AIM To investigate the impact of IL28 B and OAS gene polymorphisms on interferon treatment responses in children with chronic hepatitis B.METHODS We enrolled 52 children(between the ages of 4 and 18) with hepatitis B ...AIM To investigate the impact of IL28 B and OAS gene polymorphisms on interferon treatment responses in children with chronic hepatitis B.METHODS We enrolled 52 children(between the ages of 4 and 18) with hepatitis B e antigen-negative chronic hepatitis B(CHB), who were treated with pegylated interferon alfa for 48 wk. Single nucleotide polymorphisms in the OAS1(rs1131476), OAS2(rs1293747),OAS3(rs2072136), OASL(rs10849829) and IL28B(rs12979860, rs12980275 and rs8099917) genes were studied to examine their associations with responses to IFN treatment in paediatric patients. We adopted two criteria for the therapeutic response, achieving an hepatitis B virus(HBV) DNA level < 2000 IU/m L and normalization of ALT activity(< 40 IU/L). To perform the analyses, we compared the patients in terms of achieving a partial response(PR) and a complete response(CR) upon measurement at the 24-wk posttreatment follow-up. RESULTS The PR and CR rates were 80.8% and 42.3%, respectively. Factors such as age, gender and liver histology had no impact on the type of response(partial or complete). A statistically significant relationship between higher baseline HBV DNA and ALT activity levels and lower rates of PR and CR was shown(P < 0.05). The allele association analysis revealed that only the IL-28 B rs12979860(C vs T) and IL28 B rs12980275(A vs G) markers significantly affected the achievement of PR(P = 0.021, OR = 3.3, 95%CI: 1.2-9.2 and P = 0.014, OR = 3.7, 95%CI: 1.3-10.1, respectively). However, in the genotype analysis, only IL-28 B rs12980275 was significantly associated with PR(AA vs AG-GG, P = 0.014, OR = 10.9, 95%CI: 1.3-93.9). The association analysis for CR showed that the TT genotype of IL28 B rs12979860 was present only in the no-CR group(P = 0.033) and the AA genotype of OASL rs10849829 was significantly more frequent in the noCR group(P = 0.044, OR = 0.26, 95%CI: 0.07-0.88). The haplotype analysis revealed significant associations between PR and CR and OAS haplotype(P = 0.0002 and P = 0.001, respectively), but no association with IL28 B haplotype was observed.CONCLUSION IL28 B and OAS polymorphisms are associated with different clinical outcomes in CHB children treated with interferon.展开更多
The accuracy of nucleation parameter is a critical factor in the simulation of microstructural evolution during dynamic recrystallization(DRX).Based on the flow stress curve under hot deformation conditions,a new appr...The accuracy of nucleation parameter is a critical factor in the simulation of microstructural evolution during dynamic recrystallization(DRX).Based on the flow stress curve under hot deformation conditions,a new approach is proposed to identify the nucleation parameter during DRX.In this approach,a cellular automaton(CA) model is applied to quantitatively simulate the microstructural evolution and flow stress during hot deformation;and adaptive response surface method(ARSM) is applied as optimization model to provide input parameters to CA model and evaluate the outputs of the latter.By taking an oxygen-free high-conductivity(OFHC) copper as an example,the good agreement between the simulation results and the experimental observations demonstrates the availability of the proposed method.展开更多
In the present study the thermal model of skin and subdermal tissues （SST） of human breast have been developed. The human breast is assumed to be spherical in shape with upper hemisphere projecting out from the trun...In the present study the thermal model of skin and subdermal tissues （SST） of human breast have been developed. The human breast is assumed to be spherical in shape with upper hemisphere projecting out from the trunk of the body and lower hemisphere is considered to be a part of the body core. The upper hemisphere represents the breast and its SST region is divided into three layers namely epidermis, dermis and subdermal tissues. The inner part of the breast represents the core/shell of the breast. The outer surface of the breast is assumed to be exposed to the environment from where the heat loss takes place by conduction, convection, radiation and evaporation. The heat transfer from core to the surface takes place by thermal conduction and blood perfusion. Also metabolic activity takes place at different rates in different SST layers of the breast. Boundary conditions have been framed on the basis of physical conditions. A finite element model has been developed for a two-dimensional steady state case.展开更多
