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紫外线对球结膜组织细胞因子分泌的影响
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作者 张俊华 金威尔 +1 位作者 张国安 林颖 《福建中医学院学报》 2002年第4期45-46,共2页
研究紫外线对球结膜组织细胞因子分泌的影响及清开灵的调控作用。利用近距离紫外线照射体外培养人球结膜组织 ,诱生细胞因子 ,并同时观察清开灵的调控作用。结果发现照射组的肿瘤坏死因子和血小板源生长因子水平明显升高 ;照射并加用清... 研究紫外线对球结膜组织细胞因子分泌的影响及清开灵的调控作用。利用近距离紫外线照射体外培养人球结膜组织 ,诱生细胞因子 ,并同时观察清开灵的调控作用。结果发现照射组的肿瘤坏死因子和血小板源生长因子水平明显升高 ;照射并加用清开灵组的细胞因子水平未见明显增高。结论 :近距离紫外线照射可诱导培养球结膜组织产生细胞因子 。 展开更多
关键词 紫外线 球结膜 组织细胞因子分泌 肿瘤坏死因子 血小板源生长因子 清开灵 翼状胬肉
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清开灵对翼状胬肉组织细胞因子分泌的影响
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作者 金威尔 张俊华 +1 位作者 林颖 张国安 《福建中医学院学报》 2001年第4期7-8,共2页
为研究清开灵对翼状胬肉组织细胞因子分泌的影响 ,利用组织培养技术培养翼状胬肉组织 ,采用放射免疫检测技术和细胞生物活性法 ,测定不同浓度的清开灵对翼状胬肉组织肿瘤坏死因子 ( TNF-α)和血小板源生长因子 ( PDGF)分泌水平的影响 ,... 为研究清开灵对翼状胬肉组织细胞因子分泌的影响 ,利用组织培养技术培养翼状胬肉组织 ,采用放射免疫检测技术和细胞生物活性法 ,测定不同浓度的清开灵对翼状胬肉组织肿瘤坏死因子 ( TNF-α)和血小板源生长因子 ( PDGF)分泌水平的影响 ,结果表明翼状胬肉组织培养上清液中的 TNF-α和 PDGF含量明显高于正常球结膜组 ,加入清开灵后 ,翼状胬肉的细胞因子分泌水平明显降低 ,且呈剂量依赖效应。说明清开灵是翼状胬肉组织细胞因子异常分泌的负性调节因子 ,可在翼状胬肉的防治中发挥重要作用。 展开更多
关键词 翼状胬肉 组织细胞因子 清开灵 中医药疗法
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组织细胞因子在溃疡性结肠炎患者中的表达水平
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作者 刘晓蕾 卢晓军 +3 位作者 葛海英 丁继东 徐薇 姜树原 《当代医学》 2022年第25期96-99,共4页
目的探讨组织细胞因子在溃疡性结肠炎患者中的表达水平。方法选取2017年6月至2019年3月中国人民解放军第九六九医院收治的70例溃疡性结肠炎患者作为观察组,另选取同期体检的70名健康者作为对照组。比较观察组不同分期与对照组组织细胞因... 目的探讨组织细胞因子在溃疡性结肠炎患者中的表达水平。方法选取2017年6月至2019年3月中国人民解放军第九六九医院收治的70例溃疡性结肠炎患者作为观察组,另选取同期体检的70名健康者作为对照组。比较观察组不同分期与对照组组织细胞因子[白细胞介素-1β(IL-1β)、白细胞介素-8(IL-8)及白细胞介素-17(IL-17)]的表达情况,比较观察组不同严重程度患者IL-1β、IL-8及IL-17表达情况。结果观察组活动期及缓解期IL-1β、IL-8及IL-17水平均明显高于对照组,且活动期患者IL-1β、IL-8及IL-17水平均高于缓解期患者,差异有统计学意义(P<0.05)。观察组重度患者IL-1β、IL-8及IL-17水平高于轻度患者及中度患者,且中度患者高于轻度患者,差异有统计学意义(P<0.05)。结论组织细胞因子在溃疡性结肠炎患者中呈高表达状态,且活动期和重度患者表达水平更高,临床可通过检测组织细胞因子明确诊断溃疡性结肠炎的不同分期和严重程度,并制订针对性治疗措施,临床应用价值较高。 展开更多
关键词 组织细胞因子 溃疡性结肠炎 分期 严重程度
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芳香化湿法对湿热下注型混合痔术后患者痔组织细胞因子VEGF、CD68、MMP9及免疫球蛋白的影响 被引量:4
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作者 郭海燕 《社区医学杂志》 2017年第5期59-62,共4页
目的探讨芳香化湿法对湿热下注型混合痔术后患者痔组织细胞因子VEGF、CD68、MMP9及免疫球蛋白的影响。方法选取2015年1月—2016年1月本院收治的102例湿热下注型混合痔术后患者,根据随机数字表法将患者分为对照组和观察组各51例,对照组... 目的探讨芳香化湿法对湿热下注型混合痔术后患者痔组织细胞因子VEGF、CD68、MMP9及免疫球蛋白的影响。方法选取2015年1月—2016年1月本院收治的102例湿热下注型混合痔术后患者,根据随机数字表法将患者分为对照组和观察组各51例,对照组采用痔疾洗液湿热敷,观察组采取芳香化湿方湿热敷,比较2组患者VEGF、CD68、MMP9的表达情况,手术前1 d及术后第14天采集患者的空腹静脉血测定2组患者IgM、IgA、IgG水平,同时评价2组患者的临床疗效。计数资料比较χ~2检验,计量资料比较t检验,P<0.05为差异有统计学意义。结果观察组VEGF、CD68、MMP9阳性率评分[(1.63±0.32)、(1.56±0.26)、(1.54±0.25)分]均明显低于对照组[(2.85±0.46)、(2.52±0.44)、(2.48±0.40)分],差异均有统计学意义(均P<0.05)。与术前1 d比较,术后第14天两组患者的IgM、IgA、IgG水平均明显降低,且对照组下降得更明显,差异均有统计学意义(均P<0.05)。观察组的总有效率为98.04%(50/51),显著高于对照组的84.31%(43/51),差异有统计学意义(P<0.05)。结论在湿热下注型混合痔术后患者中采用芳香化湿方湿热敷治疗,临床疗效确切,能有效抑制痔组织细胞因子VEGF、CD68、MMP9的表达,并在一定程度上修复患者的体液免疫功能。 展开更多
关键词 免疫球蛋白 组织细胞因子 湿热下注型混合痔 芳香化湿法
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溃疡性结肠炎大鼠实验模型的建立与评价 被引量:22
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作者 范恒 邱明义 《中医药学刊》 CAS 2004年第5期865-866,905,共3页
通过对溃疡性结肠炎 (UC)大鼠动物模型的一般情况 (包括大鼠的饮食、饮水、体重、大小便、皮毛、活动情况、精神状态等 )、结肠黏膜组织细胞因子 (IL -8、IL -6)、肠黏膜损伤积分、病理结构等方面的变化 ,来评价用 2 ,4-二硝基氯苯免疫... 通过对溃疡性结肠炎 (UC)大鼠动物模型的一般情况 (包括大鼠的饮食、饮水、体重、大小便、皮毛、活动情况、精神状态等 )、结肠黏膜组织细胞因子 (IL -8、IL -6)、肠黏膜损伤积分、病理结构等方面的变化 ,来评价用 2 ,4-二硝基氯苯免疫加醋酸局部灌肠法建立的大鼠模型 ,说明实验模型为研究UC的理想模型。 展开更多
关键词 溃疡性结肠炎 实验研究 动物模型 结肠黏膜组织细胞因子 肠黏膜损伤 病理结构
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Role of Tissue Factor Pathway Inhibitor-2 in Ovarian Tumor Migration and Invasion 被引量:2
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作者 仲任 黄瑞滨 宋善俊 《The Chinese-German Journal of Clinical Oncology》 CAS 2005年第1期53-55,68-69,共5页
