A new composite two component grout comprised of modified urea-formaldehyde resin and cement was formulated to take account of the advantages and disadvantages of both the cement grout and the chem- ical grout. The ne...A new composite two component grout comprised of modified urea-formaldehyde resin and cement was formulated to take account of the advantages and disadvantages of both the cement grout and the chem- ical grout. The new grout is designed for water blocking by reinforcing as well as seepage control by bore grouting. The A component consists of a modified urea-formaldehyde resin A component, some cement, and some water. The B component is an alkaline coagulant. An orthogonal test of four factors at three lev- els showed that gel time increased with increased water content and with urea-formaldehyde resin con- tent. Gel time decreased at increased levels of alkaline coagulant. The A component of this new composite grout is stable over time. A mixed cross-over test showed that as the volume ratio of A to B increases the gel time falls at first but then increases. The solid strength decreases with increasing levels of the B com- ponent. The solid strength increases over time and becomes stable by the 28th day after mixing. The vis- cosity increases with increasing levels of resin A component. The increase is exponential and may be fit to： μ = 8.162e0.0286x.展开更多
目的:蛋白免疫印迹法是现代生物医学研究中广泛应用于蛋白定性和半定量分析的实验技术。然而,常规采用传统单一浓度凝胶的蛋白免疫印迹法在应用过程中仍有不足之处,如不能同时检测分子量很大和很小的蛋白,因而有必要探索一种增大凝胶有...目的:蛋白免疫印迹法是现代生物医学研究中广泛应用于蛋白定性和半定量分析的实验技术。然而,常规采用传统单一浓度凝胶的蛋白免疫印迹法在应用过程中仍有不足之处,如不能同时检测分子量很大和很小的蛋白,因而有必要探索一种增大凝胶有效分离范围的检测方法。本文提出采用组合凝胶来实现更大范围分子量蛋白的同时检测。方法:比较双浓度的组合凝胶与单一浓度凝胶的分离范围以及分析采用组合凝胶,蛋白免疫印迹法对大、小分子蛋白的检测效果。结果:12%/7.5%组合凝胶和15%/7.5%组合凝胶的分离范围显著大于相应的单一浓度凝胶。通过12%/7.5%组合凝胶,蛋白免疫印迹法同时检测到15-300 k Da范围内的大、小分子蛋白。结论:组合凝胶有助于蛋白免疫印迹法对分子量相差很大的蛋白进行同时检测分析,具有很强的实用性。展开更多
基金the Graduate Developing Innovation Project of Jiangsu Province of China (No. CXZZ11-0306)the Major State Basic Research and Development Program of China (No.2007CB209400)
文摘A new composite two component grout comprised of modified urea-formaldehyde resin and cement was formulated to take account of the advantages and disadvantages of both the cement grout and the chem- ical grout. The new grout is designed for water blocking by reinforcing as well as seepage control by bore grouting. The A component consists of a modified urea-formaldehyde resin A component, some cement, and some water. The B component is an alkaline coagulant. An orthogonal test of four factors at three lev- els showed that gel time increased with increased water content and with urea-formaldehyde resin con- tent. Gel time decreased at increased levels of alkaline coagulant. The A component of this new composite grout is stable over time. A mixed cross-over test showed that as the volume ratio of A to B increases the gel time falls at first but then increases. The solid strength decreases with increasing levels of the B com- ponent. The solid strength increases over time and becomes stable by the 28th day after mixing. The vis- cosity increases with increasing levels of resin A component. The increase is exponential and may be fit to： μ = 8.162e0.0286x.
文摘目的:蛋白免疫印迹法是现代生物医学研究中广泛应用于蛋白定性和半定量分析的实验技术。然而,常规采用传统单一浓度凝胶的蛋白免疫印迹法在应用过程中仍有不足之处,如不能同时检测分子量很大和很小的蛋白,因而有必要探索一种增大凝胶有效分离范围的检测方法。本文提出采用组合凝胶来实现更大范围分子量蛋白的同时检测。方法:比较双浓度的组合凝胶与单一浓度凝胶的分离范围以及分析采用组合凝胶,蛋白免疫印迹法对大、小分子蛋白的检测效果。结果:12%/7.5%组合凝胶和15%/7.5%组合凝胶的分离范围显著大于相应的单一浓度凝胶。通过12%/7.5%组合凝胶,蛋白免疫印迹法同时检测到15-300 k Da范围内的大、小分子蛋白。结论:组合凝胶有助于蛋白免疫印迹法对分子量相差很大的蛋白进行同时检测分析,具有很强的实用性。
