用地坑组芯法生产大型铸件的实践

介绍了采用地坑组芯造型方法生产大型非标铸钢件的工艺过程及生产工艺控制要点，并对地坑组芯造型与其他的造型工艺进行了比较，对生产效益进行了分析。

采用地坑组芯方法铸造大型薄壁件

根据铸件外形结构特征，从铸造工艺设计入手，结合车间生产条件，利用现有箱圈组成专用箱圈，采取地坑与组芯相结合的铸造方法，并制定出较为详细的造型与配箱方面的操作细则，使大型薄壁铸件一次浇注成功。

排钻底座铸件铸造工艺探析

介绍了排钻底座的结构特点及铸造工艺,重点论述了排钻底座成形中,对外观效果要求较高的“油槽沿部”的成形方式,选择合适的浇注系统,解决沿部出现的“冷隔”缺陷,以及密集的筋板成形中如何防止“掉砂”缺陷等,从对铸件质量的影响方面总结了各工艺要点的优点和不足。

High-performance single-chip exon capture allows accurate whole exome sequencing using the Illumina Genome Analyzer

Here we present an adaptation of NimbleGen 2.1M-probe array sequence capture for whole exome sequencing using the Illumina Genome Analyzer (GA) platform.The protocol involves two-stage library construction.The specificity of exome enrichment was approximately 80% with 95.6% even coverage of the 34 Mb target region at an average sequencing depth of 33-fold.Comparison of our results with whole genome shot-gun resequencing results showed that the exome SNP calls gave only 0.97% false positive and 6.27% false negative variants.Our protocol is also well suited for use with whole genome amplified DNA.The results presented here indicate that there is a promising future for large-scale population genomics and medical studies using a whole exome sequencing approach.