为研究猪精液热休克蛋白90(heat-shock protein 90,Hsp90)的定位以及表达特性,通过RT-PCR、蛋白质印迹、免疫组化、免疫荧光方法,检测和分析Hsp90在猪繁殖组织(睾丸和卵巢)、生殖细胞(精子细胞和卵母细胞)中的定位以及表达.RT-PCR结果显...为研究猪精液热休克蛋白90(heat-shock protein 90,Hsp90)的定位以及表达特性,通过RT-PCR、蛋白质印迹、免疫组化、免疫荧光方法,检测和分析Hsp90在猪繁殖组织(睾丸和卵巢)、生殖细胞(精子细胞和卵母细胞)中的定位以及表达.RT-PCR结果显示,Hsp90在睾丸和附睾尾表达,在卵巢中微量表达;蛋白质印迹结果显示,Hsp90蛋白在睾丸、卵巢、输卵管和尿道球腺中高表达;免疫组化结果显示,Hsp90蛋白在睾丸的间质细胞、次级精母细胞和精子细胞中表达,也在卵巢的卵母细胞、卵丘细胞和颗粒细胞中表达;免疫荧光结果显示,Hsp90蛋白定位在精子的颈部、尾中部、卵丘细胞的细胞质和卵母细胞中.综上所述,Hsp90在猪主要的繁殖组织和生殖细胞中高表达,其表达水平可能与精子质量和胚胎发育有关.展开更多
[Objective]The aim was to commercialize micropropagation of pistachio and provide good foundations for varieties improvement. [Method]The seeds and stem-segments were used as explants in the tissue culture of Pistacia...[Objective]The aim was to commercialize micropropagation of pistachio and provide good foundations for varieties improvement. [Method]The seeds and stem-segments were used as explants in the tissue culture of Pistacia vera ＇Kerman＇,tissue culture condition and medium and transplant matrix were researched for micropropagation of pistachio,[Result]Mediums of 1/2DKW and 1/2DKW＋3.00 mg/L of 6-BA ＋0.05 mg/L of NAA were suitable medium for seed germination and axillary bud induction of pistachio; Proliferation coefficient was 3.6 on the medium of 1/2DKW ＋4.00 mg/L of 6-BA ＋ 0.05 mg/L of IBA; Rooting rate was up to 75% on the medium of 1/2DKW ＋ 5.00 mg/L of IBA ＋ 1.00 mg/L of NAA; The suitable transplantation matrix was 3/4 sand ＋ 1/4 vermiculite,and survival rate of plant with root was above 79%. [Conclusion]The pistachio （Kerman） tissue culture technology system was established.展开更多
[Objective] The aim was to study the reproduction of the three-line genic male sterile （GMS） lineparent Mian7MB-1 （B. NapusL.） and the seed production of F1 through somatic tissue culture. [Methed] Through hybridi...[Objective] The aim was to study the reproduction of the three-line genic male sterile （GMS） lineparent Mian7MB-1 （B. NapusL.） and the seed production of F1 through somatic tissue culture. [Methed] Through hybridization, a new breeding material Mian 7MB-1 in three-line genic temporary maintainer line propagated by tissue culture was used to improve the sterile plant rate of rapeseed in dual-purpose recessive GMS line, such as Mian 7AB type, S45AB type, and etc. And then the variety comparative test was performed. [Result] In order to avoid some fertility restoration phenomena occurring during the process of self-reproduction, Mian 7AB was propagated in bulk with somatic tissue culture of temporary maintainer line plant stem. The propagated temporary maintainer line seedlings were applied to the breeding and seed production of net room male sterile line parent, promoting the sterile plant rate of the male sterile line parent to 91.7% -93.5%. The male sterile line parents per hectare were enough for the seed production of hybrid F1 in 7 500 -15 000 hm^2. [ Conclusion ] Compared with the original dual-purpose GMS line, the seed production ultilizing male sterile line with high sterile plant rate greatly reduced the labor, significantly improved the seed yield, ensuring the seed quality and forming a perfect breeding and seed production system.展开更多
[Objective] This study aimed to investigate rapid multiplication of Apocynum by tissue culture so as to provide plantlet sources for its industrialized cultivation. [Method] The asepsis seedlings were obtained by deal...[Objective] This study aimed to investigate rapid multiplication of Apocynum by tissue culture so as to provide plantlet sources for its industrialized cultivation. [Method] The asepsis seedlings were obtained by dealing with Apocynum seeds. Its cotyledons, hypocotyls and shoot tips were cultured on the media containing different concentrations of hormones. Finally, the influence of different hormone combinations on differentiation of cotyledons and hypocotyls, rapid multiplication of shoot tips, rapid multiplication of regenerated shoots, and rooting of test-tube plantlets was com- pared. [Result] MS＋2.0 mg/L BA＋0.03 mg/L NAA and MS＋0.07 mg/L NAA were the optimum medium for inducing regenerated buds from cotyledons and hypocotyls re- spectively; MS＋2.0 mg/L BA＋0.02 mg/L NAA was the best medium for rapid multi- plication of shoot tips; MS＋1.9 mg/L BA＋I.7 mg/L NAA was the best medium for rapid multiplication of regenerated buds： and 1/2MS＋0.6 mg/L NAA was the best medium for inducing roots. [Conclusion] The optimum hormone combination was de- termined for Apocynum rapid multiplication by tissue culture, which provides technical support on Apocynum industrialized cultivation.展开更多
