期刊文献+
共找到3篇文章
< 1 >
每页显示 20 50 100
嗜水气单胞菌对鲫组织蛋白酶活性的影响
1
作者 朱越雄 曹广力 《内陆水产》 2002年第6期36-37,共2页
关键词 鲫鱼 嗜水气单胞菌 组织蛋白酶活性
下载PDF
家兔晶状体机械性损伤后虹膜和房水中组织金属蛋白酶抑制物活性的改变
2
作者 倪盈 姚克 +1 位作者 戴南平 吴仁毅 《中华眼科杂志》 CAS CSCD 北大核心 2004年第4期262-265,共4页
目的 探讨家兔晶状体机械性损伤后 ,基质金属蛋白酶抑制物 (TIMPs)在虹膜组织和房水中的动态变化及其意义。方法 建立单眼晶状体机械性损伤的家兔模型 ,应用反向酶谱法检测并定量分析损伤后 1、3、7和 15d眼内虹膜组织和房水中两种主... 目的 探讨家兔晶状体机械性损伤后 ,基质金属蛋白酶抑制物 (TIMPs)在虹膜组织和房水中的动态变化及其意义。方法 建立单眼晶状体机械性损伤的家兔模型 ,应用反向酶谱法检测并定量分析损伤后 1、3、7和 15d眼内虹膜组织和房水中两种主要的TIMPs (TIMP 1、2 )的活性 ,并应用蛋白酶谱法检测TIMPs的作用底物基质金属蛋白酶 (MMPs)的活性。结果 健康家兔眼和实验家兔未损伤眼的虹膜组织和房水中 ,均未检测到TIMP 1、2的相应蛋白活性条带。伤后 1d家兔损伤眼的虹膜组织中的TIMP 1、2活性显著增加 (P <0 0 5 ) ,而MMP 2的活性受抑制 ;伤后 1d损伤眼的房水中 ,TIMP 1、2的活性均显著增加 (P <0 0 5 ) ;此后逐渐减少 ,至伤后 7d与健康家兔比较无差异 (P值分别为 0 0 97和 0 777) ;酶谱分析结果显示MMP 2活性的改变与此对应 ,即在损伤后 1d受到抑制 ,此后逐渐恢复。结论 TIMPs参与家兔晶状体机械性损伤后的眼内急性反应 ,对抑制炎性反应、促进创伤修复可能具有重要意义。 展开更多
关键词 家兔 晶状体机械性损伤 虹膜 房水 组织金属蛋白酶抑制物活性 检测
原文传递
Effect of endothelin-1 receptor antagonists on histological and ultrastructural changes in the pancreas and trypsinogen activation in the early course of caerulein-induced acute pancreatitis in rats 被引量:3
3
作者 Anna Andrzejewska Jan W.Dlugosz Albert Augustynowicz 《World Journal of Gastroenterology》 SCIE CAS CSCD 2005年第8期1115-1121,共7页
AIM: To assess the effect of non-selective ETA/B (LU 302872)and selective ETA (LU 302146) antagonist on pancreatic histology and ultrastructure of acinar cells in connection with trypsinogen activation in early caerul... AIM: To assess the effect of non-selective ETA/B (LU 302872)and selective ETA (LU 302146) antagonist on pancreatic histology and ultrastructure of acinar cells in connection with trypsinogen activation in early caerulein-induced AP.METHODS: Male Wistar rats with caerulein-induced AP,lasting 4 h, were treated i.p. with 10 and 20 mg/kg b.w.of each antagonist. Edema, inflammatory infiltration,necrosis and vacuolization of acinar cells in the pancreas were scored at 0-3 scale. Free active trypsin (FAT), total potential trypsin (TPT) after activation with enterokinase,and index of trypsinogen activation (%FAT/TPT) were assayed in pancreatic homogenates.RESULTS: In untreated AP, the edema, inflammatory infiltration, necrosis and vacuolization increased as compared to control healthy rats (P<0.01). None of the treatment exerted any meaningful effect on the edema and inflammatory infiltration. The selective antagonist increased slightly the necrosis score to 0.82±0.06 at higher dose (P<0.05) vs 0.58±0.06 in untreated AP. The nonselective antagonist increased slightly the vacuolization score to 2.41±0.07 at higher dose (P<0.01) vs 1.88±0.08in untreated AP. The decrease in the number of zymogen granules, disorganization of endoplasmic reticulum,autophagosomes and cytoplasmic vacuoles were more prominent in treated AP than in untreated AP groups.%FAT/TPT in untreated AP increased about four times (18.4±3.8 vs4.8±1.3 in control group without AP, P<0.001).Treatment of AP with both antagonists did not affect significantly augmented trypsinogen activation.CONCLUSION: The treatment with endothelin-1 receptors (non-selective ETA/B and selective ETA) antagonists has essential effect neither on the edema and inflammatory infiltration nor on trypsinogen activation observed in the early course of caerulein-induced AP. Nevertheless a slight increase of the necrosis and vacuolization score and some of the ultrastructural data could suggest the possibility of their undesired effects in caerulein-induced AP at investigated doses. 展开更多
关键词 Acute pancreatitis CAERULEIN Endothelin-1 receptors antagonists Ultrastructure TRYPSIN
下载PDF
上一页 1 下一页 到第
使用帮助 返回顶部