Theranostic nanoprobes can potentially integrate imaging and therapeutic capabilities into a single platform,offering a new personalized cancer diagnostic tool.However,there is a growing concern that their clinical ap...Theranostic nanoprobes can potentially integrate imaging and therapeutic capabilities into a single platform,offering a new personalized cancer diagnostic tool.However,there is a growing concern that their clinical application is not safe,particularly due to metal-containing elements,such as the gadolinium used in magnetic resonance imaging(MRI).We demonstrate for the first time that the photothermal melting of the DNA duplex helix was a reliable and versatile strategy that enables the on-demand degradation of the gadolinium-containing MRI reporter gene from polydopamine(PDA)-based theranostic nanoprobes.The combination of chemotherapy(doxorubicin)and photothermal therapy,which leads to the enhanced anti-tumor effect.In vivo MRI tracking reveals that renal filtration was able to rapidly clear the free gadolinium-containing MRI reporter from the mice body.This results in a decrease in the long-term toxic effect of theranostic MRI nanoprobes.Our findings may pave the way to address toxicity issues of the theranostic nanoprobes.展开更多
Detection of circulating tumor cells （CTCs） plays an important role in cancer diagnosis and prognosis. In this study, aptamer-conjugated upconversion nano- particles （UCNPs） are used for the first time as nanoprob...Detection of circulating tumor cells （CTCs） plays an important role in cancer diagnosis and prognosis. In this study, aptamer-conjugated upconversion nano- particles （UCNPs） are used for the first time as nanoprobes to recognize tumor cells, which are then enriched by attaching with magnetic nanoparticles （MNPs） and placing in the presence of a magnetic field. Owing to the autofluorescence- free nature of upconversion luminescence imaging, as well as the use of magnetic separation to further reduce background signals, our technique allows for highly sensitive detection and collection of small numbers of tumor cells spiked into healthy blood samples, and shows promise for CTC detection in medical diagnostics.展开更多
基金supported by the National Natural Science Foundation of China(21635007 and 21605137)the National Key Research and Development Program of China(2016YFA0203200)+2 种基金Natural Science Foundation of Shandong Province(2018GGX102030)Taishan Scholar Program of Shandong Province(ts201511027)K.C.Wong Education Foundation。
文摘Theranostic nanoprobes can potentially integrate imaging and therapeutic capabilities into a single platform,offering a new personalized cancer diagnostic tool.However,there is a growing concern that their clinical application is not safe,particularly due to metal-containing elements,such as the gadolinium used in magnetic resonance imaging(MRI).We demonstrate for the first time that the photothermal melting of the DNA duplex helix was a reliable and versatile strategy that enables the on-demand degradation of the gadolinium-containing MRI reporter gene from polydopamine(PDA)-based theranostic nanoprobes.The combination of chemotherapy(doxorubicin)and photothermal therapy,which leads to the enhanced anti-tumor effect.In vivo MRI tracking reveals that renal filtration was able to rapidly clear the free gadolinium-containing MRI reporter from the mice body.This results in a decrease in the long-term toxic effect of theranostic MRI nanoprobes.Our findings may pave the way to address toxicity issues of the theranostic nanoprobes.
文摘Detection of circulating tumor cells （CTCs） plays an important role in cancer diagnosis and prognosis. In this study, aptamer-conjugated upconversion nano- particles （UCNPs） are used for the first time as nanoprobes to recognize tumor cells, which are then enriched by attaching with magnetic nanoparticles （MNPs） and placing in the presence of a magnetic field. Owing to the autofluorescence- free nature of upconversion luminescence imaging, as well as the use of magnetic separation to further reduce background signals, our technique allows for highly sensitive detection and collection of small numbers of tumor cells spiked into healthy blood samples, and shows promise for CTC detection in medical diagnostics.
