[Objective] This study was conducted to investigate the application of nanometallic materials in inhibiting Agrobacteriurn contamination in genetic transformation of Anthurium. [Method] Different nanometallic material...[Objective] This study was conducted to investigate the application of nanometallic materials in inhibiting Agrobacteriurn contamination in genetic transformation of Anthurium. [Method] Different nanometallic materials were added into Agrobacterium medium and Anthurium callus medium, to investigate the effects of their effects on Agrobacterium growth, callus growth and differentiation, and Agrobacterium contamination. [Result] Among the 4 nanometallic materials, NanoAg-2 showed a significant inhibitory effect on the growth of Agrobacterium, with a minimal inhibitory concentration of 25 mg/L. Even for the Anthurium calli or transgenic material contaminated by the Agrobacterium, a good antibacterial effect could be achieved after treating with 25 mg/L NanoAg-2 for 1 d with oscillation, the antibacterial rate reached 100%, and the Anthurium calli could grow and differentiate normally. [Conclusioa] NanoAg-2 could effectively inhibit Agrobacterium contamination, and its an- tibacterial effect is significantly better than cephalosporin and carbenicillin.展开更多
Antibacterial activity of boron-doped TiO2(B/TiO2) nano-materials under visible light irradiation and in the dark was investigated. A simple sol-gel method was used to synthesize TiO2 nano-materials. X-ray diffraction...Antibacterial activity of boron-doped TiO2(B/TiO2) nano-materials under visible light irradiation and in the dark was investigated. A simple sol-gel method was used to synthesize TiO2 nano-materials. X-ray diffraction pattern of B/TiO2 nano-materials represents the diffraction peaks relating to the crystal planes of TiO2(anatase and rutile). X-ray photoelectron spectroscopy result shows that part of boron ions incorporates into TiO2 lattice to form a possible chemical environment like Ti O B and the rest exist in the form of B2O3. The study on antibacterial effect of B/TiO2 nano-materials on fungal Candida albicans(ATCC10231), Gram-negative Escherichia coli(ATCC25922) and Gram-positive Staphylococcus aureus(ATCC6538) shows that the antibacterial action is more significant on Candida albicans than on Escherichia coli and Staphylococcus aureus. Under visible light irradiation, the antibacterial activity is superior to that in the dark.展开更多
Nanocomposites of zinc/bentonite clay were synthesized for use as an antibacterial material by a quick and simple alkaline ion exchange method. The synthesis of zinc doped bentonite nanocomposite was accomplished by p...Nanocomposites of zinc/bentonite clay were synthesized for use as an antibacterial material by a quick and simple alkaline ion exchange method. The synthesis of zinc doped bentonite nanocomposite was accomplished by placing bentonite in a melting bath of ZnSO4 for 10, 20, 40, 60 and 90 rain. The complexes were characterized by XRD, SEM and DRS. XRD analyses and SEM observations confirmed the diffusion of zinc to the clay surfaces. Antibacterial activity tests against Escherichia coli showed that bentonite did not present any antibacterial properties, but after alkaline ion exchange treatment, inhibition was noted. The highest antibacterial activity was observed with ZnO/bentonite composite alkaline ion exchange for 60 and 90 rain. Interestingly, the leaching test indicated that ZnO/bentonite did not present any risk for drinking water treatment.展开更多
In this paper, nanotechnology for the improvement of dental composite resins has been reviewed in the background of the existing shortcomings, focusing on the improvement for polymerization shrinkage, anti-bacterial p...In this paper, nanotechnology for the improvement of dental composite resins has been reviewed in the background of the existing shortcomings, focusing on the improvement for polymerization shrinkage, anti-bacterial properties and mechanical properties of composite resins. The results show that the use of nanotechnology and nano materials can be an effective method to improve the performance of dental composite resins in a various ways. At last, the paper also discusses the perspective about the dental composite resins.展开更多
Antibacterial nanoagents with broad-spectrum antibacterial activities have emerged as an evolution of antibiotics.However,their bacteria-capturing capability and bacteria-killing efficiency remain insufficient for com...Antibacterial nanoagents with broad-spectrum antibacterial activities have emerged as an evolution of antibiotics.However,their bacteria-capturing capability and bacteria-killing efficiency remain insufficient for commercial implementation.Inspired by the hunting behavior of spiders,here,a novel spiderweb-like nanoagent based on silver-adenine nanowires is designed to achieve high-efficiency capture and killing of bacteria.By virtue of the initiative bacteriacapturing functionality,this spiderweb-like nanoagent could effectively attract and trap bacteria through electrostatic interaction and its reticular morphology feature.Furthermore,its synergetic antibacterial mechanism combining physical membranolysis with reactive oxygen species(ROS)release allows such nanoagent to harvest a promoted bactericidal activity.Importantly,the woundplast employing the spiderweblike nanoagent exhibits a superior antibacterial efficacy against drug-resistant Gram-negative Escherichia coli and Gram-positive methicillin-resistant Staphylococcus aureus compared with commercially available woundplasts.This work may pave a new way for rational design of new generation bactericidal agents facing the future super-bacteria booming.展开更多
文摘[Objective] This study was conducted to investigate the application of nanometallic materials in inhibiting Agrobacteriurn contamination in genetic transformation of Anthurium. [Method] Different nanometallic materials were added into Agrobacterium medium and Anthurium callus medium, to investigate the effects of their effects on Agrobacterium growth, callus growth and differentiation, and Agrobacterium contamination. [Result] Among the 4 nanometallic materials, NanoAg-2 showed a significant inhibitory effect on the growth of Agrobacterium, with a minimal inhibitory concentration of 25 mg/L. Even for the Anthurium calli or transgenic material contaminated by the Agrobacterium, a good antibacterial effect could be achieved after treating with 25 mg/L NanoAg-2 for 1 d with oscillation, the antibacterial rate reached 100%, and the Anthurium calli could grow and differentiate normally. [Conclusioa] NanoAg-2 could effectively inhibit Agrobacterium contamination, and its an- tibacterial effect is significantly better than cephalosporin and carbenicillin.
