目的检测重组人骨形成蛋白2(recombinant human bone morphogenetic protein2,rhBMP-2)与纳米羟基磷灰石/胶原/聚乳酸(nano-hydroxyapatite/collagen/polylactic acid,nHAC/PLA)复合后形成的活性nHAC/PLA(active nHAC/PLA,AnHAC/PLA)修...目的检测重组人骨形成蛋白2(recombinant human bone morphogenetic protein2,rhBMP-2)与纳米羟基磷灰石/胶原/聚乳酸(nano-hydroxyapatite/collagen/polylactic acid,nHAC/PLA)复合后形成的活性nHAC/PLA(active nHAC/PLA,AnHAC/PLA)修复颅骨极限缺损的效果。方法新西兰大白兔48只,体重2.0～2.5kg。制备直径为15mm的颅骨全层缺损模型,随机分成4组,每组12只。阳性对照组:缺损区植入自体髂骨;空白对照组:缺损区不植入任何材料;阴性对照组:缺损区植入nHAC/PLA;实验组:缺损区植入AnHAC/PLA,每块AnHAC/PLA上平均吸附rhBMP-21.431mg。术后8、16周通过比较颅骨缺损区X线阻射面积占总缺损面积百分比、HE染色和Masson's三色法染色观察颅骨极限缺损的修复情况。结果术后8、16周,各组颅骨缺损区X线阻射程度,阳性对照组分别为67.21%±2.06%、86.48%±1.73%,空白对照组分别为5.84%±1.92%、9.48%±2.72%,阴性对照组分别为19.13%±2.51%、35.67%±3.28%,实验组分别为58.84%±2.55%、85.61%±3.36%。其中除16周实验组与阳性对照组以及空白对照组8、16周比较差异无统计学意义(P>0.05)外,其余各组各时间点比较差异均有统计学意义(P<0.05)。组织学观察显示,阳性对照组骨缺损区16周骨小梁较8周增宽,被大量骨组织充填;空白对照组8、16周骨缺损区均被纤维组织充填,无新骨生成;阴性对照16周骨缺损区为剩余材料与纤维组织充填,周边新骨较8周形成增多;实验组8周材料植入区为新生骨替代,16周新生骨呈板层状,缺损区材料残留较少,且周围可见较多成骨细胞。结论nHAC/PLA是rhBMP-2的良好载体,两者复合后制备的AnHAC/PLA具有良好骨形成能力,有望应用于临床上修复较大型骨缺损。展开更多
PLA/MWNT/HA hybrid nanofibers were prepared via electrospinning technology.Multi walled carbon nanotube (MWNT) were first treated by anodic oxidation,which resulted in various functional groups such as C—O,CO and O—...PLA/MWNT/HA hybrid nanofibers were prepared via electrospinning technology.Multi walled carbon nanotube (MWNT) were first treated by anodic oxidation,which resulted in various functional groups such as C—O,CO and O—CO on the surface of oxidized MWNT.Then the MWNT/hydroxyapatite (HA) nano composites (MWNT content 3?wt%) were in situ synthesized by wet method with the help of ultrasonic treatment.Because of the strong interaction between the functional groups on the surface of MWNT and Ca 2+ in HA,the obtained HA nano particles were deposited onto the surface of oxidized MWNT.The as received MWNT/HA nano composites with excellent biocompatibility and osteoinduction were introduced into a bioabsorbable polymer,polylactide (PLA),and the PLA/MWNT/HA hybrid nanofibers were electrospun through a double solvent method under the voltage of 18 kV and the capillary to target distance was 4?cm.The structure and morphology of hybrid nanofibers were characterized by SEM.Because of the nanoscaled diameters,microscaled interconnected pores and suitable surface chemistry,the hybrid nanofibers would match the requirements of ideal bone tissue engineering scaffold and may be a potential material for preparing bone tissue engineering scaffold.展开更多
文摘目的检测重组人骨形成蛋白2(recombinant human bone morphogenetic protein2,rhBMP-2)与纳米羟基磷灰石/胶原/聚乳酸(nano-hydroxyapatite/collagen/polylactic acid,nHAC/PLA)复合后形成的活性nHAC/PLA(active nHAC/PLA,AnHAC/PLA)修复颅骨极限缺损的效果。方法新西兰大白兔48只,体重2.0～2.5kg。制备直径为15mm的颅骨全层缺损模型,随机分成4组,每组12只。阳性对照组:缺损区植入自体髂骨;空白对照组:缺损区不植入任何材料;阴性对照组:缺损区植入nHAC/PLA;实验组:缺损区植入AnHAC/PLA,每块AnHAC/PLA上平均吸附rhBMP-21.431mg。术后8、16周通过比较颅骨缺损区X线阻射面积占总缺损面积百分比、HE染色和Masson's三色法染色观察颅骨极限缺损的修复情况。结果术后8、16周,各组颅骨缺损区X线阻射程度,阳性对照组分别为67.21%±2.06%、86.48%±1.73%,空白对照组分别为5.84%±1.92%、9.48%±2.72%,阴性对照组分别为19.13%±2.51%、35.67%±3.28%,实验组分别为58.84%±2.55%、85.61%±3.36%。其中除16周实验组与阳性对照组以及空白对照组8、16周比较差异无统计学意义(P>0.05)外,其余各组各时间点比较差异均有统计学意义(P<0.05)。组织学观察显示,阳性对照组骨缺损区16周骨小梁较8周增宽,被大量骨组织充填;空白对照组8、16周骨缺损区均被纤维组织充填,无新骨生成;阴性对照16周骨缺损区为剩余材料与纤维组织充填,周边新骨较8周形成增多;实验组8周材料植入区为新生骨替代,16周新生骨呈板层状,缺损区材料残留较少,且周围可见较多成骨细胞。结论nHAC/PLA是rhBMP-2的良好载体,两者复合后制备的AnHAC/PLA具有良好骨形成能力,有望应用于临床上修复较大型骨缺损。
文摘PLA/MWNT/HA hybrid nanofibers were prepared via electrospinning technology.Multi walled carbon nanotube (MWNT) were first treated by anodic oxidation,which resulted in various functional groups such as C—O,CO and O—CO on the surface of oxidized MWNT.Then the MWNT/hydroxyapatite (HA) nano composites (MWNT content 3?wt%) were in situ synthesized by wet method with the help of ultrasonic treatment.Because of the strong interaction between the functional groups on the surface of MWNT and Ca 2+ in HA,the obtained HA nano particles were deposited onto the surface of oxidized MWNT.The as received MWNT/HA nano composites with excellent biocompatibility and osteoinduction were introduced into a bioabsorbable polymer,polylactide (PLA),and the PLA/MWNT/HA hybrid nanofibers were electrospun through a double solvent method under the voltage of 18 kV and the capillary to target distance was 4?cm.The structure and morphology of hybrid nanofibers were characterized by SEM.Because of the nanoscaled diameters,microscaled interconnected pores and suitable surface chemistry,the hybrid nanofibers would match the requirements of ideal bone tissue engineering scaffold and may be a potential material for preparing bone tissue engineering scaffold.
