Intestinal microflora possesses both beneficial and pathogenic properties with respect to host health and well being. Three procedures are usually proposed for its modulation： probiotic, prebiotic and their combinati...Intestinal microflora possesses both beneficial and pathogenic properties with respect to host health and well being. Three procedures are usually proposed for its modulation： probiotic, prebiotic and their combination on symbiotic. In this in vitro study the authors assess the effect of a symbiotic combination between cellobiose and two probiotic strains （L. rhamnosus, S. thermophilus） toward two pathogenic bacteria （S. aureus and E. colO. In addition, we evaluate the influence of this fiber either on the localization of the lactic inhibiting substances and their extracelluler polymer substances （EPS） production. The results showed that the prebiotic candidate noticeably elongated the adaptation phase of the target pathogens to up than 12 hours, while stimulate the probiotic growth till 2 Log CFU/mL in favor of L. rhamnosus and to approximately 3 Log CFU/mL for S. thermophilus compared with control substrate （glucose）. The inhibiting effect was more important toward S. aureus （2.54 and 1.09 Log CFU/mL against 4.99 and 7.75 in co-culture with L. rhamnosus and S. thermophilus respectively at the 4th hour of incubation） and lesser for E. coli compared with the control substrate. And the results suggested that S thermophilus inhibited both target strains mainly by bacteriocins, whilst L. rharnnosus rather inhibited S. aureus growth by bacteriocins and E. coli ones by acids production. We also observed a significant stimulation of the exopolysaccahrids production by this fiber, which may allow a better adhesion of the probiotic candidates in the intestinal tract.展开更多
文摘Intestinal microflora possesses both beneficial and pathogenic properties with respect to host health and well being. Three procedures are usually proposed for its modulation： probiotic, prebiotic and their combination on symbiotic. In this in vitro study the authors assess the effect of a symbiotic combination between cellobiose and two probiotic strains （L. rhamnosus, S. thermophilus） toward two pathogenic bacteria （S. aureus and E. colO. In addition, we evaluate the influence of this fiber either on the localization of the lactic inhibiting substances and their extracelluler polymer substances （EPS） production. The results showed that the prebiotic candidate noticeably elongated the adaptation phase of the target pathogens to up than 12 hours, while stimulate the probiotic growth till 2 Log CFU/mL in favor of L. rhamnosus and to approximately 3 Log CFU/mL for S. thermophilus compared with control substrate （glucose）. The inhibiting effect was more important toward S. aureus （2.54 and 1.09 Log CFU/mL against 4.99 and 7.75 in co-culture with L. rhamnosus and S. thermophilus respectively at the 4th hour of incubation） and lesser for E. coli compared with the control substrate. And the results suggested that S thermophilus inhibited both target strains mainly by bacteriocins, whilst L. rharnnosus rather inhibited S. aureus growth by bacteriocins and E. coli ones by acids production. We also observed a significant stimulation of the exopolysaccahrids production by this fiber, which may allow a better adhesion of the probiotic candidates in the intestinal tract.
