Stimulated emission depletion(STED) microscope is one of the most prominent super-resolution bio-imaging instruments, which holds great promise for ultrahigh-resolution imaging of cells. To construct a STED microscope...Stimulated emission depletion(STED) microscope is one of the most prominent super-resolution bio-imaging instruments, which holds great promise for ultrahigh-resolution imaging of cells. To construct a STED microscope, it is challenging to realize temporal synchronization between the excitation pulses and the depletion pulses. In this study, we present a simple and low-cost method to achieve pulse synchronization by using a condensed fluorescent dye as a depletion indicator. By using this method, almost all the confocal microscopes can be upgraded to a STED system without losing its original functions. After the pulse synchronization,our STED system achieved sub-100-nm resolution for fluorescent nanospheres and single-cell imaging.展开更多
Here we report a new approach referred as ＂soft- to-hard templating＂ strategy via the copolymerization of carbon source （dopamine） and silica source （tetraethyl orthosilicate） for the synthesis of well dispersed...Here we report a new approach referred as ＂soft- to-hard templating＂ strategy via the copolymerization of carbon source （dopamine） and silica source （tetraethyl orthosilicate） for the synthesis of well dispersed N-doped mesoporous carbon nanospheres （MCNs）, which exhibit high performance for electrochemical supercapacitor. This method overcomes the shortcoming of uncontrolled dis- persity and complicated procedures of soft- or hard-tem- plating methods, respectively. Moreover, the synthesized MCNs feature enriched heteroatom N-doping and easy functionalization by noble-metal nanoparticles during the one-pot synthesis. All the above characters make the as- prepared MCNs a promising platform in a variety of applications. To demonstrate the applicability of the syn- thesized nitrogen-doped MCNs, this material has been employed as an electrode for high-performance electro- chemical supercapacitor, which shows a capacitance of 223 and 140 F/g at current densities of 0.5 and 10 A/g in 1 mol/L KOH electrolyte, respectively.展开更多
基金supported by the National Natural Science Foundation of China (21227804, 21390414, 61378062, 21505148)National Key Research and Development Program (2016YFA0400902)the Natural Science Foundation of Shanghai (15ZR1448400, 14ZR1448000)
文摘Stimulated emission depletion(STED) microscope is one of the most prominent super-resolution bio-imaging instruments, which holds great promise for ultrahigh-resolution imaging of cells. To construct a STED microscope, it is challenging to realize temporal synchronization between the excitation pulses and the depletion pulses. In this study, we present a simple and low-cost method to achieve pulse synchronization by using a condensed fluorescent dye as a depletion indicator. By using this method, almost all the confocal microscopes can be upgraded to a STED system without losing its original functions. After the pulse synchronization,our STED system achieved sub-100-nm resolution for fluorescent nanospheres and single-cell imaging.
基金This work was supported by the National Basic Research Program of China （2013CB933200）, the National High Technology Research and Development Program of China （2012AA062703）, the National Natural Science Foundation of China （21177137） and the Youth Innovation Promotion Association CAS （2012200）.
文摘Here we report a new approach referred as ＂soft- to-hard templating＂ strategy via the copolymerization of carbon source （dopamine） and silica source （tetraethyl orthosilicate） for the synthesis of well dispersed N-doped mesoporous carbon nanospheres （MCNs）, which exhibit high performance for electrochemical supercapacitor. This method overcomes the shortcoming of uncontrolled dis- persity and complicated procedures of soft- or hard-tem- plating methods, respectively. Moreover, the synthesized MCNs feature enriched heteroatom N-doping and easy functionalization by noble-metal nanoparticles during the one-pot synthesis. All the above characters make the as- prepared MCNs a promising platform in a variety of applications. To demonstrate the applicability of the syn- thesized nitrogen-doped MCNs, this material has been employed as an electrode for high-performance electro- chemical supercapacitor, which shows a capacitance of 223 and 140 F/g at current densities of 0.5 and 10 A/g in 1 mol/L KOH electrolyte, respectively.
