[ Objective] The study is to generate pharmaceutical protein via plant transgenic technique. [Methed] Using the cotyledons with petiole as transformation receptor, the fusion gene of rapeseed oil-body gene and bFGF wa...[ Objective] The study is to generate pharmaceutical protein via plant transgenic technique. [Methed] Using the cotyledons with petiole as transformation receptor, the fusion gene of rapeseed oil-body gene and bFGF was introduced into the rapeseed （ Brassica campestris L. ） by Agrobacterium tumefaciens-mediated transformation; meanwhile regeneration conditions of rapeseed were also optimized, and the regenerated resistant plantlets were detected by PCR and Southern blot. [ Result] This fusion gene had been integrated into rapeseed genome successfully, and the optimized conditions of transformation and regeneration were as follows： explants pre-culture for 2 d, co-culture for 3 d, bacteria solution OD600 for 0.3 and infection time for 5 min. [ Conclusion] The results laid a solid foundation for extraction, isolation and purification of protein in transgenic plant seeds.展开更多
Objective: We planned this study to investigate the relation between serum adiponectin level and hepatocellular carcinoma (HCC): risk, features and prognosis. Methods: The study included 100 patients with HCC and 40 h...Objective: We planned this study to investigate the relation between serum adiponectin level and hepatocellular carcinoma (HCC): risk, features and prognosis. Methods: The study included 100 patients with HCC and 40 healthy control subjects. Adiponectin levels were determined by an enzyme-linked immunosorbent assay kit. Results: In the subset of patients with compensated cirrhosis, the mean serum adiponectin level was significantly lower in HCC cases compared to healthy controls (88.6 versus 115 ng/mL; P = 0.012). In addition, serum adiponectin levels correlated negatively with tumor size (P = 0.004) and were significantly lower in patients with vascular invasion and distant metastases (P = 0.03 and P = 0.02 respectively). Furthermore, the median overall survival was significantly higher in the high adiponectin group than the low adiponectin group (median 12.5 versus 9.5 months; log rank = 4.6, P = 0.03). Conclusion: Decreased circulating adiponectin level may play a role in the development of HCC and is a potential poor prognostic marker. These data should be validated in further prospective studies. Also the mechanisms by which adiponectin affect the course of HCC need to be clarified.展开更多
A combined process of catalytic ozonation in the presence of a novel heterogeneous catalyst and biological activated carbon was investigated for the removal of priority control organic pollutants, the reduction of gen...A combined process of catalytic ozonation in the presence of a novel heterogeneous catalyst and biological activated carbon was investigated for the removal of priority control organic pollutants, the reduction of genotoxicity, and the improvement of biodegradable dissolved organic carbon (BDOC). Results confirm that the catalytic ozonation has higher effectiveness for the removal of refractory harmful organic pollutants, the reduction of genotoxicity and the increase of bio-degradability of organics than ozonation alone, which results in lower pollution load for subsequent biological activated carbon process, and then leads to less organic pollutants penetrating biological activated carbon. The novel catalytic ozonation with this combined process exhibits excellent performance to guarantee the safety of drinking water.展开更多
Highly efficient and rapid proteolytic digestion of proteins into peptides is a crucial step in shotgun-based proteome-analysis strategy. Tandem digestion by two or more proteases is demonstrated to be helpful for inc...Highly efficient and rapid proteolytic digestion of proteins into peptides is a crucial step in shotgun-based proteome-analysis strategy. Tandem digestion by two or more proteases is demonstrated to be helpful for increasing digestion efficiency and de- creasing missed cleavages, which results in more peptides that are compatible with mass-spectrometry analysis. Compared to conventional solution digestion, immobilized protease digestion has the obvious advantages of short digestion time, no self-proteolysis, and reusability. We proposed a multiple-immobilized proteases-digestion strategy that combines the ad- vantages of the two digestion strategies mentioned above. Graphene-oxide （GO）-based immobilized trypsin and endoprotein- ase Glu-C were prepared by covalently attaching them onto the GO surface. The prepared GO-trypsin and GO-Glu-C were successfully applied in standard protein digestion and multiple immobilized proteases digestion of total proteins of Thermoan- aerobacter tengcongensis. Compared to 12-hour solution digestion using trypsin or Glu-C, 14% and 7% improvement were obtained, respectively, in the sequence coverage of BSA by one-minute digestion using GO-trypsin and GO-GIu-C. Multiple immobilized-proteases digestion of the total proteins of Thermoanaerobacter tengcongensis showed 24.3% and 48.7% en- hancement in the numbers of identified proteins than was obtained using GO-trypsin or GO-Glu-C alone. The ultra-fast and highly efficient digestion can be contributed to the high loading capacity of protease on GO, which leads to fewer missed cleavages and more complete digestion. As a result, improved protein identification and sequence coverage can be expected.展开更多
