Callus induction effects of nine varieties of Chenopodium quinoa Willd. were compared by taking stem segments and cotyledons of C. quinoa as the ex- plants. At the same time, callus JnductJon of stem segments was opti...Callus induction effects of nine varieties of Chenopodium quinoa Willd. were compared by taking stem segments and cotyledons of C. quinoa as the ex- plants. At the same time, callus JnductJon of stem segments was optimized, as well as the callus proliferation system. Research results showed that the optimal explant for callus induction was stem segment. The average callus induction rate of nine varieties reached 90% in culture medium MS ＋ 0.5 mg/L 2, 4-D. In the callus opti- mization test, treatment VI （MS ＋ 0.5 mg/L 2, 4-D ＋ 0.5 mg/L KT ＋ 0.5 mg/L NAA） and treatment II （MS ＋ 0.5 mg/L 2, 4-D） had close induction rate, but the callus morphology was greatly different. The latter had loose, glossy and yellowish white calluses. Therefore, culture medium MS ＋ 0.5 mg/L 2, 4-D was the optimal for callus induction. And using 2, 4-D together with KT and NAA could significantly increase the proliferation rate of calluses.展开更多
The research explored rapid multiplication on Bletilla striata and the re- suits showed that the medium of MS＋2.5 mg/L6-BA＋0.2 mg/L NAA performed the best in induction, with induction rate at 98%; the medium with MS...The research explored rapid multiplication on Bletilla striata and the re- suits showed that the medium of MS＋2.5 mg/L6-BA＋0.2 mg/L NAA performed the best in induction, with induction rate at 98%; the medium with MS＋IAA2.5mg/L＋ 2.5 mg/L6-BA＋2.0 mg/L NAA was the best in terms of induction and multiplication; the medium with 1/2MS＋0.01 mg/L NAA 1＋0.01 mg/L IAA promoted rooting.The harden- ing temperature was the optimal at 23℃-28 ℃ and relative humidity of 80%-90% the best.展开更多
[Objective] This study was conducted to acquire a large quantity of wide raspberry plantlets and provide a basis for large-scale production. [Method] Re- search was performed on series tissue culture propagation of wi...[Objective] This study was conducted to acquire a large quantity of wide raspberry plantlets and provide a basis for large-scale production. [Method] Re- search was performed on series tissue culture propagation of wide raspberry. [Re- sult] Adventitious shoots were induced with stem segments, and the best induction medium was MS ＋1.5 mg/L BA ＋0.3 mg/L NAA with an induction rate of 96%; and the best differentiation medium was MS ＋2.0 mg/L KT ＋0.5 mg/L NAA, which ex- hibited high root number and quality, with an average root number of 4.5 per plant. The substratum for training plantlets was leaf mould 60%＋perlite 30%＋vermiculite 10%; and the temperature of 23-27℃ and relative air humidity of 80%-90% were beneficial to survival of trained plantlets.[Conclusion] This study laid a foundation for further large-area cultivation of Rubus chingii Hu.展开更多
[Objective] The aim was to optimized and established the regeneration system of Moringa oleifera and provided foundation for its rapid propagation and further research. [Method] Tissue culture techniques and large sca...[Objective] The aim was to optimized and established the regeneration system of Moringa oleifera and provided foundation for its rapid propagation and further research. [Method] Tissue culture techniques and large scale micropropagation of M. oleifera were studied in this paper. By means of a series of experiments, we used the leaf of aseptic seedling from M. oleifera as explants to optimize and establish the regeneration system cultured in vitro by means of direct organogenesis. [Result] It was observed that using the fresh shelled M. oleifera seeds with 0.1% mercuric chloride for 6 minutes could reach the best disinfection effect. The seed germination rate was 85%. The leaf could produce cluster buds well using the medium with MS＋2.0 mg/L 6-BA＋0.05 mg/L NAA, while the best proliferation condition was under MS＋6-BA 1.0 mg/L＋KT 0.1 rag/L＋2, 4-D 2.0 mg/L＋NAA 0.05 mg/L. The best rooting induction culture medium was MS＋0.5 mg/L IBA, with the rooting rate as 100%. [Cenclusien] This protocol might find use in mass production of true- to-type plants and in production of transgenic plants through Agrobacterium/biolisticmediated transformation.展开更多
Amphioxus, a cephalochordate, is an important model fish for studies in evolution and comparative biology. A successful cell culture from amphioxus tissues in vitro would help understanding some basic issues. To deter...Amphioxus, a cephalochordate, is an important model fish for studies in evolution and comparative biology. A successful cell culture from amphioxus tissues in vitro would help understanding some basic issues. To determine the optimal culture conditions for proliferation of amphioxus cells, primary cultures were initiated from buccal cirri, tail, gill, gut and metapleural fold of amphioxus Branchiostoma belcheri tsingtauense. The media tested were L-15, F-12, M 199, MEM, DMEM, PRMI 1640 and LDF, each was supplemented with 20% fetal bovine serum. The optimal conditions include tail tissue cultured in L-15 or F-12 with supplement of 20% FBS and 1.5% NaCl at about 25℃.展开更多
基金Supported by National Natural Sciences Foundation of China(31301372)the Major Science and Technology Project of Zhejiang Province(2011C12030)the Haixi Prefecture science and technology project of Qinghai Province(2012-Y01)~~
文摘Callus induction effects of nine varieties of Chenopodium quinoa Willd. were compared by taking stem segments and cotyledons of C. quinoa as the ex- plants. At the same time, callus JnductJon of stem segments was optimized, as well as the callus proliferation system. Research results showed that the optimal explant for callus induction was stem segment. The average callus induction rate of nine varieties reached 90% in culture medium MS ＋ 0.5 mg/L 2, 4-D. In the callus opti- mization test, treatment VI （MS ＋ 0.5 mg/L 2, 4-D ＋ 0.5 mg/L KT ＋ 0.5 mg/L NAA） and treatment II （MS ＋ 0.5 mg/L 2, 4-D） had close induction rate, but the callus morphology was greatly different. The latter had loose, glossy and yellowish white calluses. Therefore, culture medium MS ＋ 0.5 mg/L 2, 4-D was the optimal for callus induction. And using 2, 4-D together with KT and NAA could significantly increase the proliferation rate of calluses.
