Ubiquitous on earth,bacteriophages are the most abundant entities in every ecosystem,but human knowledge about them is still limited compared with that about other forms of organisms.To enrich human knowledge and prom...Ubiquitous on earth,bacteriophages are the most abundant entities in every ecosystem,but human knowledge about them is still limited compared with that about other forms of organisms.To enrich human knowledge and promote the utilization of bacteriophages,it is necessary to isolate and characterize as many as possible different bacteriophages.Here we describe the isolation of a T4-like bacteriophage IME08 and a rapid method for its genetic characterization.With this method we easily cloned a few random fragments of the bacteriophage genome.Sequence analysis of these random clones showed that bacteriophage IME08 shared the highest sequence similarity with T4-like Enterobacteria phage T4（94%identity）,JS98（95% identity）,JS10（95%identity） and RB14（94%identity） respectively,which suggested that IME08 belonged to T4-like bacteriophage genus.展开更多
Present investigation is concerned with the free vibration property of a beam with periodically variable cross-sections.For the special geometry characteristic,the beam was modelled as the combination of long equal-le...Present investigation is concerned with the free vibration property of a beam with periodically variable cross-sections.For the special geometry characteristic,the beam was modelled as the combination of long equal-length uniform Euler-Bernoulli beam segments and short equal-length uniform Timoshenko beam segments alternately.By using continuity conditions,the hybrid beam unit(ETE-B) consisting of Euler-Bernoulli beam,Timoshenko beam and Euler-Bernoulli beam in sequence was developed.Classical boundary conditions of pinned-pinned,clamped-clamped and clamped-free were considered to obtain the natural frequencies.Numerical examples of the equal-length composite beam with 1,2 and 3 ETE-B units were presented and compared with the equal-length and equal-cross-section Euler-Bernoulli beam,respectively.The work demonstrates that natural frequencies of the composite beam are larger than those of the Euler-Bernoulli beam,which in practice,is the interpretation that the inner-welded plate can strengthen a hollow beam.In this work,comparisons with the finite element calculation were presented to validate the ETE-B model.展开更多
Crimean-Congo hemorrhagic fever (CCHF) is a severe illness with high fatality.Cases are reported in several countries in Africa,Europe,the Middle East,and Asia.Phylogenetic analyses based on the virus S (nucleocapsid)...Crimean-Congo hemorrhagic fever (CCHF) is a severe illness with high fatality.Cases are reported in several countries in Africa,Europe,the Middle East,and Asia.Phylogenetic analyses based on the virus S (nucleocapsid),M (glycoprotein),and L (polymerase) genome segments sequences indicate distinct geographic lineages exist but their specific genetic characteristics require elucidation.In this work we collected all full length S segment sequences and generated a phylogenetic tree based on the alignment of these 62 samples.We then analyzed the alignment using entries from AAIndex,the Amino Acid Index database,to identify amino acid mutations that performed significant changes in charge,pka,hydropathy and side chain volume.Finally,we mapped these changes back to the tree and alignment to identify correlated mutations or sites that characterized a specific lineage.Based on this analysis we are able to propose a number of sites that appear to be important for virus function and which would be good candidates for experimental mutational analysis studies.展开更多
The fast growth of Internet has cre-ated the need for high-speed switches. Re-cently, the crosspoint-queue switch has at-tracted attention because of its scalability and high performance. However, the Cross-point-Queu...The fast growth of Internet has cre-ated the need for high-speed switches. Re-cently, the crosspoint-queue switch has at-tracted attention because of its scalability and high performance. However, the Cross-point-Queue switch does not perform well under non-uniform traffic. To overcome this limitation, the Load-Balanced Cross-point-Queued switch architecture has been proposed. In this architecture, a load-balance stage is placed ahead of the Cross-point-Queued stage. The load-balance stage transforms the incoming non-uniform traffic into nearly uniform traffic at the input port of the second stage. To avoid out-of-order cells, this stage employs flow-based queues in each crosspoint buffer. Analysis and simulation results reveal that under non-uniform traffic, this new switch architecture achieves a delay performance similar to that of the Out-put-Queued switch without the need for inter- nal acceleration. In addition, its throughput is much better than that of the pure cross- point-queued switch. Finally, it can achieve the same packet loss rate as the cross- point-queue switch, while using a buffer size that is only 65% of that used by the cross- point-queue switch.展开更多