Objective: To elucidate the relation between human tissue factor pathwayinhibitor-2 (TFPI-2) expression and ovarian tumor migration and invasion. Methods: Human TFPI-2expression vector pBos-Cite-neo/TFPI-2 was transfe... Objective: To elucidate the relation between human tissue factor pathwayinhibitor-2 (TFPI-2) expression and ovarian tumor migration and invasion. Methods: Human TFPI-2expression vector pBos-Cite-neo/TFPI-2 was transfected into ovarian tumor cells line A2780- Afterthe transfected cells were selected by G418, transfected and nontransfected cells were screened forTFPI-2 mRNA and protein by reverse transcription-polymerase chain reaction and Western blotanalysis, respectively. The number of transfected or nontransfected cells passing through membraneof Boyden chamber was counted as the basis assessing tumor cells migratory and invasive behaviors.Results: Expression of mRNA and protein of TFPI-2 was detectable in transfected cells. In invasionassay, the number of TFPI-2-expressing cells to traverse a Matrigel-coated membrane was obviouslydecreased compared with that of nonexpressing cells (59.3±6.5 vs 109.7±5.5, P 【 0.01); While inmigration assay, no significant difference through a noncoated membrane was observed amongtransfected and nontransfected cells (114.7±8.6 vs 127.3±7.1, P 】 0.05). Conclusion: Expression ofTFPI-2 may strongly inhibit the invasive ability of ovarian tumor cells in vitro, but has no effecton the migratory ability which provides an experimental basis for genotherapy of human ovariantumor. 展开更多
关键词 tissue factor pathway inhibitor-2 ovarian tumor INVASION MIGRATION
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Mesenchymal stem cells: a new strategy for immunosuppression and tissue repair 被引量:75
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作者 Yufang Shi Gangzheng Hu +11 位作者 Juanjuan Su Wenzhao Li Qing Chen Peishun Shou Chunliang Xu Xiaodong Chen Yin Huang Zhexin Zhu Xin Huang Xiaoyan Han Ningxia Xie Guangwen Ren 《Cell Research》 SCIE CAS CSCD 2010年第5期510-518,共9页
Mesenchymal stem cells (MSCs) have great potential for treating various diseases, especially those related to tissue damage involving immune reactions. Various studies have demonstrated that MSCs are strongly immuno... Mesenchymal stem cells (MSCs) have great potential for treating various diseases, especially those related to tissue damage involving immune reactions. Various studies have demonstrated that MSCs are strongly immunosuppressive in vitro and in vivo. Our recent studies have shown that un-stimulated MSCs are indeed incapable of immunosuppression; they become potently immunosuppressive upon stimulation with the supernatant of activated lymphocytes, or with combinations of IFN-γ, with TNF-α, IL-1α or IL-1β. This observation revealed that under certain circumstances, inflammatory cytokines can actually become immunosuppressive. We showed that there is a species variation in the mechanisms of MSC-mediated immunosuppression: immunosuppression by cytokine-primed mouse MSCs is mediated by nitric oxide (NO), whereas immunosuppression by cytokine-primed human MSCs is executed through indoleamine 2, 3-dioxygenase (IDO). Additionally, upon stimulation with the inflammatory cytokines, both mouse and human MSCs secrete several leukocyte chemokines that apparently serve to attract immune cells into the proximity with MSCs, where NO or IDO is predicted to be most active. Therefore, immunosuppression by inflammatory cytokine-stimulated MSCs occurs via the concerted action of chemokines and immune-inhibitory NO or IDO produced by MSCs. Thus, our results provide novel information about the mechanisms of MSC-mediated immunosuppression and for better application of MSCs in treating tissue injuries induced by immune responses. 展开更多
关键词 MSCS IMMUNOSUPPRESSION tissue repair immune diseases
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Connective tissue growth factor is overexpressed in human hepatocellular carcinoma and promotes cell invasion and growth 被引量:7
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作者 Ming Xiu Ya-Hui Liu +3 位作者 David R Brigstock Fang-Hui He Rui-Juan Zhang Run-Ping Gao 《World Journal of Gastroenterology》 SCIE CAS CSCD 2012年第47期7070-7078,共9页