基金Supported by Students’S&T Innovation Project of Zhejiang Colleges and Universities(2015R463001)~~
文摘The research explored rapid multiplication on Bletilla striata and the re- suits showed that the medium of MS＋2.5 mg/L6-BA＋0.2 mg/L NAA performed the best in induction, with induction rate at 98%; the medium with MS＋IAA2.5mg/L＋ 2.5 mg/L6-BA＋2.0 mg/L NAA was the best in terms of induction and multiplication; the medium with 1/2MS＋0.01 mg/L NAA 1＋0.01 mg/L IAA promoted rooting.The harden- ing temperature was the optimal at 23℃-28 ℃ and relative humidity of 80%-90% the best.
基金Supported by Taizhou College Students’Innovative Team Funding Project(classⅠ)~~
文摘[Objective] This study was conducted to acquire a large quantity of wide raspberry plantlets and provide a basis for large-scale production. [Method] Re- search was performed on series tissue culture propagation of wide raspberry. [Re- sult] Adventitious shoots were induced with stem segments, and the best induction medium was MS ＋1.5 mg/L BA ＋0.3 mg/L NAA with an induction rate of 96%; and the best differentiation medium was MS ＋2.0 mg/L KT ＋0.5 mg/L NAA, which ex- hibited high root number and quality, with an average root number of 4.5 per plant. The substratum for training plantlets was leaf mould 60%＋perlite 30%＋vermiculite 10%; and the temperature of 23-27℃ and relative air humidity of 80%-90% were beneficial to survival of trained plantlets.[Conclusion] This study laid a foundation for further large-area cultivation of Rubus chingii Hu.
基金National Natural Science Foundation of China(21165008)~~
文摘[Objective] The aim was to optimized and established the regeneration system of Moringa oleifera and provided foundation for its rapid propagation and further research. [Method] Tissue culture techniques and large scale micropropagation of M. oleifera were studied in this paper. By means of a series of experiments, we used the leaf of aseptic seedling from M. oleifera as explants to optimize and establish the regeneration system cultured in vitro by means of direct organogenesis. [Result] It was observed that using the fresh shelled M. oleifera seeds with 0.1% mercuric chloride for 6 minutes could reach the best disinfection effect. The seed germination rate was 85%. The leaf could produce cluster buds well using the medium with MS＋2.0 mg/L 6-BA＋0.05 mg/L NAA, while the best proliferation condition was under MS＋6-BA 1.0 mg/L＋KT 0.1 rag/L＋2, 4-D 2.0 mg/L＋NAA 0.05 mg/L. The best rooting induction culture medium was MS＋0.5 mg/L IBA, with the rooting rate as 100%. [Cenclusien] This protocol might find use in mass production of true- to-type plants and in production of transgenic plants through Agrobacterium/biolisticmediated transformation.
基金Supported by Doctoral Initial Fund of Ludong University (No.43304)
文摘Amphioxus, a cephalochordate, is an important model fish for studies in evolution and comparative biology. A successful cell culture from amphioxus tissues in vitro would help understanding some basic issues. To determine the optimal culture conditions for proliferation of amphioxus cells, primary cultures were initiated from buccal cirri, tail, gill, gut and metapleural fold of amphioxus Branchiostoma belcheri tsingtauense. The media tested were L-15, F-12, M 199, MEM, DMEM, PRMI 1640 and LDF, each was supplemented with 20% fetal bovine serum. The optimal conditions include tail tissue cultured in L-15 or F-12 with supplement of 20% FBS and 1.5% NaCl at about 25℃.