Smart grids are expected to become an essential component of the future energy system. The technical potential of smart grids is far reaching and increasingly well understood, and smart grids are now in the early phas...Smart grids are expected to become an essential component of the future energy system. The technical potential of smart grids is far reaching and increasingly well understood, and smart grids are now in the early phases of market deployment in several regions, particularly, in Europe and the US. Less understood than the technical aspects is how and to what degree end users （i.e. the customers） are willing and able to embrace smart grid technologies and the changes in mindset associated with this transition. This article reports the main findings from an lEA （International Energy Agency）-DSM （demand side management） project addressing the role of customers in a smart grid deployment scheme, specifically how customer behavior may restrict the technical potential of smart grids from being realized. With a model of household energy behavior as the theoretical point of departure, the research builds on experiences from various smart grid pilot studies, together with consumer research within similar domains, to identify behavioral challenges that are likely to hamper adoption of＂smart grid behaviors＂. Based on this insight, a set of recommendations to minimize customer resistance to smart grid deployment is suggested.展开更多
Synthetic biology is a newly developed field of research focused on designing and rebuilding novel biomolecular components, circuits, and networks. Synthetic biology can also help understand biological principles and ...Synthetic biology is a newly developed field of research focused on designing and rebuilding novel biomolecular components, circuits, and networks. Synthetic biology can also help understand biological principles and engineer complex artificial metabolic systems. DNA manipulation on a large genome-wide scale is an inevitable challenge, but a necessary tool for synthetic biology. To improve the methods used for the synthesis of long DNA fragments, here we constructed a novel shuttle vector named p GF(plasmid Genome Fast) for DNA assembly in vivo. The BAC plasmid p CC1 BAC, which can accommodate large DNA molecules, was chosen as the backbone. The sequence of the yeast artificial chromosome(YAC) regulatory element CEN6-ARS4 was synthesized and inserted into the plasmid to enable it to replicate in yeast. The selection sequence HIS3, obtained by polymerase chain reaction(PCR) from the plasmid p BS313, was inserted for screening. This new synthetic shuttle vector can mediate the transformation-associated recombination(TAR) assembly of large DNA fragments in yeast, and the assembled products can be transformed into Escherichia coli for further amplification. We also conducted in vivo DNA assembly using p GF and yeast homologous recombination and constructed a 31-kb long DNA sequence from the cyanophage PP genome. Our findings show that this novel shuttle vector would be a useful tool for efficient genome-scale DNA reconstruction.展开更多
文摘Ubiquitous on earth,bacteriophages are the most abundant entities in every ecosystem,but human knowledge about them is still limited compared with that about other forms of organisms.To enrich human knowledge and promote the utilization of bacteriophages,it is necessary to isolate and characterize as many as possible different bacteriophages.Here we describe the isolation of a T4-like bacteriophage IME08 and a rapid method for its genetic characterization.With this method we easily cloned a few random fragments of the bacteriophage genome.Sequence analysis of these random clones showed that bacteriophage IME08 shared the highest sequence similarity with T4-like Enterobacteria phage T4（94%identity）,JS98（95% identity）,JS10（95%identity） and RB14（94%identity） respectively,which suggested that IME08 belonged to T4-like bacteriophage genus.
基金Projects(51605138,U1508210)supported by the National Natural Science Foundation of ChinaProject(BK20160286)supported by the Natural Science Foundation of Jiangsu Province,ChinaProject(2015B30214)supported by the Fundamental Research Funds for the Central Universities,China
文摘Present investigation is concerned with the free vibration property of a beam with periodically variable cross-sections.For the special geometry characteristic,the beam was modelled as the combination of long equal-length uniform Euler-Bernoulli beam segments and short equal-length uniform Timoshenko beam segments alternately.By using continuity conditions,the hybrid beam unit(ETE-B) consisting of Euler-Bernoulli beam,Timoshenko beam and Euler-Bernoulli beam in sequence was developed.Classical boundary conditions of pinned-pinned,clamped-clamped and clamped-free were considered to obtain the natural frequencies.Numerical examples of the equal-length composite beam with 1,2 and 3 ETE-B units were presented and compared with the equal-length and equal-cross-section Euler-Bernoulli beam,respectively.The work demonstrates that natural frequencies of the composite beam are larger than those of the Euler-Bernoulli beam,which in practice,is the interpretation that the inner-welded plate can strengthen a hollow beam.In this work,comparisons with the finite element calculation were presented to validate the ETE-B model.