基金Supported by the National Natural Science Foundation of China(51090384)
文摘Antibacterial activity of boron-doped TiO2(B/TiO2) nano-materials under visible light irradiation and in the dark was investigated. A simple sol-gel method was used to synthesize TiO2 nano-materials. X-ray diffraction pattern of B/TiO2 nano-materials represents the diffraction peaks relating to the crystal planes of TiO2(anatase and rutile). X-ray photoelectron spectroscopy result shows that part of boron ions incorporates into TiO2 lattice to form a possible chemical environment like Ti O B and the rest exist in the form of B2O3. The study on antibacterial effect of B/TiO2 nano-materials on fungal Candida albicans(ATCC10231), Gram-negative Escherichia coli(ATCC25922) and Gram-positive Staphylococcus aureus(ATCC6538) shows that the antibacterial action is more significant on Candida albicans than on Escherichia coli and Staphylococcus aureus. Under visible light irradiation, the antibacterial activity is superior to that in the dark.
文摘Nanocomposites of zinc/bentonite clay were synthesized for use as an antibacterial material by a quick and simple alkaline ion exchange method. The synthesis of zinc doped bentonite nanocomposite was accomplished by placing bentonite in a melting bath of ZnSO4 for 10, 20, 40, 60 and 90 rain. The complexes were characterized by XRD, SEM and DRS. XRD analyses and SEM observations confirmed the diffusion of zinc to the clay surfaces. Antibacterial activity tests against Escherichia coli showed that bentonite did not present any antibacterial properties, but after alkaline ion exchange treatment, inhibition was noted. The highest antibacterial activity was observed with ZnO/bentonite composite alkaline ion exchange for 60 and 90 rain. Interestingly, the leaching test indicated that ZnO/bentonite did not present any risk for drinking water treatment.
文摘In this paper, nanotechnology for the improvement of dental composite resins has been reviewed in the background of the existing shortcomings, focusing on the improvement for polymerization shrinkage, anti-bacterial properties and mechanical properties of composite resins. The results show that the use of nanotechnology and nano materials can be an effective method to improve the performance of dental composite resins in a various ways. At last, the paper also discusses the perspective about the dental composite resins.
基金supported by the National Natural Science Foundation of China(21703230,21635007 and 21721003)K.C.Wong Education Foundation。
文摘Antibacterial nanoagents with broad-spectrum antibacterial activities have emerged as an evolution of antibiotics.However,their bacteria-capturing capability and bacteria-killing efficiency remain insufficient for commercial implementation.Inspired by the hunting behavior of spiders,here,a novel spiderweb-like nanoagent based on silver-adenine nanowires is designed to achieve high-efficiency capture and killing of bacteria.By virtue of the initiative bacteriacapturing functionality,this spiderweb-like nanoagent could effectively attract and trap bacteria through electrostatic interaction and its reticular morphology feature.Furthermore,its synergetic antibacterial mechanism combining physical membranolysis with reactive oxygen species(ROS)release allows such nanoagent to harvest a promoted bactericidal activity.Importantly,the woundplast employing the spiderweblike nanoagent exhibits a superior antibacterial efficacy against drug-resistant Gram-negative Escherichia coli and Gram-positive methicillin-resistant Staphylococcus aureus compared with commercially available woundplasts.This work may pave a new way for rational design of new generation bactericidal agents facing the future super-bacteria booming.