AIM:To determine the expression characteristics of connective tissue growth factor(CTGF/CCN2) in human hepatocellular carcinoma(HCC) in histology and to elucidate the roles of CCN2 on hepatoma cell cycle progression a... AIM:To determine the expression characteristics of connective tissue growth factor(CTGF/CCN2) in human hepatocellular carcinoma(HCC) in histology and to elucidate the roles of CCN2 on hepatoma cell cycle progression and metastasis in vitro.METHODS:Liver samples from 36 patients(who underwent hepatic resection for the first HCC between 2006 and 2011) and 6 normal individuals were examined for transforming growth factor β1(TGF-β1) or CCN2 mRNA by in situ hybridization.Computer image analysis was performed to measure integrated optimal density of CCN2 mRNA-positive cells in carcinoma foci and the surrounding stroma.Fibroblast-specific protein-1(FSP-1) and E-cadherin were examined to evaluate the process of epithelial to mesenchymal transition,α-smooth muscle actin and FSP-1 were detected to identify hepatic stellate cells,and CD34 was measured to evaluate the extent of vascularization in liver tissues by immunohistochemical staining.CCN2 was assessed for its stimulation of HepG2 cell migration and invasion using commercial kits while flow cytometry was used to determine CCN2 effects on HepG2 cell-cycle.RESULTS:In situ hybridization analysis showed that TGF-β1 mRNA was mainly detected in connective tissues and vasculature around carcinoma foci.In comparison to normal controls,CCN2 mRNA was enhanced 1.9-fold in carcinoma foci(12.36 ± 6.08 vs 6.42 ± 2.35) or 9.4-fold in the surrounding stroma(60.27 ± 28.71 vs 6.42 ± 2.35),with concomitant expression of CCN2 and TGF-β1 mRNA in those areas.Epithelial-mesenchymal transition phenotype related with CCN2 was detected in 12/36(33.3%) of HCC liver samples at the edges between carcinoma foci and vasculature.Incubation of HepG2 cells with CCN2(100 ng/mL) resulted in more of the cells transitioning into S phase(23.85 ± 2.35 vs 10.94 ± 0.23),and induced a significant migratory(4.0-fold) and invasive(5.7-fold) effect.TGF-β1-induced cell invasion was abrogated by a neutralizing CCN2 antibody showing that CCN2 is a downstream mediator of TGF-β1-induced hepatoma cell invasion.CONCLUSION:These data support a role for CCN2 in the growth and metastasis of HCC and highlight CCN2 as a potential novel therapeutic target. 展开更多
关键词 Connective tissue growth factor Hepatocellular carcinoma Hepatoma cell line MIGRATION INVASION
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Foregut duplication cysts:A report of two cases with emphasis on embryogenesis 被引量:5
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作者 Thaer Khoury Louis Rivera 《World Journal of Gastroenterology》 SCIE CAS CSCD 2011年第1期130-134,共5页
Duplication cyst of the stomach with a pseudostratifie columnar ciliated epithelium is extremely rare.We de scribe two cases of these cysts,with emphasis on the immunophenotype and embryogenesis.The first patien was a... Duplication cyst of the stomach with a pseudostratifie columnar ciliated epithelium is extremely rare.We de scribe two cases of these cysts,with emphasis on the immunophenotype and embryogenesis.The first patien was a 29-year-old man who presented with crampin abdominal pain in his left lower quadrant.The secon patient was a 26-year-old woman who had a history over several years,of chronic epigastric abdominal pai radiating to her back.Both lesions were surgically re moved.They showed the same histomorphology.Th cysts were lined by a pseudostratified respiratory ep thelium with ciliated cells.The first cyst was connecte to the stomach,while the second cyst was not connect ed.Both cysts expressed thyroid transcription factor(TTF-1) and surfactant.In this report,we explore th possible embryogenesis of these lesions in the light o TTF-1 and surfactant expression. 展开更多
关键词 Duplication cyst STOMACH Thyroid transcription factor-1 SURFACTANT EMBRYOGENESIS