Tissue culture has been widely used for mass propagation of Phalaenopsis. However, somaclonal variation occurred during micropropagation process posed a severe problem by affecting product quality. In this study, wild...Tissue culture has been widely used for mass propagation of Phalaenopsis. However, somaclonal variation occurred during micropropagation process posed a severe problem by affecting product quality. In this study, wild type and peloric flower buds of Phalaenopsis hybrids derived from flower stalk nodal culture were used for cDNA-RAPD and cDNA suppression subtractive hybridization analyses in order to study their genetic difference in terms of expressed sequence tags. A total of 209 ESTs from normal flower buds and 230 from mutants were sequenced. These ESTs sequences can be grouped into several functional categories involved in different cellular processes including metabolism, signal transduction, transcription, cell growth and division, protein synthesis, and protein localization, and into a subcat- egory of proteins with unknown function. Cymbidium mosaic virus transcript was surprisingly found expressed fre- quently in the peloric mutant of P. Little Mary. Real-time RT-PCR analysis on selected ESTs showed that in mutant flower buds, a bZIP transcription factor (TGA1a-like protein) was down-regulated, while up-regulated genes include auxin-regulated protein kinase, cyclophilin, and TCP-like genes. A retroelement clone was also preferentially expressed in the peloric mutant flowers. On the other hand, ESTs involved in DNA methylation, chromatin remodeling and post- transcriptional regulation, such as DNA methyltransferase, histone acetyltransferase, ERECTA, and DEAD/DEAH RNA helicase, were enriched in normal flower buds than the mutants. The enriched transcripts in the wild type indicate the down regulation of these transcripts in the mutants, and vice versa. The potential roles of the analyzed transcripts in the development of Phalaenopsis flowers are discussed.展开更多
文摘为研究猪精液热休克蛋白90(heat-shock protein 90,Hsp90)的定位以及表达特性,通过RT-PCR、蛋白质印迹、免疫组化、免疫荧光方法,检测和分析Hsp90在猪繁殖组织(睾丸和卵巢)、生殖细胞(精子细胞和卵母细胞)中的定位以及表达.RT-PCR结果显示,Hsp90在睾丸和附睾尾表达,在卵巢中微量表达;蛋白质印迹结果显示,Hsp90蛋白在睾丸、卵巢、输卵管和尿道球腺中高表达;免疫组化结果显示,Hsp90蛋白在睾丸的间质细胞、次级精母细胞和精子细胞中表达,也在卵巢的卵母细胞、卵丘细胞和颗粒细胞中表达;免疫荧光结果显示,Hsp90蛋白定位在精子的颈部、尾中部、卵丘细胞的细胞质和卵母细胞中.综上所述,Hsp90在猪主要的繁殖组织和生殖细胞中高表达,其表达水平可能与精子质量和胚胎发育有关.
基金Supported by Beijing Natural Science Foundation (6072005)Funding Project for Academic Human Resources Development in Institutions of Higher Learning Under the Jurisdiction of Beijing Municipality(PXM2009-014207-076874)~~
文摘[Objective]The aim was to commercialize micropropagation of pistachio and provide good foundations for varieties improvement. [Method]The seeds and stem-segments were used as explants in the tissue culture of Pistacia vera ＇Kerman＇,tissue culture condition and medium and transplant matrix were researched for micropropagation of pistachio,[Result]Mediums of 1/2DKW and 1/2DKW＋3.00 mg/L of 6-BA ＋0.05 mg/L of NAA were suitable medium for seed germination and axillary bud induction of pistachio; Proliferation coefficient was 3.6 on the medium of 1/2DKW ＋4.00 mg/L of 6-BA ＋ 0.05 mg/L of IBA; Rooting rate was up to 75% on the medium of 1/2DKW ＋ 5.00 mg/L of IBA ＋ 1.00 mg/L of NAA; The suitable transplantation matrix was 3/4 sand ＋ 1/4 vermiculite,and survival rate of plant with root was above 79%. [Conclusion]The pistachio （Kerman） tissue culture technology system was established.
