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用聚合酶链式反应(PCR)检测马铃薯纺锤块茎类病毒 被引量:9
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作者 何小源 周广和 +2 位作者 成卓敏 王立阳 陈剑波 《中国病毒学》 CSCD 1992年第3期362-366,共5页
用DNA合成仪合成两个马铃薯纺锤块茎类病毒(Potato spindle tuber viroid, PSTVd)特异性引物,从感病的马铃薯块茎组织的核酸抽提液中,用反转录酶合成PSTVd eDNA,然后用PCR法进行扩增,扩增产物用电泳检测,建立了用PCR法检测PSTVd的新方... 用DNA合成仪合成两个马铃薯纺锤块茎类病毒(Potato spindle tuber viroid, PSTVd)特异性引物,从感病的马铃薯块茎组织的核酸抽提液中,用反转录酶合成PSTVd eDNA,然后用PCR法进行扩增,扩增产物用电泳检测,建立了用PCR法检测PSTVd的新方法。结果表明,该方法特异性强,灵敏度可达0.15pg,比现有其它检测方法高,而且样品用量少。 展开更多
关键词 马铃薯 纺锤块类病毒 聚合酶链反应
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Detection of Potato Spindle Tuber Viroid, and Its Impact on Growth, Production of Tomatoes in Greenhouse
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作者 Haniyeh Mohamed Algamodi Jaber Abdullah Khalil +1 位作者 Taher Ahmed Abouhliqh Khaled Ojdj 《Journal of Agricultural Science and Technology(B)》 2012年第8期940-949,共10页
The viability of most tomato varieties cultivated in Libya have been tested to infect with potato spindle tuber viroid/potatoes (PSTVd) and its impact on growth and production of some of these varieties, which were ... The viability of most tomato varieties cultivated in Libya have been tested to infect with potato spindle tuber viroid/potatoes (PSTVd) and its impact on growth and production of some of these varieties, which were mechanically inoculated with Libyan isolate of viroid PSTVd as follows: Vlkato, Sankarh, Lebda, Jasmine, Kenza and Hana. The percent of incidence were 95.95%, 90%, 90.80%, 80% and 20%, respectively. The following varieties have been contagious mechanically with viroid of PSTVd: Vlkato, zahra, Toria, Lebda, Hoda, Farwa, Alkaraz, Naziha, Rim Star and Kartika. The percent of incidence were 95.95%, 85%, 85.80%, 80%, 70.40%, 0.0%, 0.0%, respectively. The varied symptoms of wrinkle, twist, warp, swell the veins of the leaves, dark brown spots formation and a large yellow spots turned into white patches. Also the effect of the Egyptian isolate viroid PSTVd in the growth and production of varieties Jasmine, Lebda, Soberhalim, and treasure No. 185 had been studied, as the average rates of decline in the production of the fruits tomatoes/tomato 43.4% and 17% length of plants, and in the fresh weight and dry root of the sum of 35% and 37% respictively. 展开更多
关键词 Potato spindle tuber viroid the infection of tomato plants nucleic acid PCR.
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