The porcine major histocompatibility complex (MHC, also named swine leukocyte antigen, SLA) is associ- ated not only with immune responsibility and disease suscep- tibility, but also with some reproductive and product...The porcine major histocompatibility complex (MHC, also named swine leukocyte antigen, SLA) is associ- ated not only with immune responsibility and disease suscep- tibility, but also with some reproductive and productive traits such as growth rate and carcass composition. As yet system- atical research on SLA expression profile is not reported. In order to illustrate SLA expression comprehensively and deepen our understanding of its function, we outlined the expression profile of SLA in 51 tissues of Landrace by ana- lyzing a large amount of ESTs produced by “Sino-Danish Porcine Genome Project”. In addition, we also compared the expression profile of SLA in several tissues from different development stages and from another breed (Erhualian). The result shows: (i) classical SLA genes are highly expressed in immune tissues and middle part of intestine; (ii) although SLA-3 is an SLA Ia gene, its expression abundance and pat- tern are quite different from those of the other two SLA Ia genes. The same phenomenon is seen in HLA-C expression, suggesting that the two genes may function similarly and undergo convergent evolution; (iii) except in jejunum, the antigen presenting genes are more highly expressed in breed Erhualian than in Landrace. The difference might associate with the higher resistance to bad conditions (including pathogens) of Erhualian and higher growth rates of Land- race.展开更多
Objective:To study cellular compatibility of improved scaffold material with deproteinized heterogeneous bone and provide experimental basis on choosing the scaffold material in bone tissue engineering. Methods:Bone m...Objective:To study cellular compatibility of improved scaffold material with deproteinized heterogeneous bone and provide experimental basis on choosing the scaffold material in bone tissue engineering. Methods:Bone marrow stromal cells(BMSC)were co-cultured with heterogeneous deproteinized bone in vitro. The contrast phase microscope,scanning electron microscope,MTT assay,flow cytometry were performed and the BGP content and ALP activities were detected in order to observe the cell growth,adhesion in the material, cell cycle and cell viability. Results:The scaffold material of deproteinized heterogeneous bone had no inhibitory effect on cellular proliferation,differentiation and secretion function of BMSCs. Conclusions:The established heterogeneous deproteinized bone has good biocompatibility with BMSCs and is a potentially ideal scaffold material for bone tissue engineering.展开更多
文摘The porcine major histocompatibility complex (MHC, also named swine leukocyte antigen, SLA) is associ- ated not only with immune responsibility and disease suscep- tibility, but also with some reproductive and productive traits such as growth rate and carcass composition. As yet system- atical research on SLA expression profile is not reported. In order to illustrate SLA expression comprehensively and deepen our understanding of its function, we outlined the expression profile of SLA in 51 tissues of Landrace by ana- lyzing a large amount of ESTs produced by “Sino-Danish Porcine Genome Project”. In addition, we also compared the expression profile of SLA in several tissues from different development stages and from another breed (Erhualian). The result shows: (i) classical SLA genes are highly expressed in immune tissues and middle part of intestine; (ii) although SLA-3 is an SLA Ia gene, its expression abundance and pat- tern are quite different from those of the other two SLA Ia genes. The same phenomenon is seen in HLA-C expression, suggesting that the two genes may function similarly and undergo convergent evolution; (iii) except in jejunum, the antigen presenting genes are more highly expressed in breed Erhualian than in Landrace. The difference might associate with the higher resistance to bad conditions (including pathogens) of Erhualian and higher growth rates of Land- race.
文摘Objective:To study cellular compatibility of improved scaffold material with deproteinized heterogeneous bone and provide experimental basis on choosing the scaffold material in bone tissue engineering. Methods:Bone marrow stromal cells(BMSC)were co-cultured with heterogeneous deproteinized bone in vitro. The contrast phase microscope,scanning electron microscope,MTT assay,flow cytometry were performed and the BGP content and ALP activities were detected in order to observe the cell growth,adhesion in the material, cell cycle and cell viability. Results:The scaffold material of deproteinized heterogeneous bone had no inhibitory effect on cellular proliferation,differentiation and secretion function of BMSCs. Conclusions:The established heterogeneous deproteinized bone has good biocompatibility with BMSCs and is a potentially ideal scaffold material for bone tissue engineering.
