旨在预防猫、犬诱导的过敏反应,本研究将猫主要变应原Fel d 1与犬主要变应原Can f 1融合原核表达,纯化制备了rFel d 1-Can f 1融合变应原。将层状双氢氧化物(layered double hydroxides,LDH)与rFel d 1-Can f 1混合,制备LDH-rFel d 1-Ca...旨在预防猫、犬诱导的过敏反应,本研究将猫主要变应原Fel d 1与犬主要变应原Can f 1融合原核表达,纯化制备了rFel d 1-Can f 1融合变应原。将层状双氢氧化物(layered double hydroxides,LDH)与rFel d 1-Can f 1混合,制备LDH-rFel d 1-Can f 1,皮下注射法免疫BALB/c小鼠,随后利用rFel d 1-Can f 1融合变应原诱导过敏小鼠模型,通过检测小鼠应激性过敏反应的体温变化、耳朵点刺试验、肺部组织切片HE染色、血清IgE水平等指标评价LDH-rFel d 1-Can f 1对猫犬诱发过敏反应的预防效果。研究表明,LDH与rFel d 1-Can f 1质量比为7∶1时,LDH可以完全吸附rFel d 1-Can f 1融合蛋白。LDH-rFel d 1-Can f 1可以有效缓解应激性过敏反应引起的小鼠体温下降,显著减少过敏导致的染料渗透面积。肺部组织切片HE染色结果显示,LDH-rFel d 1-Can f 1可以缓解过敏引起的炎症细胞浸润。此外,LDH-rFel d 1-Can f 1免疫后可以诱导小鼠产生较高水平IgG,这为开发抑制宠物猫犬诱发过敏反应的疫苗奠定基础。展开更多
A poly(methyl acrylate)(PMA)/ZnAl layered double hydroxide(ZnAl-LDH) intercalation nanocomposite is synthesized by in situ polymerization of methyl acrylate with ogano-modified ZnAl-LDH(O-ZnAl-LDH). Its structure and ...A poly(methyl acrylate)(PMA)/ZnAl layered double hydroxide(ZnAl-LDH) intercalation nanocomposite is synthesized by in situ polymerization of methyl acrylate with ogano-modified ZnAl-LDH(O-ZnAl-LDH). Its structure and morphology are confirmed by X-ray diffraction(XRD) and transmission electron microscopy(TEM) image. The d 001 of O-ZnAl-LDH is expanded to 2.85 nm after polymerization from 2.63 nm, which indicates the intercalation of PMA chains into the galleries of O-ZnAl-LDH. The (001) diffraction of PMA/ZnAl-LDH nanocomposite is broad due to the exfoliation of some O-ZnAl-LDH layers. The TEM image shows that the most of the layers of O-ZnAl-LDH are stacked with a distance of about 3 nm while some of them are exfoliated and dispersed disorderly in the PMA matrix.展开更多
文摘旨在预防猫、犬诱导的过敏反应,本研究将猫主要变应原Fel d 1与犬主要变应原Can f 1融合原核表达,纯化制备了rFel d 1-Can f 1融合变应原。将层状双氢氧化物(layered double hydroxides,LDH)与rFel d 1-Can f 1混合,制备LDH-rFel d 1-Can f 1,皮下注射法免疫BALB/c小鼠,随后利用rFel d 1-Can f 1融合变应原诱导过敏小鼠模型,通过检测小鼠应激性过敏反应的体温变化、耳朵点刺试验、肺部组织切片HE染色、血清IgE水平等指标评价LDH-rFel d 1-Can f 1对猫犬诱发过敏反应的预防效果。研究表明,LDH与rFel d 1-Can f 1质量比为7∶1时,LDH可以完全吸附rFel d 1-Can f 1融合蛋白。LDH-rFel d 1-Can f 1可以有效缓解应激性过敏反应引起的小鼠体温下降,显著减少过敏导致的染料渗透面积。肺部组织切片HE染色结果显示,LDH-rFel d 1-Can f 1可以缓解过敏引起的炎症细胞浸润。此外,LDH-rFel d 1-Can f 1免疫后可以诱导小鼠产生较高水平IgG,这为开发抑制宠物猫犬诱发过敏反应的疫苗奠定基础。
文摘A poly(methyl acrylate)(PMA)/ZnAl layered double hydroxide(ZnAl-LDH) intercalation nanocomposite is synthesized by in situ polymerization of methyl acrylate with ogano-modified ZnAl-LDH(O-ZnAl-LDH). Its structure and morphology are confirmed by X-ray diffraction(XRD) and transmission electron microscopy(TEM) image. The d 001 of O-ZnAl-LDH is expanded to 2.85 nm after polymerization from 2.63 nm, which indicates the intercalation of PMA chains into the galleries of O-ZnAl-LDH. The (001) diffraction of PMA/ZnAl-LDH nanocomposite is broad due to the exfoliation of some O-ZnAl-LDH layers. The TEM image shows that the most of the layers of O-ZnAl-LDH are stacked with a distance of about 3 nm while some of them are exfoliated and dispersed disorderly in the PMA matrix.
