynamic adsorption/desorption methods were used, and the separation and purification process was evaluated by measuring the concentration of total flavonoid in the fractions with UV spectrophotometer. Results The SP70 macroporous resin was the most effective compared with other macroporous resins. The optimum conditions were screened, which were 0.5 g· mL^- 1 corresponding to crude drug for concentration of extract, pH 5 - 6, and appended 60 times the volume of the resin bed （BV） with the adsorption speed 2 BV·h^-1, and the volume of aq. 70% （V/V） ethanol as eluant was 5 BV with desorption speed 2 BV·h^-1. By this method, the final contents of total flavonoids exceeded 80%. Conclusion The SP70 macroporous resin is the most effective one for large-scale isolation and purification of flavonoids from Radix Pueraria, which meets industrial needs.展开更多
文摘用表面修饰有羧基的生物磁粒对小麦种胚提取液中DNA 的纯化做了系统的研究,得到提取纯化的最佳条件.结果表明,在提取温度50～55℃、提取时间1 h、磁粒用量20 μL条件下,处理15 m g 样品可得到纯度很高的DNA 约43 μg.此法可直接在离心管中操作,具有不离心,不接触氯仿、酚等有机萃取剂等特点.
基金Science and Technology Committee of Chongqing inChina(CSTC.2004BB5122).
文摘Aim To screen the optimum macroporous resin and conditions for the isolation and purification of flavonoids from Radix Puerariae. Methods The static and dynamic adsorption/desorption methods were used, and the separation and purification process was evaluated by measuring the concentration of total flavonoid in the fractions with UV spectrophotometer. Results The SP70 macroporous resin was the most effective compared with other macroporous resins. The optimum conditions were screened, which were 0.5 g· mL^- 1 corresponding to crude drug for concentration of extract, pH 5 - 6, and appended 60 times the volume of the resin bed （BV） with the adsorption speed 2 BV·h^-1, and the volume of aq. 70% （V/V） ethanol as eluant was 5 BV with desorption speed 2 BV·h^-1. By this method, the final contents of total flavonoids exceeded 80%. Conclusion The SP70 macroporous resin is the most effective one for large-scale isolation and purification of flavonoids from Radix Pueraria, which meets industrial needs.