基金Supported by "11thFive-Year" Crop Breeding Research of SichuanProvince "11thFive-Year" Joint Breeding Research Project Fun-ding of Sichuan Province.~~
文摘[Objective] The aim was to study the reproduction of the three-line genic male sterile （GMS） lineparent Mian7MB-1 （B. NapusL.） and the seed production of F1 through somatic tissue culture. [Methed] Through hybridization, a new breeding material Mian 7MB-1 in three-line genic temporary maintainer line propagated by tissue culture was used to improve the sterile plant rate of rapeseed in dual-purpose recessive GMS line, such as Mian 7AB type, S45AB type, and etc. And then the variety comparative test was performed. [Result] In order to avoid some fertility restoration phenomena occurring during the process of self-reproduction, Mian 7AB was propagated in bulk with somatic tissue culture of temporary maintainer line plant stem. The propagated temporary maintainer line seedlings were applied to the breeding and seed production of net room male sterile line parent, promoting the sterile plant rate of the male sterile line parent to 91.7% -93.5%. The male sterile line parents per hectare were enough for the seed production of hybrid F1 in 7 500 -15 000 hm^2. [ Conclusion ] Compared with the original dual-purpose GMS line, the seed production ultilizing male sterile line with high sterile plant rate greatly reduced the labor, significantly improved the seed yield, ensuring the seed quality and forming a perfect breeding and seed production system.
基金Supported by Science and Technology Development Program of Jilin Province(20110909)Youth Foud of Baicheng Normal University(200801)~~
文摘[Objective] This study aimed to investigate rapid multiplication of Apocynum by tissue culture so as to provide plantlet sources for its industrialized cultivation. [Method] The asepsis seedlings were obtained by dealing with Apocynum seeds. Its cotyledons, hypocotyls and shoot tips were cultured on the media containing different concentrations of hormones. Finally, the influence of different hormone combinations on differentiation of cotyledons and hypocotyls, rapid multiplication of shoot tips, rapid multiplication of regenerated shoots, and rooting of test-tube plantlets was com- pared. [Result] MS＋2.0 mg/L BA＋0.03 mg/L NAA and MS＋0.07 mg/L NAA were the optimum medium for inducing regenerated buds from cotyledons and hypocotyls re- spectively; MS＋2.0 mg/L BA＋0.02 mg/L NAA was the best medium for rapid multi- plication of shoot tips; MS＋1.9 mg/L BA＋I.7 mg/L NAA was the best medium for rapid multiplication of regenerated buds： and 1/2MS＋0.6 mg/L NAA was the best medium for inducing roots. [Conclusion] The optimum hormone combination was de- termined for Apocynum rapid multiplication by tissue culture, which provides technical support on Apocynum industrialized cultivation.
文摘Tissue culture has been widely used for mass propagation of Phalaenopsis. However, somaclonal variation occurred during micropropagation process posed a severe problem by affecting product quality. In this study, wild type and peloric flower buds of Phalaenopsis hybrids derived from flower stalk nodal culture were used for cDNA-RAPD and cDNA suppression subtractive hybridization analyses in order to study their genetic difference in terms of expressed sequence tags. A total of 209 ESTs from normal flower buds and 230 from mutants were sequenced. These ESTs sequences can be grouped into several functional categories involved in different cellular processes including metabolism, signal transduction, transcription, cell growth and division, protein synthesis, and protein localization, and into a subcat- egory of proteins with unknown function. Cymbidium mosaic virus transcript was surprisingly found expressed fre- quently in the peloric mutant of P. Little Mary. Real-time RT-PCR analysis on selected ESTs showed that in mutant flower buds, a bZIP transcription factor (TGA1a-like protein) was down-regulated, while up-regulated genes include auxin-regulated protein kinase, cyclophilin, and TCP-like genes. A retroelement clone was also preferentially expressed in the peloric mutant flowers. On the other hand, ESTs involved in DNA methylation, chromatin remodeling and post- transcriptional regulation, such as DNA methyltransferase, histone acetyltransferase, ERECTA, and DEAD/DEAH RNA helicase, were enriched in normal flower buds than the mutants. The enriched transcripts in the wild type indicate the down regulation of these transcripts in the mutants, and vice versa. The potential roles of the analyzed transcripts in the development of Phalaenopsis flowers are discussed.
