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猪断奶后多系统衰竭综合征诊断方法及标准的建立 被引量:13
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作者 崔尚金 李曦 +2 位作者 符芳 曲连东 童光志 《中国兽医科技》 CSCD 北大核心 2003年第11期68-72,共5页
关键词 断奶多系统衰竭综合征 症状 病理变化 诊断 PCR 间接免疫荧光试验 间接酶联免疫吸附试验 免疫组织化学试验
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珍香胶囊治疗子宫内膜异位症作用机制的初步研究
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作者 罗丽莉 曾如辉 +1 位作者 隋旭霞 傅玉才 《中国现代医学杂志》 CAS CSCD 北大核心 2008年第14期1999-2002,2021,共5页
目的探索中药珍香胶囊治疗大鼠子宫内膜异位症的机制。方法采用自体子宫移植方法建立大鼠子宫内膜异位症模型。4周后,将大鼠随机分组进行药物治疗(珍香胶囊组、内美通组、空白对照组)。治疗4周后,免疫组化方法检测大鼠异位内膜组织的雌... 目的探索中药珍香胶囊治疗大鼠子宫内膜异位症的机制。方法采用自体子宫移植方法建立大鼠子宫内膜异位症模型。4周后,将大鼠随机分组进行药物治疗(珍香胶囊组、内美通组、空白对照组)。治疗4周后,免疫组化方法检测大鼠异位内膜组织的雌激素(ER)α和β受体、血管内皮生长因子(VEGF)、芳香化酶(Aromatase)。ELISA方法检测大鼠血清、腹腔液中白介素(IL)-6、VEGF、肿瘤坏死因子(TNF)α。结果疗效:珍香胶囊能够抑制、破坏异位内膜的增长,其作用与内美通相似。ER-α、ER-β、VEGF、IL-6、Aro-matase在对照组及各用药组大鼠的移植组织中均有表达,各组之间无明显差异(P>0.05);ELISA检测各组大鼠血清、腹腔液中VEGF、IL-6、TNF-α水平,均未见阳性表达。结论珍香胶囊治疗大鼠子宫内膜异位症可能并不影响异位病灶组织的雌激素受体、血管内皮生长因子、芳香化酶,不影响大鼠血液、腹腔液的各种因子,其确切治疗机制目前尚不清楚。 展开更多
关键词 子宫内膜异位症 大鼠 珍香胶囊 内美通 酶联免疫吸附试验 免疫组织化学试验
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大鼠脊髓后角浅层内六种肽类和5-羟色胺的来源及其功能探讨 被引量:1
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作者 杜彦军 李继硕(指导) 秦秉志(指导) 《神经解剖学杂志》 CAS 1987年第1期101-106,145,共7页
分别切断大鼠一侧坐骨神经和半横切颈髓上段,用免疫组织化学技术观察了L_(4-6)后角浅层内SP、CCK、SOM、VIP、L-ENK、NT、5-HT等的量的改变。并半横切L_(5)节段的吻、尾侧,观察了NT在同节段内的含量改变。1.切断坐骨神经后,术侧L_(4-6)... 分别切断大鼠一侧坐骨神经和半横切颈髓上段,用免疫组织化学技术观察了L_(4-6)后角浅层内SP、CCK、SOM、VIP、L-ENK、NT、5-HT等的量的改变。并半横切L_(5)节段的吻、尾侧,观察了NT在同节段内的含量改变。1.切断坐骨神经后,术侧L_(4-6)后角浅层的坐骨神经占位区内SP、CCK、SOM、VIP有不同程度的脱失,SP、CCK最明显。结合文献,作者认为后根节中SP、CCK、SOM、VIP阳性神经元外周突部分分布于外周躯体神经。2.半横切颈髓后,术侧L_(4-5)后角浅层的5-HT阳性反应物基本消失,而L_(6)后角浅层内有少量存留。CCK、L-ENK阳性反庆物在L_(4-6)节段背外侧索中明显减少,但后角浅层只有轻微减少。SOM在术侧后角浅层中也似有轻微减少。这些提示脑结构到脊髓后角浅层的下行纤维中除含有5-HT外还含有一些神经肽。3.L—ENK阳性反应物在切断坐骨神经后轻度增高;SOM无论在切断坐骨神经或半横切劲髓例均变比不大;NT在此两组实验中均无变化,且在L_(5)节段吻、尾侧半切腰髓后L_(5)后角浅层内仍无变化。以上提示分布于后角浅层的某些肽类有一部分直接来自脊髓内甚至同节段内的固有神经元。 展开更多
关键词 脊髓后角浅层 SP CCK SOM VIP L-ENK NT 5-HT 免疫组织化学切断试验 大鼠
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植物形态实验方法的几点改进
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作者 郝建华 《庆阳师专学报(自然科学版)》 1994年第1期70-71,共2页
关键词 植物形态学 实验 徒手切片法 输导组织 解剖 组织化学试验
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猪流感的诊断
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作者 张太翔 Bruce H.Janke 《国外畜牧学(猪与禽)》 2001年第5期42-44,共3页
近年来有必要开发新的试验来检测猪流感并区分不同的病毒亚型
关键词 猪流感病毒 亚型 猪流行性感冒病毒 荧光抗体 免疫组织化学试验 血清样品 眼观病变
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Inhibition of high-mobility group box 1 expression by siRNA in rat hepatic stellate cells 被引量:14
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作者 Wen-Song Ge Jian-Xin Wu +2 位作者 Jian-Gao Fan Ying-Wei Chen Yao-Jun Wang 《World Journal of Gastroenterology》 SCIE CAS CSCD 2011年第36期4090-4098,共9页
AIM:To explore the role of high-mobility group box 1 (HMGB1) protein during liver fibrogenesis and investigate the functional effects of HMGB1 gene silencing in hepatic stellate cells (HSCs) using siRNA.METHODS:Hepati... AIM:To explore the role of high-mobility group box 1 (HMGB1) protein during liver fibrogenesis and investigate the functional effects of HMGB1 gene silencing in hepatic stellate cells (HSCs) using siRNA.METHODS:Hepatic fibrosis in rats was induced through serial subcutaneous injections of dimethylnitrosamine,and expression of HMGB1 was detected by immunohistochemistry.HMGB1 siRNAs were developed and transiently transfected into HSC-T6 