To construct a DNA vaccine as a prophylactic model to prevent condyloma acuminatum and detect its immu-nogenicity in mice. Methods The major capsid protein (L1) gene of human papillomavirus (HPV) 6b was inserted into ...To construct a DNA vaccine as a prophylactic model to prevent condyloma acuminatum and detect its immu-nogenicity in mice. Methods The major capsid protein (L1) gene of human papillomavirus (HPV) 6b was inserted into an eukaryotic ex-pression plasmid (pcDNA3.1). The recombinant plasmid was transfected into COS-7 cells. Western blot were performed to detect whether L1 protein can be expressed in eukaryotic cells. Eighteen female BALB/c mice were tested for immunoge-nicity study. Results The recombinant plasmid (pcDNA3.1-HPV6bL1) was verified as HPV6b L1 gene by sequencing. Western blot showed specific strip. Anti-L1 protein antibodies could be detected in the mice’s sera inoculated with pcDNA3.1-HPV6bL1. Similarly, IL-4, IL-2, and IFN-γ were increased in the same mice. Conclusion HPV6b L1 recombinant plasmid was constructed successfully which had immunogenicity for BALB/c mice. It provided experimental evidence for the research of DNA vaccine of condyloma acuminata..-展开更多
N＋N nominal sentence is an important structure type of nominal sentences in Mandarin Chinese. Attributive-center, combination, apposition and subject-predicate are its main structure types. In main literary genres, ...N＋N nominal sentence is an important structure type of nominal sentences in Mandarin Chinese. Attributive-center, combination, apposition and subject-predicate are its main structure types. In main literary genres, the distribution of N＋N nominal sentence shows a certain trend of dominant hierarchy： poem﹥drama﹥novel﹥prose. No matter what kind of literary genres, attributive-center structure is the type with maximum quantity, while appositive structure is the type with minimum quantity. Statistical result indicates that most of N＋N nominal sentence is nominal and its use is limited by genres. Function of N＋N nominal sentence is textual. When it comes to discourse, it can be used as theme, rheme and dual identity of theme and rheme based on the theory of Theme-Rheme （T-R） structure pattern. It does not only construct the information structure to deliver textual information, but also its a vital means of discourse cohesion and coherence.展开更多
文摘To construct a DNA vaccine as a prophylactic model to prevent condyloma acuminatum and detect its immu-nogenicity in mice. Methods The major capsid protein (L1) gene of human papillomavirus (HPV) 6b was inserted into an eukaryotic ex-pression plasmid (pcDNA3.1). The recombinant plasmid was transfected into COS-7 cells. Western blot were performed to detect whether L1 protein can be expressed in eukaryotic cells. Eighteen female BALB/c mice were tested for immunoge-nicity study. Results The recombinant plasmid (pcDNA3.1-HPV6bL1) was verified as HPV6b L1 gene by sequencing. Western blot showed specific strip. Anti-L1 protein antibodies could be detected in the mice’s sera inoculated with pcDNA3.1-HPV6bL1. Similarly, IL-4, IL-2, and IFN-γ were increased in the same mice. Conclusion HPV6b L1 recombinant plasmid was constructed successfully which had immunogenicity for BALB/c mice. It provided experimental evidence for the research of DNA vaccine of condyloma acuminata..-
文摘N＋N nominal sentence is an important structure type of nominal sentences in Mandarin Chinese. Attributive-center, combination, apposition and subject-predicate are its main structure types. In main literary genres, the distribution of N＋N nominal sentence shows a certain trend of dominant hierarchy： poem﹥drama﹥novel﹥prose. No matter what kind of literary genres, attributive-center structure is the type with maximum quantity, while appositive structure is the type with minimum quantity. Statistical result indicates that most of N＋N nominal sentence is nominal and its use is limited by genres. Function of N＋N nominal sentence is textual. When it comes to discourse, it can be used as theme, rheme and dual identity of theme and rheme based on the theory of Theme-Rheme （T-R） structure pattern. It does not only construct the information structure to deliver textual information, but also its a vital means of discourse cohesion and coherence.
