对职高体育不同组织分组教学效果的研究

职高学生的生理和心理发展己达到相对稳定的水平,学生之间的个性差异也非常显著,而体育教学是多要素、多变的动态领域,在以教育目标引领教学内容为原则的前提下,对职高体育的组织方法和教学内容的选择要符合学生的个性化特征,是很值得探讨的问题。

初中化学分组实验的组织管理和评价

从分组实验课堂的组织管理，以及开发利用评价的激励、监管、约束功能等方面来阐述在分组实验教学实践中的思考和做法，以达到有效管理分组实验课堂，提高课堂效益的目的。

分组教学组织的类型

分组教学组织大致可分为2种类型：一种是外部分组型，一种是内部分组型。

AODV的本地修复算法

提出了一种新的路由本地修复算法,将修复尽量限制在断链的局部范围内,减少了修复时间和降低路由维护的开销,且不用考虑失效链路在整个路由上的相对位置,均可对其进行修复,提高了处理失效链路的能力,有利于提高网络的可扩展性。仿真表明,这种新的路由本地修复算法明显地提高了网络多方面的性能。

The Distribution of Repetitive DNAs Along Chromosomes in Plants Revealed by Self-genomic in situ Hybridization

The distribution of repetitive DNAs along chromosomes is one of the crucial elements for understanding the organization and the evolution of plant genomes. Using a modified genomic in situ hybridization （GISH） procedure, fluorescence in situ hybridization （FISH） with genomic DNA to their own chromosomes （called self-genomic in situ hybridization, self-GISH） was carried out in six selected plant species with different genome size and amount of repetitive DNA. Nonuniform distribution of the fluorescent labeled probe DNA was observed on the chromosomes of all the species that were tested. The signal patterns varied among species and were related to the genome size. The chromosomes of the small Arabidopsis genome were labeled almost only in the pericentromeric regions and the nucleolus organizer regions （NORs）. The signals in the relatively small genomes, rice, sorghum, and Brassica oleracea var. capitata L., were dispersed along the chromosome lengths, with a predominant distribution in the pericentromeric or proximal regions and some heterochromatic arms. All chromosomes of the large genomes, maize and barley, were densely labeled with strongly labeled regions and weakly labeled or unlabeled regions being arranged alternatively throughout the lengths. In addition, enhanced signal bands were shown in all pericentromeres and the NORs in B. oleracea var. capitata, and in all pericentromeric regions and certain intercalary sites in barley. The enhanced signal band pattern in barley was found consistent with the N-banding pattern of this species. The GISH with self-genomic DNA was compared with FISH with Cot-1 DNA in rice, and their signal patterns are found to be basically consistent. Our results showed that the self-GISH signals actually reflected the hybridization of genomic repetitive DNAs to the chromosomes, thus the self-GISH technique would be useful for revealing the distribution of the regions where repetitive DNAs concentrate along chromosomes and some chromatin differentiation associated with repetitive DNAs in plants.

Preliminary Study on Callus Differentiation of Hermaphrodite Papaya

With calluses of hermaphrodite papaya as the tested material and MS as the basic medium, the effects of different plant growth regulators and their combinations on adventitious bud induction of calluses and rooting induction of sterile buds were investigated. The results showed that 6-BA and TDZ all showed certain induction effect on callus differentiation of papaya; the induction effect of 6-BA was better than that of TDZ, and the optimum concentration of 6-BA was 0.05 mg/L. GA3 could promote the induction effect of 6-BA for on callus differentiation. The optimum medium combination for inducing the callus differentiation of papaya was MS ＋ 6- BA 0.5 mg/L ＋ GA3 1.0 mg/L. Compared to NAA, IBA was more suitable for inducing the rooting of adventitious buds. The optimum rooting-induction medium combination was MS ＋ IBA 0.3 mg/L.

DEC1 nuclear expression:A marker of differentiation grade in hepatocellular carcinoma

AIM: To investigate the expression patterns of human differentiated embryo chondrocyte 1 (DEC1) in hepatocellular carcinoma (HCC) and corresponding adjacent non-tumor and the normal liver tissues, the association between DEC1 expression and histopathological variables and the role of DEC1 in hepatocarcinogenesis. METHODS: The expression of DEC1 was detected immunohistochemically in 176 paraffin-embedded sections from 63 patients with HCC and 50 subjects with normal liver tissues. RESULTS: DEC1 protein was persistently expressed in the cytoplasm of hepatocytes in normal liver and HCC tissues. Compared with adjacent non-tumor liver tissues, HCC tissues showed high nuclear expression of DEC1 protein. However, high DEC1 nuclear expression was more frequently detected in well-differentiated (83.3%) than in moderately (27.3%) and poorly differentiated HCC (16.7%). Low DEC1 expression was associated with poor histological differentiation and malignancy progression. A correlation was found between the nuclear expression of DEC1 protein and histological differentiation (r = 0.376, P = 0.024). CONCLUSION: DEC1 is expressed in the cytoplasm of hepatocytes and because nuclear DEC1 expression is decreased with decreasing differentiation status of HCC, nuclear DEC1 might be a marker of HCC differentiation.