cells using Lipofectamine 2000.HMGB1 expression was evaluated by real-time polymerase chain reaction (PCR) and Western blotting analysis.Expression of α-smooth muscle actin (α-SMA) and collagen typesⅠand Ⅲ was evaluated by real-time PCR.Cell proliferation and the cell cycle were determined using the methyl thiazolyl tetrazolium method.Finally,collagen content in HSC supernatant was evaluated by an enzyme-linked immunosorbent assay.RESULTS:The results showed that HMGB1 was upregulated during liver fibrosis and that its expression was closely correlated with the deposition of collagen.siRNA molecules were successfully transfected into HSCs and induced inhibition of HMGB1 expression in a time-dependent manner.Moreover,HMGB1 siRNA treatment inhibited synthesis of α-SMA and collagen types Ⅰ and Ⅲ in transfected HSCs.CONCLUSION:This study suggests a significant functional role for HMGB1 in the development of liver fibrosis.It also demonstrates that downregulation of HMGB1 expression might be a potential strategy to treat liver fibrosis. 展开更多
关键词 Hepatic fibrosis High-mobility group box 1 Hepatic stellate cells RNA interference
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A de novo germline MLH1 mutation in a Lynch syndrome patient with discordant immunohistochemical and molecular biology test results
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作者 Fabrice Airaud Sébastien Küry +4 位作者 Isabelle Valo Ingrid Maury Dominique Bonneau Olivier Ingster Stéphane Bezieau 《World Journal of Gastroenterology》 SCIE CAS CSCD 2012年第39期5635-5639,共5页
We describe a patient with a Homo sapiens mutL homolog 1 (MLH1)-associated Lynch syndrome with previous diagnoses of two distinct primary cancers:a sigmoid colon cancer at the age of 39 years, and a right colon cancer... We describe a patient with a Homo sapiens mutL homolog 1 (MLH1)-associated Lynch syndrome with previous diagnoses of two distinct primary cancers:a sigmoid colon cancer at the age of 39 years, and a right colon cancer at the age of 50 years. The mutation identified in his blood and buccal cells, c.1771delG, p.Asp591Ilefs*25, appears to be a de novo event, as it was not transmitted by either of his parents. This type of de novo event is rare in MLH1 as only three cases have been reported in the literature so far. Further-more, the discordant results observed between repli-cation error phenotyping and immunohistochemistry highlight the importance of the systematic use of both pre-screening tests in the molecular diagnosis of Lynch syndrome. 展开更多
关键词 Lynch syndrome Homo sapiens mutL ho-molog 1 De novo mutation Replication error pheno- type Immunohistochemistry
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Celiac disease markers in patients with liver diseases: A single center large scale screening study
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作者 Pavel Drastich Eva Honsová +7 位作者 Alena Lodererová Marcela Jaresová Aneta Pekáriková Iva Hoffmanová Ludmila Tuková Helena Tlaskalová-Hogenová Julius piák Daniel Sánchez 《World Journal of Gastroenterology》 SCIE CAS CSCD 2012年第43期6255-6262,共8页
