The peritrophic membrane plays an important role in the defense system of the arthropod gut. The digestive tract is considered one of the major tissues targeted by white spot syndrome virus （WSSV） in shrimp. In this...The peritrophic membrane plays an important role in the defense system of the arthropod gut. The digestive tract is considered one of the major tissues targeted by white spot syndrome virus （WSSV） in shrimp. In this study, the nucleotide sequence encoding peritrophin-like protein of Litopenaeus vannamei （LvPT） was amplified from a yeast two-hybrid library of L. vannamei. The epitope peptide of LvPT was predicted with the GenScript OptimumAntigenTM design tool. An anti-LvPT polyclonal antibody was produced and shown to specifically bind a band at -27 kDa, identified as LvPT. The LvPT protein was expressed and its concentration determined. LvPT dsRNA （4 pg per shrimp） was used to inhibit LvPT expression in shrimp, and a WSSV challenge experiment was then performed with reverse gavage. The pleopods, stomachs, and guts were collected from the shrimp at 0, 24, 48, and 72 h post-infection （hpi）. Viral load quantification showed that the levels of WSSV were significantly lower in the pleopods, stomachs, and guts of shrimp after LvPT dsRNA interference than in those of the controls at 48 and 72 hpi. Our results imply that LvPT plays an important role during WSSV infection of the digestive tract.展开更多
This paper proposes a parameter determination method of distribution voltage regulators load ratio control transformers （LRT） and step voltage regulators （SVR） considering the tap change and voltage profile. The m...This paper proposes a parameter determination method of distribution voltage regulators load ratio control transformers （LRT） and step voltage regulators （SVR） considering the tap change and voltage profile. The method takes two procedures in order to simplify the optimization problem and to reduce calculation time. One is to simultaneously determine the control parameters of LRT and SVR minimizing voltage violations and voltage variations. The algorithm is based on particle swarm optimization （PSO）, which is one of non-linear optimization methods by using a concept of swarm intelligence. Another is to determine the dead-band width of LRT and SVR on the basis of bi-evaluation of tap change and voltage margin. The concept of a Pareto optimal solution is used for the decision of the best dead-band width. As the results of numerical simulations using distribution network model, the validity of the proposed method has been affirmed.展开更多
基金Supported by the National Basic Research Program of China(973 Program)(No.2012CB114403)the China Agriculture Research System-47(CARS-47)
文摘The peritrophic membrane plays an important role in the defense system of the arthropod gut. The digestive tract is considered one of the major tissues targeted by white spot syndrome virus （WSSV） in shrimp. In this study, the nucleotide sequence encoding peritrophin-like protein of Litopenaeus vannamei （LvPT） was amplified from a yeast two-hybrid library of L. vannamei. The epitope peptide of LvPT was predicted with the GenScript OptimumAntigenTM design tool. An anti-LvPT polyclonal antibody was produced and shown to specifically bind a band at -27 kDa, identified as LvPT. The LvPT protein was expressed and its concentration determined. LvPT dsRNA （4 pg per shrimp） was used to inhibit LvPT expression in shrimp, and a WSSV challenge experiment was then performed with reverse gavage. The pleopods, stomachs, and guts were collected from the shrimp at 0, 24, 48, and 72 h post-infection （hpi）. Viral load quantification showed that the levels of WSSV were significantly lower in the pleopods, stomachs, and guts of shrimp after LvPT dsRNA interference than in those of the controls at 48 and 72 hpi. Our results imply that LvPT plays an important role during WSSV infection of the digestive tract.
文摘This paper proposes a parameter determination method of distribution voltage regulators load ratio control transformers （LRT） and step voltage regulators （SVR） considering the tap change and voltage profile. The method takes two procedures in order to simplify the optimization problem and to reduce calculation time. One is to simultaneously determine the control parameters of LRT and SVR minimizing voltage violations and voltage variations. The algorithm is based on particle swarm optimization （PSO）, which is one of non-linear optimization methods by using a concept of swarm intelligence. Another is to determine the dead-band width of LRT and SVR on the basis of bi-evaluation of tap change and voltage margin. The concept of a Pareto optimal solution is used for the decision of the best dead-band width. As the results of numerical simulations using distribution network model, the validity of the proposed method has been affirmed.