The development of sensors for selective detection of cyanide ion(CN^-) is an important mission to accomplish because of the versatility and toxicity of CN^-. In the present work, an "ensemble"-based colorim...The development of sensors for selective detection of cyanide ion(CN^-) is an important mission to accomplish because of the versatility and toxicity of CN^-. In the present work, an "ensemble"-based colorimetric and fluorescent sensor(L2-Zn^(2+)) for CN^-ion has been developed. The addition of cyanide ions removed Zn^(2+) from the ensemble(L2-Zn^(2+)) in aqueous medium, resulting in a color change of the solution from red to buff and a "turn-on" fluorescent response. Also, the sensitivity of both the fluorescenceand colorimetric-based assay is below the maximum allowable level of cyanide ions in drinking water set by the World Health Organization. In addition, test strips, which served as convenient and efficient CN^- test kits, were fabricated based on the sensor.Notably, the selective detection of cyanide with L2-Zn^(2+) for practical application was also performed in sprouting potatoes.展开更多
Soil erosion, which includes soil detachment, transport, and deposition, is one of the important dynamic land surface processes. The magnetic tracer method is a useful method for studying soil erosion processes. In th...Soil erosion, which includes soil detachment, transport, and deposition, is one of the important dynamic land surface processes. The magnetic tracer method is a useful method for studying soil erosion processes. In this study, five types of magnetic tracers were made with fine soil, fly ash, cement, bentonite, and magnetic powder （reduced iron powder） using the method of disk granulation. The tracers were uniformly mixed with soil and tested in the laboratory using simulated rainfall and inflow experiments to simulate the interrill and rill components of soil erosion, in order to select one or more tracers which could be used to study detachment and deposition by the erosive forces of raindrops and surface flow of water on a slope. The results showed that the five types of magnetic tracers with high magnetic susceptibility and a wide range of sizes had a range of 0.99-1.29 gcm-3 in bulk density. In the interrill and rill experiments, the tracers FC1 and FC2 which consisted of fly ash and cement at ratios of 1：1 and 2：1, respectively, were transported in phase with soil particles since the magnetic susceptibility of sediment approximated that of the soil which was uneroded and the slopes of the regression equations between the detachment of sediment and magnetic tracers FC1 and FC2 were very close to the expected value of 20, which was the original soil/tracer ratio. The detachment and deposition on slopes could be accurately reflected by the magnetic susceptibility differences. The change in magnetic susceptibility depended on whether deposition or detachment occurred. However, the tracer FS which consisted of fine soil and the tracers FB1 and FB2 which consisted of fly ash and bentonite at ratios of 1：1 and 2：1, respectively, were all unsuitable for soil erosion study since there was no consistent relationship between sediment and tracer detachment for increasing amounts of runoff. Therefore, the tracers FC1 and FC2 could be used to study soil erosion by water.展开更多
基金Supported by Bioreactor Important Special Item of 863-Program inthe "Eleventh Five-Year" Plan (No. 2007AA100503)Science and Technology Development Key Plan of Jilin Province( No.20070922)+1 种基金Cultivation Fund of Scientific and Technical Innovation Project Major Program of Higher Education Institutions ( No.70S018)Science and Technology Plan of Changchun City (No.06GG150)~~
文摘[ Objective] The study is to generate pharmaceutical protein via plant transgenic technique. [Methed] Using the cotyledons with petiole as transformation receptor, the fusion gene of rapeseed oil-body gene and bFGF was introduced into the rapeseed （ Brassica campestris L. ） by Agrobacterium tumefaciens-mediated transformation; meanwhile regeneration conditions of rapeseed were also optimized, and the regenerated resistant plantlets were detected by PCR and Southern blot. [ Result] This fusion gene had been integrated into rapeseed genome successfully, and the optimized conditions of transformation and regeneration were as follows： explants pre-culture for 2 d, co-culture for 3 d, bacteria solution OD600 for 0.3 and infection time for 5 min. [ Conclusion] The results laid a solid foundation for extraction, isolation and purification of protein in transgenic plant seeds.
基金Supported by a grant from the Project Funding Unit, University of Mansoura, Egypt
文摘Objective: We planned this study to investigate the relation between serum adiponectin level and hepatocellular carcinoma (HCC): risk, features and prognosis. Methods: The study included 100 patients with HCC and 40 healthy control subjects. Adiponectin levels were determined by an enzyme-linked immunosorbent assay kit. Results: In the subset of patients with compensated cirrhosis, the mean serum adiponectin level was significantly lower in HCC cases compared to healthy controls (88.6 versus 115 ng/mL; P = 0.012). In addition, serum adiponectin levels correlated negatively with tumor size (P = 0.004) and were significantly lower in patients with vascular invasion and distant metastases (P = 0.03 and P = 0.02 respectively). Furthermore, the median overall survival was significantly higher in the high adiponectin group than the low adiponectin group (median 12.5 versus 9.5 months; log rank = 4.6, P = 0.03). Conclusion: Decreased circulating adiponectin level may play a role in the development of HCC and is a potential poor prognostic marker. These data should be validated in further prospective studies. Also the mechanisms by which adiponectin affect the course of HCC need to be clarified.