AIM:To study the coincidence of celiac disease, we tested its serological markers in patients with various liver diseases.METHODS:Large-scale screening of serum antibodies against tissue transglutaminase (tTG), and de... AIM:To study the coincidence of celiac disease, we tested its serological markers in patients with various liver diseases.METHODS:Large-scale screening of serum antibodies against tissue transglutaminase (tTG), and deamidated gliadin using enzyme-linked immunosorbent assay and serum antibodies against endomysium using immunohistochemistry, in patients with various liver diseases (n = 962) and patients who underwent liver transplantation (OLTx, n = 523) was performed. The expression of tTG in liver tissue samples of patients simultaneously suffering from celiac disease and from various liver diseases using immunohistochemistry was carried out. The final diagnosis of celiac disease was confirmed by histological analysis of small-intestinal biopsy. RESULTS:We found that 29 of 962 patients (3%) with liver diseases and 5 of 523 patients (0.8%) who underwent OLTx were seropositive for IgA and IgG anti-tTG antibodies. However, celiac disease was biopsy-diagnosed in 16 patients:4 with autoimmune hepatitis type Ⅰ, 3 with Wilson's disease, 3 with celiac hepatitis, 2 with primary sclerosing cholangitis, 1 with primary biliary cirrhosis, 1 with Budd-Chiari syndrome, 1 with toxic hepatitis, and 1 with non-alcoholic steatohepatitis. Unexpectedly, the highest prevalence of celiac disease was found in patients with Wilson's disease (9.7%), with which it is only rarely associated. On the other hand, no OLTx patients were diagnosed with celiac disease in our study. A pilot study of the expression of tTG in liver tissue using immunohistochemistry documented the overexpression of this molecule in endothelial cells and periportal hepatocytes of patients simultaneously suffering from celiac disease and toxic hepatitis, primary sclerosing cholangitis or autoimmune hepatitis type Ⅰ. CONCLUSION:We suggest that screening for celiac disease may be beneficial not only in patients with associated liver diseases, but also in patients with Wilson's disease. 展开更多
关键词 Tissue transglutaminase Anti-tissue transglutaminase antibodies Autoimmune liver diseases Wilson's disease Celiac disease Liver transplantation
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用单因子血清识别急性致瘤性ALV诱发肿瘤组织中的fps肿瘤抗原
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作者 王一新 陈浩 +2 位作者 赵鹏 李建亮 崔治中 《微生物学报》 CAS CSCD 北大核心 2013年第3期299-305,共7页
【目的】制备鼠抗fps单因子血清,检测急性致瘤性ALV诱发肉瘤组织中的fps肿瘤抗原。【方法】以J亚群相关禽白血病/肉瘤病毒Fu-J株RNA为模板,分两段扩增v-fps肿瘤基因,利用大肠杆菌表达系统表达这两段蛋白,纯化后将两种蛋白同时免疫小鼠,... 【目的】制备鼠抗fps单因子血清,检测急性致瘤性ALV诱发肉瘤组织中的fps肿瘤抗原。【方法】以J亚群相关禽白血病/肉瘤病毒Fu-J株RNA为模板,分两段扩增v-fps肿瘤基因,利用大肠杆菌表达系统表达这两段蛋白,纯化后将两种蛋白同时免疫小鼠,获得抗血清,间接免疫荧光试验检测肉瘤组织滤过液感染的CEF和肿瘤细胞中的fps抗原,免疫组织化学方法检测肿瘤组织中的fps抗原。【结果】该单因子血清具有较好特异性,不与经典ALV-J发生交叉反应。被感染的CEF细胞、肿瘤细胞及纤维肉瘤组织切片均显示阳性。【结论】该实验制备了鼠抗fps单因子血清,建立了fps肿瘤抗原的检测方法,为进一步研究该病毒的生物学特性及其致瘤机制奠定了基础。 展开更多
关键词 J亚群相关禽白血病/肉瘤病毒 v-fps基因 单因子血清 间接免疫荧光试验 免疫组织化学试验
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猪断奶后多系统衰竭综合征诊断方法及标准的建立
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作者 崔尚金 李曦 +2 位作者 符芳 曲连东 童光志 《养猪大视野》 2005年第2期31-34,共4页
猪断奶后多系统衰竭综合征(PMWS)是一种由猪圆环病毒2型(Porcine circovirus 2,PCV2)引起的新病。PCV是1974年在猪肾传代细胞系PK-15中发现的一种污染病毒,该病毒不产生细胞病变效应(CPE),无致病性,命名为PCV1;而后在PMWS中分离的... 猪断奶后多系统衰竭综合征(PMWS)是一种由猪圆环病毒2型(Porcine circovirus 2,PCV2)引起的新病。PCV是1974年在猪肾传代细胞系PK-15中发现的一种污染病毒,该病毒不产生细胞病变效应(CPE),无致病性,命名为PCV1;而后在PMWS中分离的猪圆环病毒,有致病性,命名为PCV2。PMWS主要感染1~5月龄的猪,发病率为4%~30%,致死率为70%~80%。患病断奶猪和生长猪临床表现为进行性消瘦、呼吸困难、皮肤苍白、黄疸、腹泻和体表淋巴结肿大, 展开更多
关键词 猪断奶后多系统衰竭综合征 诊断方法 PMWS 临床症状 剖检变化 组织学变化 病毒 聚合酶链式反应 PCR 间接酶联免疫吸附试验 免疫组织化学试验
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