A Second Protein Marker of Caveolae:Caveolin-2

Caveolin-2,a protein about 20 kD,is a major component of the inner surface of caveolae,small invaginations of the plasma membrane.Similar with caveolin-1 and caveolin-3,it serves as a protein marker of caveolae.Caveolin-1 and-2 are located next to each other at 7q31.1 on human chromosome,the proteins encoded are co-localized and form a stable hetero-oligomeric complex,distributing similarly in tissue and cultured cells.Caveolin-3 is located on different chromosomes but confirmed to interact with caveolin-2.Caveolin-2 is similar to caveolin-1 in many respects but differs from the latter in functional domains,especially in G-protein binding domain and caveolin scaffolding domain.The mRNAs of both caveolin-1 and caveolin-2 are most abundantly expressed in white adipose tissue and are induced during differentiation of 3T3-L1 cells to adipocytes.Caveolin-2-deficinet mice demonstrate clear pulmonary defects,with little or no change in caveolin-1 expression and caveolae formation,suggesting that caveolin-2 plays a selective role in lung functions.Caveolin-2 is also involved in lipid metabolism and human cancers.

Spatiotemporal characteristics of the sea level anomaly in the Kuroshio Extension using a self-organizing map

Satellite altimeter SSH data in the Kuroshio Extension （KE） region gathered during the period January 1993 to December 2014 are analyzed using self-organizing map （SOM） analysis. Four spatial patterns （SOM1, SOM2, SOM3, and SOM4） are extracted, and the corresponding time series are used to characterize the variation of the sea level anomaly. Except in some individual months, SOM1 and SOM2 with single-branch jet structures appear alternately during the periods 1993-1998 and 2002-2011. However, during 1999-2001 and 2012-2014, SOM3 and SOM4 with double-branch jet structures are dominant.The sea level anomalies exhibit interannual variations, while the KE stream demonstrates decadal variation. For SOM1, the change in the KE path is less evident, although the KE jet is strong and narrow. For SOM2, the KE jet is weakened and widened and its jet axis moves towards the southwest. Compared with the SOM3, for SOM4 the trough and ridge in the upstream KE region are deeper in the northeast-southwest direction, and accompanied by a jet weakening and splitting.This study shows that SOM analysis is a useful approach for characterizing KE variability.

Survey of molecular profiling during human colon cancer development and progression by immunohistochemical staining on tissue microarray

AIM： To explore the molecular events taking place during human colon cancer development and progression through high-throughput tissue microarray analysis. METHODS： We constructed two separate tissue microarrays containing 1.0 mm or 1.5 mm cylindrical samples acquired from 112 formalin-fixed and paraffinembedded blocks, including carcinomas （n = 85）, adenomatous polyps （n = 18）, as well as normal paracancerous colon tissues （n = 9）. Immunohistochemical staining was applied to the analysis of the consecutive tissue microarray sections with antibodies for 11 different proteins, including p53, p21, bcl-2, bax, cyclin D1, PTEN, p-Aktl, β-catenin, c-myc, nm23-h1 and Cox-2. RESULTS： The protein expressions of p53, bcl-2, bax, cyclin D1, β-catenin, c-myc, Cox-2 and nm23-h1 varied significantly among tissues from cancer, adenomatous polyps and normal colon mucosa （P = 0.003, P = 0.001, P = 0.000, P = 0.000, P = 0.034, P = 0.003, P = 0.002, and P = 0.007, respectively）. Chi-square analysis showed that the statistically significant variables were p53, p21, bax, β-catenin, c-myc, PTEN, p-Aktl, Cox-2 and nm23-h1 for histological grade （P = 0.005, P = 0.013, P = 0.044, P = 0.000, P = 0.000, P = 0.029, P = 0.000, P = 0.008, and P = 0.000, respectively）, β-catenin, comyc and p-Akt1 for lymph node metastasis （P = 0.011, P =0.005, and P = 0.032, respectively）, β-catenin, c-myc, Cox-2 and nm23-h1 for distance metastasis （P = 0.020, P = 0.000, P = 0.026, and P = 0.008, respectively）, and cyclin D1, β-catenin, c-myc, Cox-2 and nm23h1 for clinical stages （P = 0.038, P = 0.008, P = 0.000, P = 0.016, and P = 0.014, respectively）. CONCLUSION： Tissue microarray immunohistochemical staining enables high-throughput analysis of genetic alterations contributing to human colon cancer development and progression. Our results implicate the potential roles of p53, cyclin D1, bcl-2, bax, Cox-2, β-catenin and c-myc in development of human colon cancer and that of bcl-2, nm23-h1, PTEN and p-Akt1 in progression of human colon cancer.

