AIM: To investigate clinical and pathologic data of esophageal carcinoma (EC) and cardiac carcinoma (CC) among residents in Chaoshan region of China.METHODS: Clinical and pathologic data of 9 650 patients with EC and ...AIM: To investigate clinical and pathologic data of esophageal carcinoma (EC) and cardiac carcinoma (CC) among residents in Chaoshan region of China.METHODS: Clinical and pathologic data of 9 650 patients with EC and 4 173 patients with CC in the Chaoshan population were collected and analyzed. Moreover,Chaoshan esophageal carcinoma tissue arrays were made for high-throughput study.RESULTS: Male to female ratio was 3:1 in patients with EC and 4.75:1 in CC. The average age of the occurrenceof EC was 54.6 years, and of CC was 58.1 years. For both EC and CC, age at diagnosis was a little younger in Chaoshan region than in most other areas. The most commonly affected site of esophageal carcinoma was the middle third of esophagus (72.0%); the second was the lower third (15.3%). The main gross type of esophageal carcinoma was ulcerative type (41.50%); the medullary type was the second (39.6%). Squamous cell carcinoma accounted for the overwhelming majority of esophageal cancer (96.4%);adenocarcinoma accounted for the overwhelming majority of cardiac carcinoma (94.5%). Chaoshan esophageal carcinoma tissue arrays were easily for high-throughput study, and tissue cores with a diameter of 1.5 mm could better keep more structure for molecular expression study.CONCLUSION: Both EC and CC are common in males.The average occurrence age of EC and CC is younger in Chaoshan than in most other regions of China. The most commonly affected site of esophageal carcinoma was the middle third of esophagus (72.0%). Squamous cell carcinoma accounted for the overwhelming majority of esophageal cancer; adenocarcinoma accounted for the overwhelming majority of cardiac carcinoma. Tissue arrays technology is applicable for rapid molecular profiling of large numbers of cancers in a single experiment.展开更多
The ZM-1 tissue microarrayer designed by our groups is manufactured in stainless steel and brass and contains many features that make TMA (tissue microarray) paraffin blocks construction faster and more convenient. By...The ZM-1 tissue microarrayer designed by our groups is manufactured in stainless steel and brass and contains many features that make TMA (tissue microarray) paraffin blocks construction faster and more convenient. By means of ZM-1 tissue microarrayer, biopsy needles are used to punch the donor tissue specimens respectively. All the needles with the punched specimen cylinders are arrayed into the array-board, with an array of small holes dug to fit the needles. All the specimen cylinders arraying and the TMA paraffin block shaping are finished in only one step so that the specimen cylinders and the paraffin of the TMA block can very easily be incorporated and the recipient paraffin blocks need not be made in advance, and the paraffin used is the same as that for conventional pathology purpose. ZM-1 tissue microarrayer is easy to be manufactured, does not need any precision location system, and so is much cheaper than the currently used instrument. Our method’s relatively cheap and simple ZM-1 tissue microarrayer technique of constructing TMA paraffin block may facilitate popularization of the TMA technology.展开更多
The purpose of the present study was to analyse the changes of macromolecular organizations in nonjunctional sarcolemmas of different types of skeletal muscle fibres after cross-innervation. In normal rats the mean de...The purpose of the present study was to analyse the changes of macromolecular organizations in nonjunctional sarcolemmas of different types of skeletal muscle fibres after cross-innervation. In normal rats the mean density of square arrays (6 nm particles organized in orthogonal arrays) was 9.02/μm2 for the nonjunctional sarcolemmas of fast-twitch extensor digitorum longus (control EDL,CE) nuscle fibres and 0.34/μm2 for the nonjunctional sarcolemmas of slow-twitch soleus (control SOL, CS) muscle fibres. After cross-innervation between the fast-twitch EDL and slow-twitch SOL muscle fibres by slow and fast muscle merves respectively for three months, the mean density was 0.45/μm2 for the nonjunetional sarcolemmas of the operated-EDL (OE) and 8.3/μm2 for the nonjunctional sarcolemmas of the operated SOL (OS). This indicates that the cross-innervation causes a reciprocal transformation of the number and distribution of such macromolecular organizations in the electrically excitable nonjunctional sarcolemmas.展开更多
This paper presents a macroblock-level (MB-level) decoding and deblocking method for supporting the flexible macroblock ordering (FMO) and arbitrary slice ordering (ASO) bit streams in H.264 decoder and its SOC/ASIC i...This paper presents a macroblock-level (MB-level) decoding and deblocking method for supporting the flexible macroblock ordering (FMO) and arbitrary slice ordering (ASO) bit streams in H.264 decoder and its SOC/ASIC implementation. By searching the slice containing the current macroblock in the bit stream and switching slices correctly, MBs can be decoded in the raster scan order, while the decoding process can immediately begin as long as the slice containing the current MB is available. This architectural modification enables the MB-level decoding and deblocking 3-stage pipeline, and saves about 20% of SDRAM bandwidth. Implementation results showed that the design achieves real-time decoding of 1080HD (1920×1088@30 fps) at a system clock of 166 MHz.展开更多