文摘AIM To investigate the impact of IL28 B and OAS gene polymorphisms on interferon treatment responses in children with chronic hepatitis B.METHODS We enrolled 52 children(between the ages of 4 and 18) with hepatitis B e antigen-negative chronic hepatitis B(CHB), who were treated with pegylated interferon alfa for 48 wk. Single nucleotide polymorphisms in the OAS1(rs1131476), OAS2(rs1293747),OAS3(rs2072136), OASL(rs10849829) and IL28B(rs12979860, rs12980275 and rs8099917) genes were studied to examine their associations with responses to IFN treatment in paediatric patients. We adopted two criteria for the therapeutic response, achieving an hepatitis B virus(HBV) DNA level < 2000 IU/m L and normalization of ALT activity(< 40 IU/L). To perform the analyses, we compared the patients in terms of achieving a partial response(PR) and a complete response(CR) upon measurement at the 24-wk posttreatment follow-up. RESULTS The PR and CR rates were 80.8% and 42.3%, respectively. Factors such as age, gender and liver histology had no impact on the type of response(partial or complete). A statistically significant relationship between higher baseline HBV DNA and ALT activity levels and lower rates of PR and CR was shown(P < 0.05). The allele association analysis revealed that only the IL-28 B rs12979860(C vs T) and IL28 B rs12980275(A vs G) markers significantly affected the achievement of PR(P = 0.021, OR = 3.3, 95%CI: 1.2-9.2 and P = 0.014, OR = 3.7, 95%CI: 1.3-10.1, respectively). However, in the genotype analysis, only IL-28 B rs12980275 was significantly associated with PR(AA vs AG-GG, P = 0.014, OR = 10.9, 95%CI: 1.3-93.9). The association analysis for CR showed that the TT genotype of IL28 B rs12979860 was present only in the no-CR group(P = 0.033) and the AA genotype of OASL rs10849829 was significantly more frequent in the noCR group(P = 0.044, OR = 0.26, 95%CI: 0.07-0.88). The haplotype analysis revealed significant associations between PR and CR and OAS haplotype(P = 0.0002 and P = 0.001, respectively), but no association with IL28 B haplotype was observed.CONCLUSION IL28 B and OAS polymorphisms are associated with different clinical outcomes in CHB children treated with interferon.
基金Project(2006CB705401) supported by the National Basic Research Program of China
文摘The accuracy of nucleation parameter is a critical factor in the simulation of microstructural evolution during dynamic recrystallization(DRX).Based on the flow stress curve under hot deformation conditions,a new approach is proposed to identify the nucleation parameter during DRX.In this approach,a cellular automaton(CA) model is applied to quantitatively simulate the microstructural evolution and flow stress during hot deformation;and adaptive response surface method(ARSM) is applied as optimization model to provide input parameters to CA model and evaluate the outputs of the latter.By taking an oxygen-free high-conductivity(OFHC) copper as an example,the good agreement between the simulation results and the experimental observations demonstrates the availability of the proposed method.
文摘In the present study the thermal model of skin and subdermal tissues （SST） of human breast have been developed. The human breast is assumed to be spherical in shape with upper hemisphere projecting out from the trunk of the body and lower hemisphere is considered to be a part of the body core. The upper hemisphere represents the breast and its SST region is divided into three layers namely epidermis, dermis and subdermal tissues. The inner part of the breast represents the core/shell of the breast. The outer surface of the breast is assumed to be exposed to the environment from where the heat loss takes place by conduction, convection, radiation and evaporation. The heat transfer from core to the surface takes place by thermal conduction and blood perfusion. Also metabolic activity takes place at different rates in different SST layers of the breast. Boundary conditions have been framed on the basis of physical conditions. A finite element model has been developed for a two-dimensional steady state case.