High level of ezrin expression in colorectal cancer tissues is closely related to tumor malignancy

AIM:To investigate the ezrin expression in normal colorectal mucosa and colorectal cancer tissues, and study the correlation between ezrin expression in colorectal cancer tissues and tumor invasion and metastasis.METHODS: Eighty paraffin-embedded cancer tissue samples were selected from primary colorectal adenocarcinoma. Twenty-eight patients had well-differentiated, 22 had moderately differentiated and 30 had poorly differentiated adenocarcinoma. Forty-five patients and 35 patients had lymph node metastasis. Forty-five patients were of Dukes A to B stage, and 35 were of C to D stage. Another 22 paraffi n-embedded tissue blocks of normal colorectal epithelium (>5 cm away from the edge of the tumor) were selected as the control group. All patients with colorectal cancer were treated surgically and diagnosed histologically, without preoperative chemotherapy or radiotherapy. The immunohistochemistry was used to detect the ezrin expression in paraffin-embedded normal colorectal mucosa tissues and colorectal cancer tissue samples.RESULTS: Ezrin expression in colorectal cancer was significantly higher than in normal colorectal mucosa (75.00% vs 9.09%, P<0.01), and there was a close relationship between ezrin expression and the degree of tumor differentiation, lymph node metastasis and Dukes stage (88.46% vs 50.00%, P<0.01; 94.28% vs 51.11%, P<0.01; 94.28% vs 51.11%, P<0.01).CONCLUSION: Ezrin expression is obviously higher in colorectal cancer tissues than in normal colorectal mucosa tissues, and the high level of ezrin expression is closely related to the colorectal cancer invasion and metastasis process.

Exuberant callus formation misdiagnosed as osteosarcoma:a case report

Reactive lesions of bone and soft tissue can appear alarming on histologic examination because they are often cellular and have atypical cytologic features,such as distinct nucleoli,mild hyperchromasia,and mitotic activity.Reactive lesions of bone and periosteum also produce bone and cartilage matrix,resulting in confusion with osteosarcoma or chondrosarcoma.Careful attention to key cytomorphological features such as the pattern of bone formation,uniform appearance of cells,and absence of atypical mitoses should help identify the reactive nature of a lesion.Correlation with clinical and radiological findings is also imperative to avoid misclassification of the tumor because reactive lesions often arise at sites where osteosarcoma and chondrosarcoma are rare(eg,the hand)and lack aggressive radiological features.Here we present a case of exuberant callus formation after avulsion fracture of tibia in a three year-old Iranian girl which misdiagnosed as osteosarcoma.

SPHERE： a scalable multicast framework in overlay networks

This paper presents Sphere, a scalable multicast framework in overlay network. Sphere is a highly efficient, serf-organizing and robust multicast protocol overlayed on the Internet. The main contrihutions of this paper are twofold. First, Sphere organizes the control topology of overlay network in two directions： horizontal and vertical. The horizontal meshes are used to locate and organize hosts in tracks, and the vertical meshes are. used to manage the data paths between tracks. Second, Sphere balances stress and stretch of the overlay network by assigning hosts into different tracks and clusters. This structure distributes stress on the muhicast trees uniformly, and meantime makes path stretch as small as possible. Simulations results show that Sphere can support multicast with large group size and has good performance on organizing meshes and building data delivery trees.

Spatial Organization Mechanism of Aircraft Manufacturing

Unquestionably,aircraft industry plays an important part in both economy and security of a nation.However,few of articles have dealt with the spatial development of this industry sector.The purpose of this article is to analyze the dynamics of agglomeration and diffusion of aircraft industry.In a historical view,with some spatial analysis methods,this research discusses the characteristics and patterns of aircraft industry's spatial organization and evolution,globally,nationally and regionally respectively.We find out there is a ‘Matthew effect' in aircraft industry of the world,and the spatial evolution of the industry is consistent with the nation's industrialization process.Then,it concludes that the main agglomeration forces consist of capital,talents,technology and cluster's advantages,and the main diffusion forces include comparative advantages,cost and risk sharing,emerging markets,development policy for backward regions and military requirements.All the factors can be divided into market forces making the spatial development of aircraft industry normal and non-market forces making that irregular.In particular,lessons from the USA and France are expected to be conducive to the rise of China's aircraft industry in the future.