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Expression of albumin, IGF-1, IGFBP-3 in tumor tissues and adjacent non-tumor tissues of hepatocellular carcinoma patients with cirrhosis 被引量:7
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作者 Shi-Min Luo Wei-Min Tan +2 位作者 Wei-Xiong Deng Si-Min Zhuang Jian-Wei Luo 《World Journal of Gastroenterology》 SCIE CAS CSCD 2005年第27期4272-4276,共5页
AIM: To explore the expression of albumin (ALB), insulinlike growth factor (IGF)-I, and insulin-like growth factor binding protein (IGFBP)-3 in tumor tissues and adjacent non-tumor tissues of hepatocellular car... AIM: To explore the expression of albumin (ALB), insulinlike growth factor (IGF)-I, and insulin-like growth factor binding protein (IGFBP)-3 in tumor tissues and adjacent non-tumor tissues of hepatocellular carcinoma (HCC) patients with cirrhosis.METHODS: Twenty-four HCC patients with cirrhosis who underwent hepatectomy were studied. ALB mRNA, IGF-1 mRNA, and IGFBP-3 mRNA in liver tissues (including tumor tissues and adjacent non-tumor tissues) were detected by reverse transcriptase-polymerase chain reaction(RT-PCR). Liver Ki67 immunohistochemistry staining was studied. At the same time, 12 patients with cholelithiasis or liver angioma who underwent operation were segregated as normal control.RESULTS: In HCC patients with cirrhosis, hepatic ALB mRNA, IGF-1 mRNA, and IGFBP-3 mRNA of tumor tissues or adjacent non-tumor tissues were lower than the normal liver tissues, while in tumor tissues, hepatic ALB mRNA and IGFBP-3 mRNA were lower, hepatic IGF-1 mRNA was higher than in adjacent non-tumor tissues. Liver Ki67 labeling index (Ki67 LI) in tumor tissues or adjacent nontumor tissues were higher than that in the normal liver tissues, while in tumor tissues it was higher than that in adjacent non-tumor tissues.CONCLUSION: Imbalance of IGF-1 and IGFBP-3 may play a role in hepatocarcinogenesis and tumor development of liver cirrhosis patients. 展开更多
关键词 Hepatocellular carcinoma Insulin growth factor-i Insulin-like growth factor binding protein-3
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Connective tissue growth factor hammerhead ribozyme attenuates human hepatic stellate cell function 被引量:9
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作者 Run-Ping Gao David R Brigstock 《World Journal of Gastroenterology》 SCIE CAS CSCD 2009年第30期3807-3813,共7页
AIM: To determine the effect of hammerhead ribozyme targeting connective tissue growth factor (CCN2) on human hepatic stellate cell (HSC) function.METHODS: CCN2 hammerhead ribozyme cDNA plus two self-cleaving se... AIM: To determine the effect of hammerhead ribozyme targeting connective tissue growth factor (CCN2) on human hepatic stellate cell (HSC) function.METHODS: CCN2 hammerhead ribozyme cDNA plus two self-cleaving sequences were inserted into pTriEx2 to produce pTriCCN2-Rz. Each vector was individually transfected into cultured LX-2 human HSCs, which were then stimulated by addition of transforming growth factor (TGF)-β1 to the culture medium. Semiquantitative RT-PCR was used to determine mRNA levels for CCN2 or collagen I, while protein levels of each molecule in cell /ysates and conditioned medium were measured by ELISA. Cell-cycle progression of the transfected cells was assessed by flow cytometry.RESULTS: In pTriEx2-transfected LX-2 cells, TGF-β1 treatment caused an increase in the mRNA level for CCN2 or collagen I, and an increase in produced and secreted CCN2 or extracellular collagen I protein levels, pTriCCN2-Rz-transfected LX-2 cells showed decreased basal CCN2 or collagen mRNA levels, as well as produced and secreted CCN2 or collagen I protein. Furthermore, the TGF-β1-induced increase in mRNA or protein for CCN2 or collagen I was inhibited partially in pTriCCN2-Rz-transfected LX-2 cells. Inhibition of CCN2 using hammerhead ribozyme cDNA resulted in fewer of the cells transitioning into S phase.CONCLUSION: Endogenous CCN2 is a mediator of basal or TGF-β1-induced collagen I production in human HSCs and regulates entry of the cells into S phase. 展开更多
