To reduce the difficulty of implementation and shorten the runtime of the traditional Kim-Fisher model, an entirely discrete Kim-Fisher-like model on lattices is proposed. The discrete model is directly built on the l...To reduce the difficulty of implementation and shorten the runtime of the traditional Kim-Fisher model, an entirely discrete Kim-Fisher-like model on lattices is proposed. The discrete model is directly built on the lattices, and the greedy algorithm is used in the implementation to continually decrease the energy function. First, regarding the gray values in images as discrete-valued random variables makes it possible to make a much simpler estimation of conditional entropy. Secondly, a uniform method within the level set framework for two-phase and multiphase segmentations without extension is presented. Finally, a more accurate approximation to the curve length on lattices with multi-labels is proposed. The experimental results show that, compared with the continuous Kim-Fisher model, the proposed model can obtain comparative results, while the implementation is much simpler and the runtime is dramatically reduced.展开更多
[Objective] This study aimed to optimize the chromatographic conditions for detecting ellagic acid in pomegranate peels using HPLC method. [Method] By using 0.2 mg/ml ellagic acid standard solution, on the basis of si...[Objective] This study aimed to optimize the chromatographic conditions for detecting ellagic acid in pomegranate peels using HPLC method. [Method] By using 0.2 mg/ml ellagic acid standard solution, on the basis of single-factor experiment and orthogonal experiment, chromatographic conditions (mobile phase ratio, flow rate, col- umn temperature) for detecting ellagic acid using HPLC were optimized. Based on the optimal chromatographic conditions, the ellagic acid content in experimental pomegranate peels was determined. [Resull] The optimal chromatographic conditions for detecting ellagic acid in pomegranate peels using HPLC method are: 1.2% phos- phoric acid:acetonitrile=85:15, column temperature of 35 ℃, and flow rate of 1.0 ml/min. The linear regression equation of ellagic acid is: y=2.9e+0.6x+4.4e+5 (FF=9 999). Ac- cording to the standard addition recovery test, the average recovery rate of ellagic acid is 98.20%, and RSD is 0.60%. Under above optimized chromatographic condi- tions, ellagic acid can be well separated from other interfering components in pomegranate peels, with shorter peak time and ideal effect, which is convenient for the detection in production practices. [Conclusion] This study laid the foundation for detecting ellagic acid in pomegranate peels using HPLC method.展开更多
文摘To reduce the difficulty of implementation and shorten the runtime of the traditional Kim-Fisher model, an entirely discrete Kim-Fisher-like model on lattices is proposed. The discrete model is directly built on the lattices, and the greedy algorithm is used in the implementation to continually decrease the energy function. First, regarding the gray values in images as discrete-valued random variables makes it possible to make a much simpler estimation of conditional entropy. Secondly, a uniform method within the level set framework for two-phase and multiphase segmentations without extension is presented. Finally, a more accurate approximation to the curve length on lattices with multi-labels is proposed. The experimental results show that, compared with the continuous Kim-Fisher model, the proposed model can obtain comparative results, while the implementation is much simpler and the runtime is dramatically reduced.
文摘[Objective] This study aimed to optimize the chromatographic conditions for detecting ellagic acid in pomegranate peels using HPLC method. [Method] By using 0.2 mg/ml ellagic acid standard solution, on the basis of single-factor experiment and orthogonal experiment, chromatographic conditions (mobile phase ratio, flow rate, col- umn temperature) for detecting ellagic acid using HPLC were optimized. Based on the optimal chromatographic conditions, the ellagic acid content in experimental pomegranate peels was determined. [Resull] The optimal chromatographic conditions for detecting ellagic acid in pomegranate peels using HPLC method are: 1.2% phos- phoric acid:acetonitrile=85:15, column temperature of 35 ℃, and flow rate of 1.0 ml/min. The linear regression equation of ellagic acid is: y=2.9e+0.6x+4.4e+5 (FF=9 999). Ac- cording to the standard addition recovery test, the average recovery rate of ellagic acid is 98.20%, and RSD is 0.60%. Under above optimized chromatographic condi- tions, ellagic acid can be well separated from other interfering components in pomegranate peels, with shorter peak time and ideal effect, which is convenient for the detection in production practices. [Conclusion] This study laid the foundation for detecting ellagic acid in pomegranate peels using HPLC method.