Microarray expression analysis of epithelial ovarian cancer with distinct differentiation

To identify gene expression profiling in epithelial ovarian cancer and to explore its correlation with histopathology characterization and prognosis.Gene expression profiles were generated from 10 hu- man ovarian frozen tissue specimens using Agilent Human 1A microarrays.Strikingly.clear differences of gene expression patterns were observed in ovarian cancer as compared to normal tissues.Unique gene pro files were observed in moderately and poorly differentiated epithelial ovarian cancer.It is concluded that different histopathology characterization likely exists extensive molecular heterogeneity.

Case Study of Service Oriented Enterprise Architecture Framework

Many organizations have now adopted Service Oriented Architecture （SOA） as an architectural style to help them with architecture, design and implementation of their core services and systems. Most of these organizations are challenged in integrating SOA style with their overall Enterprise Architecture work. This framework links an SOA style with the Enterprise Architecture （EA） methodologies to help organizations organize their SOA effort as a key part of their Enterprise Architecture. The case study demonstrates the implementation of architecture goal with organization vision in service oriented organizational structure using services that align Business with Technology. The framework is validated and has reserved the privileges of SOA and EA.

Self-organized Critical Model Based on Complex Brain Networks with Hierarchical Organization

The dynamical behavior in the cortical brain network of macaque is studied by modelling each cortical areawith a subnetwork of interacting excitable neurons.We find that the avalanche of our model on different levels exhibitspower-law.Furthermore the power-law exponent of the distribution and the average avalanche size are affected by thetopology of the network.

Comparative proteomic study of colorectal carcinoma with different clinical stages

Objective： Colorectal carcinoma clinical stage associated proteins would be found by comparing differential expressed proteins from colorectal carcinoma tissues with different clinical stages. Methods： Total protein from colorectal carcinoma tissues were extracted; differential proteome profiles were established and analyzed by means of immobilized pH gradient-based two-dimensional polyacrylamide gel electrophoresis （2-DE） and matrix-assisted laser desorption/ionization time of flight mass spectrometry （MALDI-TOF-MS）. Results： Well-resolved, reproducible 2-DE profiles of human colorectal carcinoma tissues were obtained. Average protein spots were 970 ± 41,980 ± 32, 1010 ± 43, 1240 ±34 in stage Ⅰ, stage Ⅱ, stage Ⅲ, stage Ⅳ respectively; Compared to stage Ⅰ, differential expressed protein spots was 52.00 ± 12 in stage Ⅱ, 42.00 ± 11 in stage Ⅲ, 72.00 ± 15 in stage Ⅳ; Part of differential expressing proteins were analyzed by mass spectrometry and bioinformation, 19 of them were well characterized. Three proteins were overexpressed in stage Ⅰ, stage Ⅲ, stage Ⅳ, and one protein were overexpressed in stage Ⅳ exclusively. Conclusion： Differential expressed proteins exist in clinical stage of colorectal carcinoma, which would be biomarkers for diagnosis and prediction of prognosis.

Sleeping of a Complex Brain Networks with Hierarchical Organization

The dynamical behavior in the cortical brain network of macaque is studied by modeling each cortical area with a subnetwork of interacting excitable neurons. We characterize the system by studying how to perform the transition, which is now topology-dependent, from the active state to that with no activity. This could be a naive model for the wakening and sleeping of a brain-like system, i.e., a multi-component system with two different dynamical behavior.

Primary study on anther culture of balsum pear for callus and organ formation

The balsum pear （Momordica charantia L. ） anthers in the monokaryotic stage of microspore development were cultured in this experiment. Different Plant growth regulators＇ combinations, base media and carbon sources were studied for callus formation and organ differentiation from balsum pear anthers. The result showed that the best media for callus inducement was： MS＋BA 0.5 mg/L＋NAA 0.2 mg/L＋ 2, 4-D 0.5 mg/L＋KT 2.0 mg/L, with 3% sugar and 0.8% agar. The best media to induce roots from balsum pear anther callus was： MS＋NAA 0.05 rag/L＋ KT 0.5 rag/L, with 3% sugar and 0.8% agar. Most of adventitious roots from callus were triploid（2N=3X=33）