A novel technique of three-dimensional reconstruction segmentation and analysis for sliced images of biological tissues

A novel technique of three-dimensional (3D) reconstruction, segmentation, display and analysis of series slices of images including microscopic wide field optical sectioning by deconvolution method, cryo-electron microscope slices by Fou-rier-Bessel synthesis and electron tomography (ET), and a series of computed tomography (CT) was developed to perform si-multaneous measurement on the structure and function of biomedical samples. The paper presents the 3D reconstruction seg-mentation display and analysis results of pollen spore, chaperonin, virus, head, cervical bone, tibia and carpus. At the same time, it also puts forward some potential applications of the new technique in the biomedical realm.

广防风基原与鉴别

目的:建立广防风形态学及理化鉴别方法,完善质量标准。方法:考证广防风文献,应用植物分类学、药材鉴定学及薄层色谱的方法对广防风生药学鉴别特征观察记述,对不同产地广防风根及地上部位化学特征比对研究。结果:广防风本草考证表明古今广防风为唇形科植物广防风Epimeredi indica(L.)Rothm.干燥全草。本文提供广防风植物形态,根和茎叶性状特征,提供其组织横切面及粉末的特征,同时提供广防风地上与地下部位成分定性分析结果。结论:研究结果可为广防风的鉴别、质量标准制订及合理开发利用提供依据。