关键词 Connective tissue growth factor FIBROSIS Hepatic stellate cell Transforming growth factor-β1
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Study on Antithrombotic and Antiplatelet Activities of Low Molecular Weight Fucoidan from Laminaria japonica 被引量:4
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作者 CHEN Anjin ZHANG Fang +1 位作者 SHI Jie ZHAO Xue 《Journal of Ocean University of China》 SCIE CAS 2012年第2期236-240,共5页
The antithrombotic and antiplatelet effects of two fucoidan fractions with low molecular weight and different sulfate content from Laminaria japonica were compared in order to examine the influence of chemical charact... The antithrombotic and antiplatelet effects of two fucoidan fractions with low molecular weight and different sulfate content from Laminaria japonica were compared in order to examine the influence of chemical character on their antithrombotic activity and the possible mechanism. Both LMW fucoidan fractions exhibited favorable antithrombotic activity in an Fecl3-induced arterial thrombosis. The antithrombotic activity of LMW fucoidan was related with decrease of TXB2 and whole blood viscosity and hematocrit. LMW fucoidan showed a correlation between anticoagulant, antiaggregant and antithrombotic effects in vivo. For LMW fucoidan, antithrombotic activity required high dose of 5-10 nmol kg-1, concomitantly with increase in anticoagulant activity and inhibition of platelet aggregation. Administration of LMW fucoidan significantly promoted the 6-keto-PGF1α content and decreased the TXB2 content, indicating its inhibition of tissue factor pathway and regulation of metabolism of arachidonic acid. By comparison, highly sulfated fucoidan LF2 with Mw 3900 seemed to be a more suitable choice for antithrombotic drug for its antithrombotic activity accompanied with specific inhibitory activity on platelet aggregation, low anticoagulant activity and low hemorrhagic risk in vivo. 展开更多
关键词 FUCOIDAN ANTITHROMBOTIC COAGULATION ANTIPLATELET HEMORHEOLOGY
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PI3-K/PKB/NF-κB and p42/44 MAPK pathway mediates inhibition of lipoxin A_4 on CTGF-induced production of RANTES in mesangial cells 被引量:3
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作者 SHENG HUA WU CHAO LU LING DONG Guo PING ZHOU XIN You JIANG 《Journal of Microbiology and Immunology》 2005年第3期174-181,共8页
In order to investigate the regulatory role of connective tissue growth factor (CTGF) on production of RANTES (regulated on activation, normal T cell expressed and secreted) in rat glomerular mesangial cells, and ... In order to investigate the regulatory role of connective tissue growth factor (CTGF) on production of RANTES (regulated on activation, normal T cell expressed and secreted) in rat glomerular mesangial cells, and the modulatory effect of lipoxin A4 ( LXA4 ) on action of CTGF, and to explore the mechanisms of action of CTGF and LXA4, cultured rat mesangial cells were treated with CTGF, with or without preincubation with LXA4. Expression of mRNA was analyzed by RT-PCR. Protein of RANTES in the supematants was determined by ELISA. Monocyte transmigration was assessed by in vitro chemotaxis assay. Expression of p42/44 mitogen-activated protein kinase (MAPK), phosphoinositide 3-kinase ( PI3- K) and protein kinase B (PKB) was assessed by Western blotting. DNA-binding activity of nuclear factor-roB (NF-kB) was determined by electrophoretic mobility shift assay (EMSA). To observe whether transfection of LXA4 receptor homologue gene (LRHg) into mesangial cells intensified these modulatory effects of LXA4, mesangial cells were transfected with pcDNA3.1/LRHG vector. The results showed that CTGF enhanced the mRNA expression and protein release of RANTES, and the expression of phospho (P)-p42/44 MAPK, P-PI3-K, P-PKB and NF-kB. P-p42/44 MAPK blockade inhibited the CTgF-induced expression of P-p42/44 