通过体外细胞实验,研究透明质酸钠/植物鞘氨醇/三肽-1经皮共输送纳米载体对皮肤屏障的修复作用。采用细胞实验考察透明质酸钠/植物鞘氨醇/三肽-1纳米载体对角质形成细胞(Ha Ca T)的增殖能力,细胞迁移能力,细胞分泌丝聚蛋白、水通道蛋白...通过体外细胞实验,研究透明质酸钠/植物鞘氨醇/三肽-1经皮共输送纳米载体对皮肤屏障的修复作用。采用细胞实验考察透明质酸钠/植物鞘氨醇/三肽-1纳米载体对角质形成细胞(Ha Ca T)的增殖能力,细胞迁移能力,细胞分泌丝聚蛋白、水通道蛋白3及紧密连接蛋白-1水平的影响。结果表明,与游离活性物比较,透明质酸钠/植物鞘氨醇/三肽-1纳米载体能显著提高Ha Ca T细胞增殖能力(P <0.05),增加Ha Ca T细胞迁移能力(P <0.01),且能显著促进HaCaT细胞分泌丝聚蛋白、水通道蛋白3及紧密连接蛋白-1水平(P <0.05)。说明透明质酸钠/植物鞘氨醇/三肽-1纳米载体具有修复皮肤屏障的作用,在新型高性能皮肤屏障修复护肤品领域具有良好的应用前景。展开更多
Owing to the importance of drug delivery in cancer or other diseases' therapy, the targeted drug delivery (TDD) system has been attracting enormous interest. Herein, we model the TDD system and design a novel rod-...Owing to the importance of drug delivery in cancer or other diseases' therapy, the targeted drug delivery (TDD) system has been attracting enormous interest. Herein, we model the TDD system and design a novel rod-like nanocarrier by using the coarse grained model-based density functional theory, which combines a modified fundamental measure theory for the excluded-volume effects, Wertheim's first-order thermodynamics perturbation theory for the chain connectivity and the mean field approximation for van der Waals attraction. For comparison, the monomer nanocarrier TDD system and the no nanocarrier one are also investigated. The results indicate that the drug delivery capacity of rod-like nanocarriers is about 62 times that of the no nanocarrier one, and about 6 times that of the monomer nanocarriers. The reason is that the rod-like nanocarriers would self-assemble into the smectic phase perpendicular to the membrane surface. It is the self-assembly of the rod-like nanocarriers that yields the driving force for the targeted delivery of drugs inside the cell membrane. By contrast, the conventional monomer nanocarrier drug delivery system lacks the driving force to deliver the drugs into the cell membrane. In short, the novel rod-like nanocarrier TDD system may improve the drug delivery efficiency. Although the model in this work is simple, it is expected that the system may provide a new perspective for cancer targeted therapy.展开更多
文摘通过体外细胞实验,研究透明质酸钠/植物鞘氨醇/三肽-1经皮共输送纳米载体对皮肤屏障的修复作用。采用细胞实验考察透明质酸钠/植物鞘氨醇/三肽-1纳米载体对角质形成细胞(Ha Ca T)的增殖能力,细胞迁移能力,细胞分泌丝聚蛋白、水通道蛋白3及紧密连接蛋白-1水平的影响。结果表明,与游离活性物比较,透明质酸钠/植物鞘氨醇/三肽-1纳米载体能显著提高Ha Ca T细胞增殖能力(P <0.05),增加Ha Ca T细胞迁移能力(P <0.01),且能显著促进HaCaT细胞分泌丝聚蛋白、水通道蛋白3及紧密连接蛋白-1水平(P <0.05)。说明透明质酸钠/植物鞘氨醇/三肽-1纳米载体具有修复皮肤屏障的作用,在新型高性能皮肤屏障修复护肤品领域具有良好的应用前景。
基金supported by the National Natural Science Foundation of China (20874005, 20736002, 20821004)the National Basic Research Program of China (2011CB706900)+1 种基金Huo Yingdong Fundamental Research Foundation (121070)Novel Team (IRT0807) from Ministry of Education and the Chemical Grid Project of BUCT
文摘Owing to the importance of drug delivery in cancer or other diseases' therapy, the targeted drug delivery (TDD) system has been attracting enormous interest. Herein, we model the TDD system and design a novel rod-like nanocarrier by using the coarse grained model-based density functional theory, which combines a modified fundamental measure theory for the excluded-volume effects, Wertheim's first-order thermodynamics perturbation theory for the chain connectivity and the mean field approximation for van der Waals attraction. For comparison, the monomer nanocarrier TDD system and the no nanocarrier one are also investigated. The results indicate that the drug delivery capacity of rod-like nanocarriers is about 62 times that of the no nanocarrier one, and about 6 times that of the monomer nanocarriers. The reason is that the rod-like nanocarriers would self-assemble into the smectic phase perpendicular to the membrane surface. It is the self-assembly of the rod-like nanocarriers that yields the driving force for the targeted delivery of drugs inside the cell membrane. By contrast, the conventional monomer nanocarrier drug delivery system lacks the driving force to deliver the drugs into the cell membrane. In short, the novel rod-like nanocarrier TDD system may improve the drug delivery efficiency. Although the model in this work is simple, it is expected that the system may provide a new perspective for cancer targeted therapy.
