浅析组织文化建设与变革中的高校学生干部组织力培养机制

近年来,“00后”大学生逐渐成为高等学校的主力军,新时代新形势下高校学生组织正在经历深化改革,这使得“00后”学生干部队伍建设出现了诸多区别于以往大学生骨干培养的问题。学生干部既在学生组织内接受培养、成长成才,又是高校学生组织文化建设与变革的依托力量。“立大德,明大志,成大才,担大任”,学生干部的组织力培养成效是高校学生组织建设的重要目标,也是成果呈现。文章通过分析新时代学生干部的性格特点、组织文化建设与变革中面临的挑战,阐述高校学生干部组织力培养的重要性与核心内容要素,提出可行的培养机制。

籼粳稻成熟胚愈伤组织培养力的比较

以中籼品种扬稻6号和粳稻9908为实验材料,研究不同品种成熟胚(种子)愈伤组织的培养力.结果表明:以M8为基本培养基,激素配比为1 mg/L 2,4-D、2 mg/L ABA、2 mg/L 6-BA的培养基可以使扬稻6号的成愈率达到90.5%;激素配比为1 mg/L 2,4-D的培养基可以使9908的成愈率达到88.2%;以M8为基本继代培养基,激素配比为2 mg/L 2,4-D、1 mg/L ABA的培养基可以使扬稻6号和9908的愈伤组织的继代率分别为71.6%和87.5%;以MS为基本培养基,激素配比为0.2 mg/L 2,4-D、2 mg/L 6-BA、2 mg/L KT的培养基使扬稻6号和粳稻9908愈伤组织的植株再生分别达到65.4%%和62.7%,建立了扬稻6号和9908稳定高效的组织培养再生体系.

小麦幼胚组织培养力的遗传研究

在整个小麦幼胚培养周期内,愈伤组织的诱导、生长、根芽原基的形成和幼苗再生等各个阶段都受遗传机制所控制。多数研究表明,小麦幼胚培养力是受少数主基因控制的“质量性状”,同时也存在一些修饰基因,并且许多控制幼胚培养力的基因位于染色体B上,而且第2、4、6部分同源染色体群对幼胚培养力的影响较大。小麦幼胚培养力也与细胞质因子有关,特定的核质组合与培养条件的互作对幼胚培养力有密切的关系。多数研究者注意到,小麦幼胚培养力可能与小麦Rht和D3等矮源基因(dwarfinggene)有关,并认为这类基因可能通过植物激素代谢及渗透势变化等调控小麦幼胚培养力。

籼稻扬稻6号和粳稻苏沪香粳花药培养力的比较

为建立适合江苏水稻主栽品种花药培养的技术体系,本研究以籼稻扬稻6号和粳稻苏沪香粳为研究材料,分别从取样温度,低温预处理天数以及培养基中的蔗糖、琼脂和激素等研究不同水稻亚种花培的植株再生技术体系.结果表明:在气温26℃～31℃之间,扬稻6号在4℃低温预处理9d,接种到以M8为基本培养基,附加2,4-D1mg/L,NAA3mg/L,KT1mg/L,脯氨酸100mg/L,水解酪蛋白300mg/L,60g/L蔗糖和琼脂8g/L的花药愈伤诱导培养基中,可以获得高达8.5%的出愈率.而苏沪香粳在气温27℃～30℃之间,4℃低温预处理12d,接种到以M8为基本培养基,附加2,4-D1mg/L,NAA3mg/L,KT1mg/L,脯氨酸100mg/L,水解酪蛋白300mg/L,60g/L蔗糖和琼脂6g/L的花药愈伤诱导培养基中,可以获得高达15.05%的出愈率.扬稻6号和苏沪香粳花培愈伤组织转接分化培养基分别获得2.7%和5.7%的绿苗率.

水稻HD9802S/Kasalath后代中高组织培养力家系的筛选

HD9802S/Kasalath的杂交后代经多代农艺性状选择和自交纯合,共获得30个家系(291个选系)。对亲本、家系中高组织培养力相关的QTL(q Sv1和q Sc1-1)进行了检测,并对亲本、家系的成熟胚组织培养力进行了比较,结果发现亲本和家系均含有这2个QTL;HD9802S的愈伤组织诱导率低于Kasalath,绿苗分化率高于Kasalath,组织培养力与Kasalath相当;选出了9K18和9K19 2个组织培养力高的家系,其组织培养力分别为51.07%和54.80%,显著高于亲本HD9802S(26.88%)和Kasalath(27.12%),且其农艺性状明显优于Kasalath。

The Study on University Sport Education Reform based on Modular Teaching

Modular teaching is the international labor organization of research and development in the teaching Learn to give priority to, to ability training as the core function of a teaching mode. In our country higher education implementation of quality education during the course of this teaching mode, with its flexibility, Pertinence, realism, the characteristics of the economy, more and more attention, and started to come in The course reform of universities into practice. University public physical education has the distinct Health skill learning characteristics, the possibility of using modular teaching model, the university public Sports course target, knowledge system, skills requirements and the creativity of the students and the organic combination of the fitness ability. Through the reasonable design, construct a new type of male the sports course of platform, is to be based on the students, deepen university public physical education the reform process is worth areas of research.

Osteogenic potential of rabbit marrow stromal stem cells cultured in vitro: a hi stochemical and scanning electron microscopic study

To further investigate the osteogen ic potential of rabbit marrow stromal stem cells cultured in vitro. Methods: Rabbit marrow stromal stem cells were isolated by dens ity gradient centrifugation method and amplified in the flasks, using the osteog enic inducing conditions (OGC) as the culture media. The osteogenic potential of marrow stromal stem cells were investigated by means of bone seeking fluoresce nce (tetracycline) labelling, Alizarin red S (ARS) staining, Alcian blue Sirius red (AS) staining, and scanning electron microscope. Results: After being passaged, the marrow stromal stem cells in creased in number, became confluent and formed multi layer structure. The strom al stem cells excreted innumerable tiny granules, heaping up on the cell body an d merging gradually into foggy substances. These foggy substances kept on enlarg ing and formed round, oval, or flake like nodules. These nodules revealed brigh t golden yellow fluorescence under fluorescence microscope when labelled with te tracycline. Histochemical study with specific new bone staining with ARS reveale d positive calcium reaction, both denoting that they were newly formed bone tiss ues. After they were stained with AS, collagen and acid mucopolysaccharide were shown. Under scanning electron microscope, three types of cells with different c onfigurations were found. They were globular cells, spindle shaped cells and po lygonal or polygonal cells. Granules were excreted from the cells and heaped up on the cell body. Needle shaped and irregularly rectangular crystals also appea red and agglomerated with the granules to form nodules and trabecula like or fl ake like structures.Conclusions: Sequence of events of bone formation by rabbit mar row stromal stem cells cultured in vitro is fully depicted and confirmed, which provides the foundation for further investigating the mechanisms of osteoblast d ifferentiation from marrow stromal stem cells and the possible application in or thopaedics.