MAPK and partially decreased the level of RANTES in supematants. P- PI3-K blockade downregulated the CTGF-stimulated expression of P-PI3-K, P-PKB and NF-kB, and partially decreased the release of RANTES. NF-kB blockade abrogated the CTGF-activated NF-kB and partially decreased the secretion of RANTES. LXA4 dose-dependently inhibited the CTGF-stimulated above action. Transfection of LRHG into mesangial cells intensified these inhibitory effects of LXA4 on CTGFinduced release of RANTES and expression of the P-p42/44 MAPK. In conclusion, LXA4 inhibits CTGFinduced production of RANTES via PI3-K/PKB/NF-kB and p42/44 MAPK-dependent signal pathway, which is mediated by LRHG in rat mesangial cells. 展开更多
关键词 Lipoxin A4 Connective tissue growth factor RANTES Mesangial cells Nuclear factor-kB
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Prevention of Autoimmune Diabetes by Pentoxifylline is Associated with Target Tissue Modulation of Cytokines and the Expression of Fas-FasL
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作者 刘云峰 章毅 +2 位作者 任如枫 孙辽 张志利 《Journal of Microbiology and Immunology》 2004年第1期56-61,共6页
The effect of Pentoxifylline (PTX) on type 1 diabetes was investigated by means of the studies on the expressions of cytokine mRNA in pancreas and the Fas-FasL on islet cells of NOD mice. NOD mice were treated with PT... The effect of Pentoxifylline (PTX) on type 1 diabetes was investigated by means of the studies on the expressions of cytokine mRNA in pancreas and the Fas-FasL on islet cells of NOD mice. NOD mice were treated with PTX from 4-6?wk, and then from 8-12?wk. After treatment, it was found that the incidence of diabetes in NOD mice at ages of 30?wk was reduced to 25% in group of mice treated with PTX, in comparison to 73.3% in case of mice injected with PBS, and the degree of insulitis in the PTX treated mice was lower than that of the PBS injected mice. RT-PCR analysis revealed down-regulatory effect on the expressions of IFN-γ and TNF-α mRNA in PTX treated mice, but there was no any effect on the expression of IL-10. As to the expression of Fas, there was marked decrease in the mean cytoplasmic integral optical density (IOD) in PTX treated mice, but there was little difference between PTX and PBS groups in the expression of FasL. These results indicated that PTX could prevent the development of diabetes in NOD mice, which might be related to the regulation of Th1/Th2 imbalance and the decreased expression of Fas in islet cells. 展开更多
关键词 PENTOXIFYLLINE DIABETES CYTOKINE FAS-FASL
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大蒜新素治疗小鼠巨细胞病毒性心肌炎作用研究 被引量:8
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作者 徐翼 方峰 +4 位作者 董永绥 向稚丹 甄宏 易文龙 李革 《中华微生物学和免疫学杂志》 CAS CSCD 北大核心 2005年第6期476-480,共5页
目的探讨大蒜新素对小鼠巨细胞病毒(MCMV)性心肌炎治疗作用及其抗CMV机制。方法60只BALB/c小鼠随机分成大蒜新素治疗组(20只)、安慰剂组(20只)和正常对照组(20只)。大蒜新素治疗组接种MCMVK181后24h开始用大蒜新素一般剂量(25mg/kg)腹... 目的探讨大蒜新素对小鼠巨细胞病毒(MCMV)性心肌炎治疗作用及其抗CMV机制。方法60只BALB/c小鼠随机分成大蒜新素治疗组(20只)、安慰剂组(20只)和正常对照组(20只)。大蒜新素治疗组接种MCMVK181后24h开始用大蒜新素一般剂量(25mg/kg)腹腔注射,每天1次,共14d;安慰剂组和正常对照组仅用等量生理盐水。各组分别于治疗后第3、5、7、14天各处死5只小鼠,观察小鼠心肌组织病理损害;用双抗体夹心ELISA法检测小鼠心肌组织细胞因子IFNγ表达水平;用RTPCR方法检测小鼠脾核转录因子TbetmRNA表达强度。结果MCMV感染下调小鼠TbetmRNA和TH1类细胞因子IFNγ的表达(P<0.01);大蒜新素能诱导MCMV性心肌炎模型小鼠转录因子TbetmRNA和细胞因子IFNγ的表达显著增加(P<0.01),并显著改善MCMV感染小鼠心肌组织病理损害(P<0.05)。结论大蒜新素通过上调转录因子TbetmRNA表达进而促进TH1类细胞因子IFNγ分泌,诱导和促进TH1优势应答反应,增强机体特异性细胞免疫功能而发挥抗MCMV作用。 展开更多
关键词 病毒性心肌炎 作用研究 TH1类细胞因子 素治疗 IFN-γ T-bet BALB/c小鼠 ELISA法检测 RT-PCR方法 mRNA表达 小鼠巨细胞病毒 大蒜新素 CMV感染 组织细胞因子 细胞免疫功能 MCMV 病理损害 心肌组织 正常对照组 双抗体夹心
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Expression of VEGF-C and b-FGF in Lung Cancer and Its Relationship with Cancer Progress
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作者 Liu Yan Ma WenHao Ji Liang 《International English Education Research》 2014年第2期78-81,共4页
