为阐明金丝猴毛首线虫线粒体细胞色素c氧化酶Ⅰ亚基(cox1)基因部分(pcox1)序列的遗传变异情况,以长沙生态动物园金丝猴体内分离的毛首线虫作为研究对象,根据cox1序列构建其与其他毛首线虫的进化发育关系。使用PCR方法扩增金丝猴毛首线...为阐明金丝猴毛首线虫线粒体细胞色素c氧化酶Ⅰ亚基(cox1)基因部分(pcox1)序列的遗传变异情况,以长沙生态动物园金丝猴体内分离的毛首线虫作为研究对象,根据cox1序列构建其与其他毛首线虫的进化发育关系。使用PCR方法扩增金丝猴毛首线虫线粒体cox1,然后将所测得的序列应用Clustal X 1.81程序进行比对,最后用Mega 5.0程序NJ法绘制种系发育树。结果显示,本实验扩增获得的6条毛首线虫分离株cox1序列长度一致,均为411 bp,种内变异为0。种系发育分析结果表明,这6条毛首线虫分离株位于同一分支。鉴于金丝猴毛首线虫的cox1基因种内保守,而种间差异大(24.0%~34.1%),因此可以作为种间鉴定检测研究的遗传标记。本研究结果为进一步研究金丝猴毛首线虫的群体遗传结构奠定了基础。展开更多
目的用形态学方法和分子生物学方法对贵州黔南地区5条带绦虫进行分类学鉴定。方法在观察虫体头节、孕节等形态的基础上,采用带绦虫线粒体细胞色素C氧化酶亚单位1(cy-tochrome c oxidase subunit 1,cox1)基因片段的特异性引物对虫体DNA进...目的用形态学方法和分子生物学方法对贵州黔南地区5条带绦虫进行分类学鉴定。方法在观察虫体头节、孕节等形态的基础上,采用带绦虫线粒体细胞色素C氧化酶亚单位1(cy-tochrome c oxidase subunit 1,cox1)基因片段的特异性引物对虫体DNA进行PCR扩增,产物经琼脂糖凝胶电泳分析。结果 5条带绦虫头节近方形,有4个吸盘,无顶突和小钩,孕节子宫每侧分支数为18～24支。以Tasia为引物的PCR扩增产物经琼脂糖凝胶电泳,均出现约260bp的条带,而以Tsag引物均未见扩增产物。结论经鉴定,所采集的4株都匀绦虫和1株独山绦虫均为亚洲带绦虫,提示贵州都匀、独山地区为亚洲带绦虫流行区。展开更多
[Objective] The research aimed at cloning and analyzing mitochondrial cytochrome oxidase I gene（cox 1）of C.suppressalis.[Method] The mitochondrial cox 1 gene of C.suppressalis was cloned with PCR method and sequence...[Objective] The research aimed at cloning and analyzing mitochondrial cytochrome oxidase I gene（cox 1）of C.suppressalis.[Method] The mitochondrial cox 1 gene of C.suppressalis was cloned with PCR method and sequenced.Then,cox1 sequences of other 21 Lepidopteran species were obtained by blasting the GenBank with cox 1 gene sequence of C.suppressalis.Finally,homology comparison and molecular phylogenitic analysis among the 22 Lepidopteran species were conducted.[Result] The open reading frame of cox 1 gene from C.suppressalis contained 1 531 nucleotides encoding a putative protein of 510 amino acids.The cox1 gene used a start codon CGA,and an incomplete termination codon composed of only T.Based on the amino acid sequences of cox 1,the molecular phylogenetic tree of Lepidoptera was reconstructed using the maximum likelihood（ML）method.The molecular phylogenetic tree was similar to the morphological phylogenetic tree mainly,but also showed some differences.[Conclusion] The result will provide reference for further research on expression and application of cox 1 gene.展开更多
Phylogenetic relationships of Pamphagidae were examined using cytochrome oxidase subunit I（cox1） gene sequences.Eleven species of Pamphagidae from 7 genera were sequenced to obtain mt DNA data,along with 2 species f...Phylogenetic relationships of Pamphagidae were examined using cytochrome oxidase subunit I（cox1） gene sequences.Eleven species of Pamphagidae from 7 genera were sequenced to obtain mt DNA data,along with 2 species from the Gen Bank nucleotide database.The results of sequence comparisons showed the cox1 gene is 1 534 bp in length,including 326 varible sites and 211 parsimonious information sites.The percentage of A ＋T is 67.1% in the nucleotide sequences,showing a strong AT bias.Genetic distances among subfamilies are 0.08.Using Locusta migratoria as outgroup,the phylogenetic trees were reconstructed with NJ,MP,ML and Bayesian inferences,and the results showed that the clustering results were approximately identical to that of classical morphological classification.Thrinchinae and Pamphaginae were a monophyletic group,respectively.Two species of the genus Asiotmethis of Prionotropisinae did not get together with other species of Prionotropisinae,classification position of Asiotmethis should be further discussed by both genetic markers and morphological features.The current genus Filchnerella of Prionotropisinae was not a monophyletic group.展开更多
