Microsatellite markers have become one kind of the most important molecular tools used in various researches. A large number of microsatellite markers are required for the whole genome survey in the fields of molecula...Microsatellite markers have become one kind of the most important molecular tools used in various researches. A large number of microsatellite markers are required for the whole genome survey in the fields of molecular ecology,quantitative genetics and genomics. Therefore,it is extremely necessary to select several versatile,low-cost,efficient and time-and labor-saving methods to develop a large panel of microsatellite markers. In this study,we used Zhikong scallop(Chlamys farreri) as the target species to compare the efficiency of the five methods derived from three strategies for microsatellite marker development. The results showed that the strategy of constructing small insert genomic DNA library resulted in poor efficiency,while the microsatellite-enriched strategy highly improved the isolation efficiency. Although the mining public database strategy is time-and cost-saving,it is difficult to obtain a large number of microsatellite markers,mainly due to the limited sequence data of non-model species deposited in public databases. Based on the results in this study,we recommend two methods,microsatellite-enriched library construction method and FIASCO-colony hybridization method,for large-scale microsatellite marker development. Both methods were derived from the microsatellite-enriched strategy. The experimental results obtained from Zhikong scallop also provide the reference for microsatellite marker development in other species with large genomes.展开更多
The two-dimensional series patterns have been greatly applied to the changes of clothing details and edges, and the clothes with series edges is able to fully embody the clothing structure, highlight clothing modeling...The two-dimensional series patterns have been greatly applied to the changes of clothing details and edges, and the clothes with series edges is able to fully embody the clothing structure, highlight clothing modeling features, and modify the beauty of bodily form, so that clothes looks simple, decent, and cool, and its structure is clear to all; a form of rotation is formed on the human body, making the beauty-appreciation perspective of the viewers greatly changed. However, there are unending changes in the modern clothes, and therefore it is particularly important to explore a new design way of thinking in the field of design. The two-dimensional series patterns are reflected in the clothing details by relying on different composition forms, and can show the essence of traditional Chinese culture and promote design to win in details.展开更多
基金supported by ‘863’ Program (2006AA10A408 and 2006AA10A411), NSFC30571417, NYHYZX07-047, 2005DKA30470, 2006BAD09A10 and NCET-06-0594.
文摘Microsatellite markers have become one kind of the most important molecular tools used in various researches. A large number of microsatellite markers are required for the whole genome survey in the fields of molecular ecology,quantitative genetics and genomics. Therefore,it is extremely necessary to select several versatile,low-cost,efficient and time-and labor-saving methods to develop a large panel of microsatellite markers. In this study,we used Zhikong scallop(Chlamys farreri) as the target species to compare the efficiency of the five methods derived from three strategies for microsatellite marker development. The results showed that the strategy of constructing small insert genomic DNA library resulted in poor efficiency,while the microsatellite-enriched strategy highly improved the isolation efficiency. Although the mining public database strategy is time-and cost-saving,it is difficult to obtain a large number of microsatellite markers,mainly due to the limited sequence data of non-model species deposited in public databases. Based on the results in this study,we recommend two methods,microsatellite-enriched library construction method and FIASCO-colony hybridization method,for large-scale microsatellite marker development. Both methods were derived from the microsatellite-enriched strategy. The experimental results obtained from Zhikong scallop also provide the reference for microsatellite marker development in other species with large genomes.
文摘The two-dimensional series patterns have been greatly applied to the changes of clothing details and edges, and the clothes with series edges is able to fully embody the clothing structure, highlight clothing modeling features, and modify the beauty of bodily form, so that clothes looks simple, decent, and cool, and its structure is clear to all; a form of rotation is formed on the human body, making the beauty-appreciation perspective of the viewers greatly changed. However, there are unending changes in the modern clothes, and therefore it is particularly important to explore a new design way of thinking in the field of design. The two-dimensional series patterns are reflected in the clothing details by relying on different composition forms, and can show the essence of traditional Chinese culture and promote design to win in details.