Objective: to observe expression of vascular endothelial growth factor-C (VEGF-C) and alkaline fibroblast growth factor (b-FGF) in tissues of lung cancer, and its relationship with cancer metastasis.Methods: to ... Objective: to observe expression of vascular endothelial growth factor-C (VEGF-C) and alkaline fibroblast growth factor (b-FGF) in tissues of lung cancer, and its relationship with cancer metastasis.Methods: to adopt immunohistochemical methods, analysis of 60 cases of lung tissue expression of VEGF-C and b-FGF in the situation.Result: positive rates of VEGF-C and b-FGF in lung cancer are respectively 56.67% and 63.33%; expression of VEGF-C and b-FGF in lung cancer is not related to pathological grades, pathologic stages or ages of patients (P 〉 0.05),but closely related to TNM stages and existence of lymph node metastasis (P 〈 0.01). IMVD in center of lung cancer tissues is obviously higher than surrounding area, with significant differences (P 〈 0.01). Conclusion: expression of VEGF-C and b-FGF is related to lung cancer progress. 展开更多
关键词 VEGF-C B-FGF lung cancer progress.
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Effects of valsartan on oxidative stress and the atherogenesis
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作者 陈钧 王琳 +3 位作者 陈欣 卜军 刘念 阮燕菲 《Journal of Medical Colleges of PLA(China)》 CAS 2003年第6期345-348,共4页
Objective: To investigate the therapy effect of valsartan on oxidative stress and the formation of atherosclerosis of rabbit. Methods: An atherosclerotic rabbit model was established by feeding high cholesterol diet s... Objective: To investigate the therapy effect of valsartan on oxidative stress and the formation of atherosclerosis of rabbit. Methods: An atherosclerotic rabbit model was established by feeding high cholesterol diet supplemented by bovine serum albumin injection bolus. The rabbits were randomly divided into the control, model, and valsartan treated group, six rabbits in each group. Blood samples were collected at the end of 8 weeks for examination of serum lipid levels and MDA levels; the aortas were harvested for histological morphometry analysis, vascular cell adhesion molecule-1 (VCAM-1) immunohistochemical analysis and in situ superoxide detection to reflect the activity of NAD(P)H oxidase. Results: Rabbits fed with high cholesterol diet showed higher serum lipids levels than those fed with normal diet(P<0.01). Treatment with valsartan (10 mg/kg per day) did not alter serum lipids levels. But the serum MDA level and ratio of lesion to intima area reduced significantly compared with model group(P<0.05). The expression of VCAM-1 decreased significantly in the valsartan treated group than in the model group (P<0.05).In addition, in situ superoxide detection also show the markedly reduction of superoxide as a result of valsartan treatment. Conclusion: These results indicate that the valsartan treatment can reduce the atherosclerotic progression, the mechanisms of which may include the inhibiting the NAD(P)H oxidase activity to produce superoxide and the downregulating the expression of redox sensitive genes in the downstream, such as VCAM-1. 展开更多
关键词 VALSARTAN ATHEROSCLEROSIS oxidative stress NAD(P)H oxidase
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TRANSFORMING GROWTH FACTOR-β AND FIBROBLAST GROWTH FACTOR INDUCE LENS EPITHELIAL EXPLANT METAPLASIA:IMPLICATIONS FOR THE FORMATION OF SUBCAPSULAR OPACIFICATION
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作者 刘颉 叶俊杰 《Chinese Medical Sciences Journal》 CAS CSCD 1998年第2期89-95,共7页
