基于纳米金粒子比色法的汞离子检测方法

提出了一种基于纳米金粒子比色法的汞离子检测方法,采用铋试剂Ⅱ修饰的纳米金粒子胶体悬浮液检测汞离子。汞离子与铋试剂Ⅱ选择性配位,使纳米金粒子胶体悬浮液反团聚,汞离子浓度与溶液吸光度呈一定的线性关系,线性范围为0.05μg/L^1 mg/L,相对标准偏差为1.98%~3.52%。在实验条件下,采用SavitzkyGolay一阶导数对连续投影算法提取的特征波长进行了预处理,通过线性支持向量机回归模型进行建模与分析,该模型具有良好的自预测能力和实际预测能力。校正集的相关系数为0.9685,均方根误差为0.0412 mg/L,验证集的相关系数为0.9600,均方根误差为0.0434 mg/L。本文方法为提高汞离子浓度的检测效率提供了有效支持。

Utilization of unmodified gold nanoparticles in colorimetric detection

This review begins with an overview of the appealing properties and various applications of gold nanoparticles, and briefly summarizes recent advances in using unmodified gold nanoparticles to detect different kinds of targets including nucleic acids, proteins, metal ions and small organic molecules. The key point to the unmodified gold nanoparticle-based visual detection assay is to control dispersion and aggregation of colloidal nanoparticles by targets of interest, which usually relies on affinities between gold nanoparticles and targets. The degree of dispersion or aggregation can be visualized through the change of the solution color or the precipitation of nanoparticles from the solution. Thus, the existence of the target molecules can be trans-lated into optical signals and monitored by the naked eye conveniently. Finally, some future prospects of this research field are given.