基金Sponsored by the National High Technology Research and Development Program (863) of China(Grant No. 2006AA06Z306)the National Natural Science Foundation of China(Grant No.50578051)
文摘A combined process of catalytic ozonation in the presence of a novel heterogeneous catalyst and biological activated carbon was investigated for the removal of priority control organic pollutants, the reduction of genotoxicity, and the improvement of biodegradable dissolved organic carbon (BDOC). Results confirm that the catalytic ozonation has higher effectiveness for the removal of refractory harmful organic pollutants, the reduction of genotoxicity and the increase of bio-degradability of organics than ozonation alone, which results in lower pollution load for subsequent biological activated carbon process, and then leads to less organic pollutants penetrating biological activated carbon. The novel catalytic ozonation with this combined process exhibits excellent performance to guarantee the safety of drinking water.
基金supported by the National Basic Research Program of China(2013CB911204)the National Key Scientific Instrument Development Program of China(2011YQ09000504)the National Natural Science Foundation of China(21275005,21235001)
文摘Highly efficient and rapid proteolytic digestion of proteins into peptides is a crucial step in shotgun-based proteome-analysis strategy. Tandem digestion by two or more proteases is demonstrated to be helpful for increasing digestion efficiency and de- creasing missed cleavages, which results in more peptides that are compatible with mass-spectrometry analysis. Compared to conventional solution digestion, immobilized protease digestion has the obvious advantages of short digestion time, no self-proteolysis, and reusability. We proposed a multiple-immobilized proteases-digestion strategy that combines the ad- vantages of the two digestion strategies mentioned above. Graphene-oxide （GO）-based immobilized trypsin and endoprotein- ase Glu-C were prepared by covalently attaching them onto the GO surface. The prepared GO-trypsin and GO-Glu-C were successfully applied in standard protein digestion and multiple immobilized proteases digestion of total proteins of Thermoan- aerobacter tengcongensis. Compared to 12-hour solution digestion using trypsin or Glu-C, 14% and 7% improvement were obtained, respectively, in the sequence coverage of BSA by one-minute digestion using GO-trypsin and GO-GIu-C. Multiple immobilized-proteases digestion of the total proteins of Thermoanaerobacter tengcongensis showed 24.3% and 48.7% en- hancement in the numbers of identified proteins than was obtained using GO-trypsin or GO-Glu-C alone. The ultra-fast and highly efficient digestion can be contributed to the high loading capacity of protease on GO, which leads to fewer missed cleavages and more complete digestion. As a result, improved protein identification and sequence coverage can be expected.
基金supported by the National Natural Science Foundation of China(21662031,21661028,21574104,21262032)the Program for Changjiang Scholars and Innovative Research Team in University of Ministry of Education of China(IRT 15R56)
文摘The development of sensors for selective detection of cyanide ion(CN^-) is an important mission to accomplish because of the versatility and toxicity of CN^-. In the present work, an "ensemble"-based colorimetric and fluorescent sensor(L2-Zn^(2+)) for CN^-ion has been developed. The addition of cyanide ions removed Zn^(2+) from the ensemble(L2-Zn^(2+)) in aqueous medium, resulting in a color change of the solution from red to buff and a "turn-on" fluorescent response. Also, the sensitivity of both the fluorescenceand colorimetric-based assay is below the maximum allowable level of cyanide ions in drinking water set by the World Health Organization. In addition, test strips, which served as convenient and efficient CN^- test kits, were fabricated based on the sensor.Notably, the selective detection of cyanide with L2-Zn^(2+) for practical application was also performed in sprouting potatoes.
基金Supported by the National Natural Science Foundation of China (No.40701094)the Award Foundation for Excellent Young Scientists of Shandong Province, China (No.BS2009NY025)
文摘Soil erosion, which includes soil detachment, transport, and deposition, is one of the important dynamic land surface processes. The magnetic tracer method is a useful method for studying soil erosion processes. In this study, five types of magnetic tracers were made with fine soil, fly ash, cement, bentonite, and magnetic powder （reduced iron powder） using the method of disk granulation. The tracers were uniformly mixed with soil and tested in the laboratory using simulated rainfall and inflow experiments to simulate the interrill and rill components of soil erosion, in order to select one or more tracers which could be used to study detachment and deposition by the erosive forces of raindrops and surface flow of water on a slope. The results showed that the five types of magnetic tracers with high magnetic susceptibility and a wide range of sizes had a range of 0.99-1.29 gcm-3 in bulk density. In the interrill and rill experiments, the tracers FC1 and FC2 which consisted of fly ash and cement at ratios of 1：1 and 2：1, respectively, were transported in phase with soil particles since the magnetic susceptibility of sediment approximated that of the soil which was uneroded and the slopes of the regression equations between the detachment of sediment and magnetic tracers FC1 and FC2 were very close to the expected value of 20, which was the original soil/tracer ratio. The detachment and deposition on slopes could be accurately reflected by the magnetic susceptibility differences. The change in magnetic susceptibility depended on whether deposition or detachment occurred. However, the tracer FS which consisted of fine soil and the tracers FB1 and FB2 which consisted of fly ash and bentonite at ratios of 1：1 and 2：1, respectively, were all unsuitable for soil erosion study since there was no consistent relationship between sediment and tracer detachment for increasing amounts of runoff. Therefore, the tracers FC1 and FC2 could be used to study soil erosion by water.