Objective. This study was to investigate the effects of transforming growth factor-β(TGFβ) and fi- broblast growth factor (FGF) in the subcapsular opacification formation of the lens. Methods. Lens epithelial explan... Objective. This study was to investigate the effects of transforming growth factor-β(TGFβ) and fi- broblast growth factor (FGF) in the subcapsular opacification formation of the lens. Methods. Lens epithelial explants from 10-day-old rats were cultured with TGFβ1 or TGFβ2 in the presence of FGF for 5 days, then were examined by light and electron microscopy, and by immunolocal- ization of smooth muscle(α-sm) actin and type I collagen. Results. In TGFβ/FGF-treated explants,extensive proliferation occured, with formation of spindle and star-shaped cells. These cells showed ultrastructure and biochemical features of fibroblast or myofibroblast. Prominent Golgi apparatus and rough endoplaic reticulum were observed in some cells. Intracellular micro- filaments with cytoplasmic dense babies and membrane associated dense bodies, features of smooth muscle cells, were also observed. Some cells showed reactivity to -sin actin antibody. TGFβ/FGF-treated ex- plants were strongly stained with type I collagen antibody. Condusion. In the presence of FGF, TGFβ1 and TGFβ2 induced lens epithelial cell (LEC ) proliferation and transformation into fibroblast or myofibroblast-like cells, with producing of abundant collagen matrix in the explants. The changes are similar to the metaplasia that occurrs in subcapsular opacification of the lens. The findings suggest that TGFβ and FGF plays a role in the pathogenesis of subcapsular opacification of the lens. 展开更多
关键词 transforming growth factor-β fibroblast growth factor LENS
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吉非替尼治疗老年晚期EGFR突变型非小细胞肺癌对MMP-9、TIMP-1及预后的影响
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作者 叶超 《中文科技期刊数据库(全文版)医药卫生》 2023年第9期77-80,共4页
探讨应用吉非替尼治疗老年晚期EGFR突变型非小细胞肺癌(NSCLC)对血液中的MMP-9和TIMP-1水平以及患者预后的影响。方法 纳入在2021年1月至2022年2月收治的50例老年晚期EGFR突变型NSCLC患者,应用随机数字表法的原则,分成观察组(n=25)、对... 探讨应用吉非替尼治疗老年晚期EGFR突变型非小细胞肺癌(NSCLC)对血液中的MMP-9和TIMP-1水平以及患者预后的影响。方法 纳入在2021年1月至2022年2月收治的50例老年晚期EGFR突变型NSCLC患者,应用随机数字表法的原则,分成观察组(n=25)、对照组(n=25)。对照组应用DP化疗(多西他赛+顺铂)方案,观察组在DP化疗基础上加用吉非替尼治疗方案,两组均治疗3个疗程。比较治疗前后血液中的肿瘤标志物MMP-9和TIMP-1的水平,并且比较治疗后1年的生存情况和复发转移率,评价治疗对患者的效果如何。结果 观察组经过治疗后,总有效率为88.00%,比对照组的56.00%要高(P<0.05)。经过3个疗程的治疗,观察组患者血液中的MMP-9和TIMP-1水平降低,且观察组的指标水平比对照组更低(P<0.05)。观察组的生存率为92.00%,高于对照组的56.00%,同时观察组的复发转移率为16.00%,低于对照组的48.00%,这些差异在统计学上具有显著意义(P<0.05)。结论 对于老年晚期肺癌患者中有EGFR基因突变的人来说,使用吉非替尼药物治疗效果很好,它能显著降低血液中MMP-9和TIMP-1的水平,提高患者的生存率,并降低肿瘤复发和转移的风险,这种治疗能够改善患者的预后情况。 展开更多
关键词 非小细胞肺癌 EGFR突变 老年 吉非替尼 基质金属蛋白酶9 组织细胞蛋白酶抑制因子1
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Monocyte-secreted Wnt5a interacts with FZD5 in microvascular endothelial cells and induces angiogenesis through tissue factor signaling 被引量:3
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作者 Gemma Arderiu Sonia Espinosa Esther Pena Rosa Aledo Lina Badimon 《Journal of Molecular Cell Biology》 SCIE CAS CSCD 2014年第5期380-393,共14页
Angiogenesis during reactive and pathologic processes is characteristically associated with inflammation. Inflammatory cells partici- pate in angiogenesis by secreting different molecules that affect endothelial celt ... Angiogenesis during reactive and pathologic processes is characteristically associated with inflammation. Inflammatory cells partici- pate in angiogenesis by secreting different molecules that affect endothelial celt functions. We had previously shown that induced tissue factor (TF) expression in activated rnicrovascular endothelial celts (rn EC) is able to induce angiogenesis via autocrine regulation. However, the signals that induce TF expression in mEC are not fully known. Here, we demonstrate that rnonocyte paracrine cross-talk with mECs triggers rnEC-TF expression. We have identified that rnonocyte-secreted Wnt5a induces TF expression in rnEC and function-ally induces celt rnonolayer repair and angiotube formation in vitro as well as rnicrovesset formation in vivo. Monocyte-secreted Wnt5a activates FZD5 in mECs, which signals to induce the release of intraceUular Ca2+ and increase NFKB transcription activity and TF gene expression. In sum, WntSa secreted by monocytes signals through the noncanonical Wnt-FZD5 pathway in mECs to induce TF expression that induces angiogenesis by autocrine regulation. 展开更多
关键词 WNT5A tissue factor microvascular endothelial cells MONOCYTES ANGIOGENESIS
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