文摘为阐明金丝猴毛首线虫线粒体细胞色素c氧化酶Ⅰ亚基(cox1)基因部分(pcox1)序列的遗传变异情况,以长沙生态动物园金丝猴体内分离的毛首线虫作为研究对象,根据cox1序列构建其与其他毛首线虫的进化发育关系。使用PCR方法扩增金丝猴毛首线虫线粒体cox1,然后将所测得的序列应用Clustal X 1.81程序进行比对,最后用Mega 5.0程序NJ法绘制种系发育树。结果显示,本实验扩增获得的6条毛首线虫分离株cox1序列长度一致,均为411 bp,种内变异为0。种系发育分析结果表明,这6条毛首线虫分离株位于同一分支。鉴于金丝猴毛首线虫的cox1基因种内保守,而种间差异大(24.0%~34.1%),因此可以作为种间鉴定检测研究的遗传标记。本研究结果为进一步研究金丝猴毛首线虫的群体遗传结构奠定了基础。
基金Supported by New Century Program for Excellent Talents of Ministry of Education of China(NCET-07-0251)Talents Foundation of Anhui Province(08040106803)~~
文摘[Objective] The research aimed at cloning and analyzing mitochondrial cytochrome oxidase I gene（cox 1）of C.suppressalis.[Method] The mitochondrial cox 1 gene of C.suppressalis was cloned with PCR method and sequenced.Then,cox1 sequences of other 21 Lepidopteran species were obtained by blasting the GenBank with cox 1 gene sequence of C.suppressalis.Finally,homology comparison and molecular phylogenitic analysis among the 22 Lepidopteran species were conducted.[Result] The open reading frame of cox 1 gene from C.suppressalis contained 1 531 nucleotides encoding a putative protein of 510 amino acids.The cox1 gene used a start codon CGA,and an incomplete termination codon composed of only T.Based on the amino acid sequences of cox 1,the molecular phylogenetic tree of Lepidoptera was reconstructed using the maximum likelihood（ML）method.The molecular phylogenetic tree was similar to the morphological phylogenetic tree mainly,but also showed some differences.[Conclusion] The result will provide reference for further research on expression and application of cox 1 gene.
基金Supported by National Natural Science Foundation of China(31272342)the 2ndGroup Innovative Foundation Project for Students in China in 2012(201210075004)~~
文摘Phylogenetic relationships of Pamphagidae were examined using cytochrome oxidase subunit I（cox1） gene sequences.Eleven species of Pamphagidae from 7 genera were sequenced to obtain mt DNA data,along with 2 species from the Gen Bank nucleotide database.The results of sequence comparisons showed the cox1 gene is 1 534 bp in length,including 326 varible sites and 211 parsimonious information sites.The percentage of A ＋T is 67.1% in the nucleotide sequences,showing a strong AT bias.Genetic distances among subfamilies are 0.08.Using Locusta migratoria as outgroup,the phylogenetic trees were reconstructed with NJ,MP,ML and Bayesian inferences,and the results showed that the clustering results were approximately identical to that of classical morphological classification.Thrinchinae and Pamphaginae were a monophyletic group,respectively.Two species of the genus Asiotmethis of Prionotropisinae did not get together with other species of Prionotropisinae,classification position of Asiotmethis should be further discussed by both genetic markers and morphological features.The current genus Filchnerella of Prionotropisinae was not a monophyletic group.
