目的 TiO2载银纳米羟基磷灰石/聚酰胺66(titania and Ag containing nano-hydroxyapatite/polyamide66,TiO2-Ag-nHA/PA66)复合骨填充材料具有良好的生物相容性及安全性,通过观察含不同Ag+浓度的TiO2-Ag-nHA/PA66复合骨填充材料的体外抗...目的 TiO2载银纳米羟基磷灰石/聚酰胺66(titania and Ag containing nano-hydroxyapatite/polyamide66,TiO2-Ag-nHA/PA66)复合骨填充材料具有良好的生物相容性及安全性,通过观察含不同Ag+浓度的TiO2-Ag-nHA/PA66复合骨填充材料的体外抗菌性能及Ag+释放特性,探讨其用于填充慢性骨髓炎术后骨缺损的可行性。方法采用常压共溶法合成n-HA/PA66复合骨填充材料A1(材料A1),并在其基础上制备Ag+含量分别为0.22wt%、0.64wt%,TiO2含量均为2.35wt%的TiO2-Ag-nHA/PA66复合骨填充材料A2、A3(材料A2、A3)。将材料A2、A3分别浸泡于50mL模拟体液(simulated body fluid,SBF)中,于1、3、7、14、21、49d采用原子吸收光谱法检测Ag+浓度。采用抑菌圈实验、菌落数实验检测材料A2、A3对金黄色葡萄球菌及大肠杆菌的体外抗菌效果,采用扫描电镜观察其对金黄色葡萄球菌及大肠杆菌的抗黏附效果;并以材料A1作对照。结果在SBF中浸泡1、3d材料A2、A3的Ag+浓度比较,差异无统计学意义(P>0.05);7d后差异均有统计学意义(P<0.05)。抑菌圈实验显示材料A2、A3第1天时对2种细菌的抑菌圈直径均最大,其中材料A2对金黄色葡萄球菌和大肠杆菌的抑菌圈直径分别为(13.40±2.88)mm及(9.40±1.14)mm,材料A3分别为(23.60±1.14)mm及(18.80±0.84)mm;同种细菌间2种材料比较,差异有统计学意义(P<0.05);之后随时间增加抑菌圈直径缩小,材料A2、A3产生的抑菌圈在金黄色葡萄球菌和大肠杆菌中持续时间分别为15、33d和9、24d;材料A1无抑菌圈产生。菌落数实验显示金黄色葡萄球菌、大肠杆菌与材料A1接触后继续生长繁殖,抗菌率为0;与材料A2、A3接触后生长显著减缓,抗菌率分别为89.74%±3.62%、94.18%±2.05%及78.65%±5.64%、85.96%±2.50%,同种细菌组间比较差异均有统计学意义(P<0.05)。扫描电镜观察材料A1表面有大量细菌黏附,呈团块状或线状分布;材料A2、A3表面黏附细菌明显较少,呈散在分布。结论 TiO2-Ag-nHA/PA66复合骨填充材料在体外对金黄色葡萄球菌及大肠杆菌有明显抗菌作用,在SBF中具有良好的缓释效果。展开更多
Nanocomposites of zinc/bentonite clay were synthesized for use as an antibacterial material by a quick and simple alkaline ion exchange method. The synthesis of zinc doped bentonite nanocomposite was accomplished by p...Nanocomposites of zinc/bentonite clay were synthesized for use as an antibacterial material by a quick and simple alkaline ion exchange method. The synthesis of zinc doped bentonite nanocomposite was accomplished by placing bentonite in a melting bath of ZnSO4 for 10, 20, 40, 60 and 90 rain. The complexes were characterized by XRD, SEM and DRS. XRD analyses and SEM observations confirmed the diffusion of zinc to the clay surfaces. Antibacterial activity tests against Escherichia coli showed that bentonite did not present any antibacterial properties, but after alkaline ion exchange treatment, inhibition was noted. The highest antibacterial activity was observed with ZnO/bentonite composite alkaline ion exchange for 60 and 90 rain. Interestingly, the leaching test indicated that ZnO/bentonite did not present any risk for drinking water treatment.展开更多
文摘目的 TiO2载银纳米羟基磷灰石/聚酰胺66(titania and Ag containing nano-hydroxyapatite/polyamide66,TiO2-Ag-nHA/PA66)复合骨填充材料具有良好的生物相容性及安全性,通过观察含不同Ag+浓度的TiO2-Ag-nHA/PA66复合骨填充材料的体外抗菌性能及Ag+释放特性,探讨其用于填充慢性骨髓炎术后骨缺损的可行性。方法采用常压共溶法合成n-HA/PA66复合骨填充材料A1(材料A1),并在其基础上制备Ag+含量分别为0.22wt%、0.64wt%,TiO2含量均为2.35wt%的TiO2-Ag-nHA/PA66复合骨填充材料A2、A3(材料A2、A3)。将材料A2、A3分别浸泡于50mL模拟体液(simulated body fluid,SBF)中,于1、3、7、14、21、49d采用原子吸收光谱法检测Ag+浓度。采用抑菌圈实验、菌落数实验检测材料A2、A3对金黄色葡萄球菌及大肠杆菌的体外抗菌效果,采用扫描电镜观察其对金黄色葡萄球菌及大肠杆菌的抗黏附效果;并以材料A1作对照。结果在SBF中浸泡1、3d材料A2、A3的Ag+浓度比较,差异无统计学意义(P>0.05);7d后差异均有统计学意义(P<0.05)。抑菌圈实验显示材料A2、A3第1天时对2种细菌的抑菌圈直径均最大,其中材料A2对金黄色葡萄球菌和大肠杆菌的抑菌圈直径分别为(13.40±2.88)mm及(9.40±1.14)mm,材料A3分别为(23.60±1.14)mm及(18.80±0.84)mm;同种细菌间2种材料比较,差异有统计学意义(P<0.05);之后随时间增加抑菌圈直径缩小,材料A2、A3产生的抑菌圈在金黄色葡萄球菌和大肠杆菌中持续时间分别为15、33d和9、24d;材料A1无抑菌圈产生。菌落数实验显示金黄色葡萄球菌、大肠杆菌与材料A1接触后继续生长繁殖,抗菌率为0;与材料A2、A3接触后生长显著减缓,抗菌率分别为89.74%±3.62%、94.18%±2.05%及78.65%±5.64%、85.96%±2.50%,同种细菌组间比较差异均有统计学意义(P<0.05)。扫描电镜观察材料A1表面有大量细菌黏附,呈团块状或线状分布;材料A2、A3表面黏附细菌明显较少,呈散在分布。结论 TiO2-Ag-nHA/PA66复合骨填充材料在体外对金黄色葡萄球菌及大肠杆菌有明显抗菌作用,在SBF中具有良好的缓释效果。
文摘Nanocomposites of zinc/bentonite clay were synthesized for use as an antibacterial material by a quick and simple alkaline ion exchange method. The synthesis of zinc doped bentonite nanocomposite was accomplished by placing bentonite in a melting bath of ZnSO4 for 10, 20, 40, 60 and 90 rain. The complexes were characterized by XRD, SEM and DRS. XRD analyses and SEM observations confirmed the diffusion of zinc to the clay surfaces. Antibacterial activity tests against Escherichia coli showed that bentonite did not present any antibacterial properties, but after alkaline ion exchange treatment, inhibition was noted. The highest antibacterial activity was observed with ZnO/bentonite composite alkaline ion exchange for 60 and 90 rain. Interestingly, the leaching test indicated that ZnO/bentonite did not present any risk for drinking water treatment.