文摘Crimean-Congo hemorrhagic fever (CCHF) is a severe illness with high fatality.Cases are reported in several countries in Africa,Europe,the Middle East,and Asia.Phylogenetic analyses based on the virus S (nucleocapsid),M (glycoprotein),and L (polymerase) genome segments sequences indicate distinct geographic lineages exist but their specific genetic characteristics require elucidation.In this work we collected all full length S segment sequences and generated a phylogenetic tree based on the alignment of these 62 samples.We then analyzed the alignment using entries from AAIndex,the Amino Acid Index database,to identify amino acid mutations that performed significant changes in charge,pka,hydropathy and side chain volume.Finally,we mapped these changes back to the tree and alignment to identify correlated mutations or sites that characterized a specific lineage.Based on this analysis we are able to propose a number of sites that appear to be important for virus function and which would be good candidates for experimental mutational analysis studies.
文摘The fast growth of Internet has cre-ated the need for high-speed switches. Re-cently, the crosspoint-queue switch has at-tracted attention because of its scalability and high performance. However, the Cross-point-Queue switch does not perform well under non-uniform traffic. To overcome this limitation, the Load-Balanced Cross-point-Queued switch architecture has been proposed. In this architecture, a load-balance stage is placed ahead of the Cross-point-Queued stage. The load-balance stage transforms the incoming non-uniform traffic into nearly uniform traffic at the input port of the second stage. To avoid out-of-order cells, this stage employs flow-based queues in each crosspoint buffer. Analysis and simulation results reveal that under non-uniform traffic, this new switch architecture achieves a delay performance similar to that of the Out-put-Queued switch without the need for inter- nal acceleration. In addition, its throughput is much better than that of the pure cross- point-queued switch. Finally, it can achieve the same packet loss rate as the cross- point-queue switch, while using a buffer size that is only 65% of that used by the cross- point-queue switch.
文摘Smart grids are expected to become an essential component of the future energy system. The technical potential of smart grids is far reaching and increasingly well understood, and smart grids are now in the early phases of market deployment in several regions, particularly, in Europe and the US. Less understood than the technical aspects is how and to what degree end users （i.e. the customers） are willing and able to embrace smart grid technologies and the changes in mindset associated with this transition. This article reports the main findings from an lEA （International Energy Agency）-DSM （demand side management） project addressing the role of customers in a smart grid deployment scheme, specifically how customer behavior may restrict the technical potential of smart grids from being realized. With a model of household energy behavior as the theoretical point of departure, the research builds on experiences from various smart grid pilot studies, together with consumer research within similar domains, to identify behavioral challenges that are likely to hamper adoption of＂smart grid behaviors＂. Based on this insight, a set of recommendations to minimize customer resistance to smart grid deployment is suggested.
基金supported by the 973 program,Grant No.2012CB721102
文摘Synthetic biology is a newly developed field of research focused on designing and rebuilding novel biomolecular components, circuits, and networks. Synthetic biology can also help understand biological principles and engineer complex artificial metabolic systems. DNA manipulation on a large genome-wide scale is an inevitable challenge, but a necessary tool for synthetic biology. To improve the methods used for the synthesis of long DNA fragments, here we constructed a novel shuttle vector named p GF(plasmid Genome Fast) for DNA assembly in vivo. The BAC plasmid p CC1 BAC, which can accommodate large DNA molecules, was chosen as the backbone. The sequence of the yeast artificial chromosome(YAC) regulatory element CEN6-ARS4 was synthesized and inserted into the plasmid to enable it to replicate in yeast. The selection sequence HIS3, obtained by polymerase chain reaction(PCR) from the plasmid p BS313, was inserted for screening. This new synthetic shuttle vector can mediate the transformation-associated recombination(TAR) assembly of large DNA fragments in yeast, and the assembled products can be transformed into Escherichia coli for further amplification. We also conducted in vivo DNA assembly using p GF and yeast homologous recombination and constructed a 31-kb long DNA sequence from the cyanophage PP genome. Our findings show that this novel shuttle vector would be a useful tool for efficient genome-scale DNA reconstruction.
