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利用羧化纳米磁株抽提微量DNA及荧光定量检测方法的研究 被引量:1
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作者 马勇杰 古宏晨 郑伟明 《生物医学工程学杂志》 EI CAS CSCD 北大核心 2007年第5期1093-1096,共4页
利用实验室制备的表面修饰有羧基的Fe3O4纳米磁珠对DNA的分离进行了研究,确立了快速、高效、安全、经济的分离纯化方法。利用荧光染料Sybr GreenⅠ对微量DNA进行定量检测,具有灵敏度高、重复性好及操作简单的优点。
关键词 羧基 纳米磁株 循环DNA SYBR GreenⅠ
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Magnetic iron oxide nanoparticles carrying PTEN gene to reverse cisplatin-resistance of A549/CDDP cell lines
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作者 闵凌峰 何玲玲 +2 位作者 陈琼 俞巧 谢明萱 《Journal of Central South University》 SCIE EI CAS 2012年第2期331-339,共9页
To evaluate the feasibility of using magnetic iron oxide nanoparticle as wild PTEN gene carrier for transfection in vitro to reverse cisplatin-resistance of A549/CDDP cells, A549/CDDP cells were transfected with the w... To evaluate the feasibility of using magnetic iron oxide nanoparticle as wild PTEN gene carrier for transfection in vitro to reverse cisplatin-resistance of A549/CDDP cells, A549/CDDP cells were transfected with the wild PTEN gene expression plasmid (pGFP-PTEN) by magnetic iron nanoparticle and lipo2000. The transfection efficiency was detected by fluorescence microscope and flow cytometer. The expression levels of PTEN mRNA and protein were detected by reverse transcription polymerase chain reaction (RT-PCR) and immunocytochemistry analysis. The effect of PTEN transfection on cell cycle enhances the sensitivity of A549/CDDP to cisplatin and nanoparticle-mediated transfection has a higher efficiency than that of the liposome-mediated group. The apoptosis level was up-regulated in PTEN transfection group. The magnetic iron oxide nanoparticle could be used as one of the ideal gene carriers for PTEN gene delivery in vitro. PTEN can be an effective target for reversing cisplatin-resistance in lung cancer. 展开更多
关键词 magnetic iron oxide nanoparticle liposome transfection lung cancer PTEN cisplatin-resistance
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