The dunite bodies, which extend as the direction of W-E, are exposed to the southeast of Elazlg located within the Eastern Taurus Belt of Turkey. Mafic-Ultramafic section in the Guleman ophiolite consists ofdunite whi...The dunite bodies, which extend as the direction of W-E, are exposed to the southeast of Elazlg located within the Eastern Taurus Belt of Turkey. Mafic-Ultramafic section in the Guleman ophiolite consists ofdunite which containing disseminated chromites, wehrlite, gabbros （isotrope gabbro and layered gabbro） and clinopyroxenite. Dunite blocks above the harzburgite massif have irregular contacts with the enclosing peridotites. Dunite blocks are generally around a few of meters. Dunite blocks consist of gabbro and pyroxenite patches. The origin of dunite blocks are belong to the transition zone of harzburgitic ophiolites which is located at the base of the mafic layered section. They are entirely or largely magmatic formed by olivine and chromite ponds at the base of the crustal magma chamber. The rather around of rock pieces within dunite bodies are foliated such as features have been ascribed to the ophiolite being impregnated by and reacting with a melt. Rocks in the bodies show depleted in incompatible trace elements such as Ba, Nbet al., characteristic of subduction related magma. Furthermore, the high LREE/HREE and high Rb/Th ratios indicates a mantle that has been enrichmented by subduction. As a result, isotopic data, petrographic and geochemical of bodies＇s result suggest a parental magma derived from an enrichmed source of subduction zone. A few meters of the large dunite bodies, and ascribes to the central dunites a cumulative origin by fractionation from a picritic melt.展开更多
As an excellent reporter molecule, enhanced green fluorescent protein (eGFP) was widely used for gene expression and regulation and was generally expressed in Escherichia coli strain. A rapid procedure consisting of a...As an excellent reporter molecule, enhanced green fluorescent protein (eGFP) was widely used for gene expression and regulation and was generally expressed in Escherichia coli strain. A rapid procedure consisting of ammonium sulfate precipitation, size exclusion chromatography, and anion exchange chromatography was devel- oped for the purification of eGFP. Based on the proposed procedure, recombinant eGFP with an electrophoretic pu- rity was achieved in combination with an overall yield of 66% and a purification factor of 17.9. The fluorescent spectrometry of purified eGFP and lysate from E. coli strain expressing eGFP exhibited the same wavelength of ex- citation and emission maxima, indicating that the purification procedure did not influence the construct and fluo- rescent characteristics of desired protein. The procedure mentioned was easy to scale up for the purification of large quantities of eGFP.展开更多
文摘The dunite bodies, which extend as the direction of W-E, are exposed to the southeast of Elazlg located within the Eastern Taurus Belt of Turkey. Mafic-Ultramafic section in the Guleman ophiolite consists ofdunite which containing disseminated chromites, wehrlite, gabbros （isotrope gabbro and layered gabbro） and clinopyroxenite. Dunite blocks above the harzburgite massif have irregular contacts with the enclosing peridotites. Dunite blocks are generally around a few of meters. Dunite blocks consist of gabbro and pyroxenite patches. The origin of dunite blocks are belong to the transition zone of harzburgitic ophiolites which is located at the base of the mafic layered section. They are entirely or largely magmatic formed by olivine and chromite ponds at the base of the crustal magma chamber. The rather around of rock pieces within dunite bodies are foliated such as features have been ascribed to the ophiolite being impregnated by and reacting with a melt. Rocks in the bodies show depleted in incompatible trace elements such as Ba, Nbet al., characteristic of subduction related magma. Furthermore, the high LREE/HREE and high Rb/Th ratios indicates a mantle that has been enrichmented by subduction. As a result, isotopic data, petrographic and geochemical of bodies＇s result suggest a parental magma derived from an enrichmed source of subduction zone. A few meters of the large dunite bodies, and ascribes to the central dunites a cumulative origin by fractionation from a picritic melt.
基金Supported by the National Natural Science Foundation of China (No. 20306024, No. 20476082), Tianjin Applied FundamentalResearch Project (No. 0436048-11) and the Program for Changjiang Scholars and Innovative Research Team in University from the Ministry of Education of China.
文摘As an excellent reporter molecule, enhanced green fluorescent protein (eGFP) was widely used for gene expression and regulation and was generally expressed in Escherichia coli strain. A rapid procedure consisting of ammonium sulfate precipitation, size exclusion chromatography, and anion exchange chromatography was devel- oped for the purification of eGFP. Based on the proposed procedure, recombinant eGFP with an electrophoretic pu- rity was achieved in combination with an overall yield of 66% and a purification factor of 17.9. The fluorescent spectrometry of purified eGFP and lysate from E. coli strain expressing eGFP exhibited the same wavelength of ex- citation and emission maxima, indicating that the purification procedure did not influence the construct and fluo- rescent characteristics of desired protein. The procedure mentioned was easy to scale up for the purification of large quantities of eGFP.