基金Supported by the National Natural Science Foundation of China,No.30210103904 and Key Natural Science Foundation of Guangdong Province,No.A1080203 and Medical Research Foundation of Guangdong Province and Elitist Foundation of Guangdong,No.Q02109 and Deve
文摘AIM: To investigate clinical and pathologic data of esophageal carcinoma (EC) and cardiac carcinoma (CC) among residents in Chaoshan region of China.METHODS: Clinical and pathologic data of 9 650 patients with EC and 4 173 patients with CC in the Chaoshan population were collected and analyzed. Moreover,Chaoshan esophageal carcinoma tissue arrays were made for high-throughput study.RESULTS: Male to female ratio was 3:1 in patients with EC and 4.75:1 in CC. The average age of the occurrenceof EC was 54.6 years, and of CC was 58.1 years. For both EC and CC, age at diagnosis was a little younger in Chaoshan region than in most other areas. The most commonly affected site of esophageal carcinoma was the middle third of esophagus (72.0%); the second was the lower third (15.3%). The main gross type of esophageal carcinoma was ulcerative type (41.50%); the medullary type was the second (39.6%). Squamous cell carcinoma accounted for the overwhelming majority of esophageal cancer (96.4%);adenocarcinoma accounted for the overwhelming majority of cardiac carcinoma (94.5%). Chaoshan esophageal carcinoma tissue arrays were easily for high-throughput study, and tissue cores with a diameter of 1.5 mm could better keep more structure for molecular expression study.CONCLUSION: Both EC and CC are common in males.The average occurrence age of EC and CC is younger in Chaoshan than in most other regions of China. The most commonly affected site of esophageal carcinoma was the middle third of esophagus (72.0%). Squamous cell carcinoma accounted for the overwhelming majority of esophageal cancer; adenocarcinoma accounted for the overwhelming majority of cardiac carcinoma. Tissue arrays technology is applicable for rapid molecular profiling of large numbers of cancers in a single experiment.
基金Project (No. G1998051200) supported by the National Basic Re-search Program (973) of China
文摘The ZM-1 tissue microarrayer designed by our groups is manufactured in stainless steel and brass and contains many features that make TMA (tissue microarray) paraffin blocks construction faster and more convenient. By means of ZM-1 tissue microarrayer, biopsy needles are used to punch the donor tissue specimens respectively. All the needles with the punched specimen cylinders are arrayed into the array-board, with an array of small holes dug to fit the needles. All the specimen cylinders arraying and the TMA paraffin block shaping are finished in only one step so that the specimen cylinders and the paraffin of the TMA block can very easily be incorporated and the recipient paraffin blocks need not be made in advance, and the paraffin used is the same as that for conventional pathology purpose. ZM-1 tissue microarrayer is easy to be manufactured, does not need any precision location system, and so is much cheaper than the currently used instrument. Our method’s relatively cheap and simple ZM-1 tissue microarrayer technique of constructing TMA paraffin block may facilitate popularization of the TMA technology.
文摘The purpose of the present study was to analyse the changes of macromolecular organizations in nonjunctional sarcolemmas of different types of skeletal muscle fibres after cross-innervation. In normal rats the mean density of square arrays (6 nm particles organized in orthogonal arrays) was 9.02/μm2 for the nonjunctional sarcolemmas of fast-twitch extensor digitorum longus (control EDL,CE) nuscle fibres and 0.34/μm2 for the nonjunctional sarcolemmas of slow-twitch soleus (control SOL, CS) muscle fibres. After cross-innervation between the fast-twitch EDL and slow-twitch SOL muscle fibres by slow and fast muscle merves respectively for three months, the mean density was 0.45/μm2 for the nonjunetional sarcolemmas of the operated-EDL (OE) and 8.3/μm2 for the nonjunctional sarcolemmas of the operated SOL (OS). This indicates that the cross-innervation causes a reciprocal transformation of the number and distribution of such macromolecular organizations in the electrically excitable nonjunctional sarcolemmas.
基金Project (No. 2002AA1Z1190) supported by the National Hi-Tech Research and Development Program (863) of China
文摘This paper presents a macroblock-level (MB-level) decoding and deblocking method for supporting the flexible macroblock ordering (FMO) and arbitrary slice ordering (ASO) bit streams in H.264 decoder and its SOC/ASIC implementation. By searching the slice containing the current macroblock in the bit stream and switching slices correctly, MBs can be decoded in the raster scan order, while the decoding process can immediately begin as long as the slice containing the current MB is available. This architectural modification enables the MB-level decoding and deblocking 3-stage pipeline, and saves about 20% of SDRAM bandwidth. Implementation results showed that the design achieves real-time decoding of 1080HD (1920×1088@30 fps) at a system clock of 166 MHz.
